Cytisine-type alkaloids and flavonoids from the rhizomes of <i>Sophora tonkinensis</i>

Pan, Qi-Ming; Zhang, Guijie; Huang, Ri-Zhen; Pan, Ying‐Ming; Wang, Hengshan; Liang, Dong

doi:10.1080/10286020.2015.1131680

Cited by 17 publications

(12 citation statements)

References 16 publications

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“…There have been few studies on the genetic diversity of Sophora tonkinensis with only one report of a phylogenetic analysis within the genus Euchresta that dealt with S. tonkinensis [6]. Many studies of S. tonkinensis were limited to its phytochemical constituents and pharmacological implications [7][8][9]. Data on the genetic diversity and structure of S. tonkinensis were the foundation for developing conservation strategies, which can provide insights for better utilization and conservation of genetic resources [10].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the genetic diversity and population structure of Sophora tonkinensis in South China by AFLP markers

Zhu

Xiao

Keovongkod

et al. 2020

Biotechnology & Biotechnological Equipment

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Sophora tonkinensis is an important source of herbal medicine for treatment of swelling and pain in the throat. Its chemical composition has been extensively studied; however, there are still gaps in the research on genetic diversity of S. tonkinensis which can provide insights for better utilization and conservation of genetic resources. In this study, 23 populations of diverse locations from southern China were evaluated using AFLP markers. The average number of alleles (Na and Ne) from all populations was 0.608 (Na) and 1.153 (Ne), average Shannon's information index was 0.137, which indicated that the genetic diversity of the whole populations was low. Unweighted pair-group method with arithmetic mean (UPGMA) clustering and principal coordinates analysis (PCoA) showed that the populations could be divided into three clades. The analysis of molecular variance (AMOVA) indicated that 20% of the molecular variance was due to differences among wild populations and 80% of the molecular variance was due to divergence within wild populations. There was a significant positive correlation between genetic distance and geographical distance, as well as ecological factors including altitude and temperature. It is necessary to closely monitor the status of the wild populations and consider expanding their genetic diversity. Establishment of the conservation basis in different areas is more conducive to the protection of S. tonkinensis.

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Section: Introductionmentioning

confidence: 99%

Assessment of the genetic diversity and population structure of Sophora tonkinensis in South China by AFLP markers

Zhu

Xiao

Keovongkod

et al. 2020

Biotechnology & Biotechnological Equipment

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“…In China, S. tonkinensis has been used as folk medicine to treat sore throat, acute pharyngolaryngeal infection, asthma, jaundice, and allergic dermatitis for hundreds of years [1,2]. Phytochemical investigations have shown that S. tonkinensis is rich in flavonoids [3,4,5,6,7,8], quinolizidine alkaloids [9,10,11], and arylbenzofurans [12,13]. Recent pharmacological studies have indicated that the flavonoids from S. tonkinensis showed pretty good anti-inflammatory [14,15,16], neuroprotective [17], hypoglycemic [18], anti-tumor [8,10], anti-allergic [12], and antioxidant [13] activities.…”

Section: Introductionmentioning

confidence: 99%

Preparative Purification of Total Flavonoids from Sophora tonkinensis Gagnep. by Macroporous Resin Column Chromatography and Comparative Analysis of Flavonoid Profiles by HPLC-PAD

Hou

Xiu

et al. 2019

Molecules

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For the full development and utilization of Sophora tonkinensis Gagnep., this study was primarily intended to established a simple and efficient approach for the preparative purification of total flavonoids from S. tonkinensis by macroporous resin column chromatography (MRCC). The adsorption and desorption characteristics of the total flavonoids on ten macroporous resins were first studied, and AB-8 resin was chosen as the most suitable, and the adsorption data were best fitted to the pseudo-second-order kinetics model and Langmuir isotherm model. Furthermore, the technological parameters for the purification of the total flavonoids were optimized using column chromatography. After a sample one-step purification procedure, the content of the total flavonoids increased by about 4.76-fold from 12.14% to 57.82%, with a recovery yield of 84.93%. In addition, the comparative analysis of the flavonoid extracts before and after purification was performed by high-performance liquid chromatography coupled with photodiode-array detection (HPLC-PAD). The results showed that the contents of six major flavonoids in the purified product were all higher than before the purification. Therefore, the AB-8 MRCC established in this work was a promising method for the industrial-scale purification of the total flavonoids from S. tonkinensis.

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“…N-formyl cytisine has also been studied for medicinal purposes [11]. At present, alkaloids in congeneric plants (Euchresta) were separated only by a single method such as silica gel column chromatography, thin-layer chromatography and sephadex LH-20 [12,13], which have obvious defects, and it is difficult to achieve the purpose of systematic separation and purification.…”

Section: Introductionmentioning

confidence: 99%

Systematic Separation and Purification of Alkaloids from Euchresta tubulosa Dunn. by Various Chromatographic Methods

2019

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High-speed countercurrent chromatography (HSCCC) and silica gel column chromatography were used to separate and purify alkaloids from Chinese herbal medicine Euchresta tubulosa Dunn. The purpose of this study is to provide a system mode for rapid separation of alkaloids from natural products. In the experiment, the eluent of silica gel column chromatography was screened by thin layer chromatography (TLC) to obtain four components with different polarity. Then, the two-phase solvent systems of different components were selected and purified by HSCCC. Four alkaloids with relatively high content were obtained by this mode successfully, including matrine (28 mg), oxymatrine (32 mg), N-formyl cytisine (24 mg), and cytisine (58 mg). The purity was higher than 91% by high performance liquid chromatography–ultraviolet (HPLC-UV) and their chemical structures were identified by nuclear magnetic resonance (NMR) and electron ionization mass spectrometry (EI-MS). The results showed that the combination of HSCCC and silica gel column chromatography could make alkaloids from natural products separate systematically.

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Cytisine-type alkaloids and flavonoids from the rhizomes of Sophora tonkinensis

Cited by 17 publications

References 16 publications

Assessment of the genetic diversity and population structure of Sophora tonkinensis in South China by AFLP markers

Assessment of the genetic diversity and population structure of Sophora tonkinensis in South China by AFLP markers

Preparative Purification of Total Flavonoids from Sophora tonkinensis Gagnep. by Macroporous Resin Column Chromatography and Comparative Analysis of Flavonoid Profiles by HPLC-PAD

Systematic Separation and Purification of Alkaloids from Euchresta tubulosa Dunn. by Various Chromatographic Methods

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