Cystic Fibrosis Transmembrane Regulator-independent Release of ATP

Watt, W. C.; Lazarowski, Eduardo R.; Boucher, Richard C.

doi:10.1074/jbc.273.22.14053

Cited by 154 publications

(46 citation statements)

References 36 publications

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“…This conclusion derives from the fact that P2Y 11 receptors are poorly stimulated by ADP (26), whereas our data show that ADP is at least as potent an agonist as ATP. In addition, ADP␤S also elicited a significant increase in [Ca 2ϩ ] i at a concentration of 10 M. In other airway epithelial cell models, the presence of P2Y 2 has already been demonstrated (16,58,59). Furthermore, in vivo studies demonstrate that aerosolized UTP has beneficial effects in treatment of CF lung disease, confirming the presence of P2Y 2 and/or P2Y 4 on the apical membrane of airway epithelium (23,48 volume regulation in IB3-1 cells.…”

Section: Discussionmentioning

confidence: 85%

Sustained Calcium Entry through P2X Nucleotide Receptor Channels in Human Airway Epithelial Cells

Zsembery

Boyce

Liang

et al. 2003

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 85%

Sustained Calcium Entry through P2X Nucleotide Receptor Channels in Human Airway Epithelial Cells

Zsembery

Boyce

Liang

et al. 2003

Journal of Biological Chemistry

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“…Direct measurement of ATP release by myosin functionalized tips of atomic force microscope reveals hot spots of ATP release on the surface of epithelial cells expressing CFTR (16). Other experimental reports (12,(17)(18)(19)(20) do not support this view, suggesting that the diameter of the pore of CFTR is too small to allow ATP to cross the plasma membrane. However, in this type of experiment, it is impossible to measure ATP release and ionic currents simultaneously in an individual cell, and so it was impossible to establish a direct relationship between the current activation and the release of ATP.…”

mentioning

confidence: 70%

ATP Crossing the Cell Plasma Membrane Generates an Ionic Current in Xenopus Oocytes

Bodas¹,

Aleu²,

Pujol

et al. 2000

Journal of Biological Chemistry

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The presence of ATP within cells is well established. However, ATP also operates as an intercellular signal via specific purinoceptors. Furthermore, nonsecretory cells can release ATP under certain experimental conditions. To measure ATP release and membrane currents from a single cell simultaneously, we used Xenopus oocytes. We simultaneously recorded membrane currents and luminescence. Here, we show that ATP release can be triggered in Xenopus oocytes by hyperpolarizing pulses. ATP release (3.2 ؎ 0.3 pmol/oocyte) generated a slow inward current (2.3 ؎ 0.1 A). During hyperpolarizing pulses, the permeability for ATP 4 -was more than 4000 times higher than that for Cl -. The sensitivity to GdCl 3 (0.2 mM) of hyperpolarization-induced ionic current, ATP release and E-ATPase activity suggests their dependence on stretch-activated ion channels. The pharmacological profile of the current inhibition coincides with the inhibition of ecto-ATPase activity. This enzyme is highly conserved among species, and in humans, it has been cloned and characterized as CD39. The translation, in Xenopus oocytes, of human CD39 mRNA encoding enhances the ATP-supported current, indicating that CD39 is directly or indirectly responsible for the electrodiffusion of ATP.

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“…ATP is abundant in the cell cytoplasm (3-5 mM) (1) and can be released extracellularly by several mechanisms including exocytosis of ATP-containing vesicles (1,(4)(5)(6)(7); transport via connexin hemichannels (8); or transport by nucleoside transporters, a process that may be regulated by the cystic fibrosis transmembrane conductance regulator (CFTR) (9)(10)(11)(12). Extracellular ATP binds to cell-surface purinergic receptors of the P2 class including the 8 transmembrane domain-containing P2Y receptors (P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 , P2Y 8 , P2Y 11 , P2Y 12 , and P2Y 13 isoforms) (13) and the ligand-gated ion-conducting P2X receptors, of which 7 receptor subunits have been described (P2X 1 -P2X 7 ) (14).…”

Section: Introductionmentioning

confidence: 99%

ATP and purinergic receptor–dependent membrane traffic in bladder umbrella cells

Wang¹,

Lee²,

Ruiz³

et al. 2005

J. Clin. Invest.

206

223

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The umbrella cells that line the bladder are mechanosensitive, and bladder filling increases the apical surface area of these cells; however, the upstream signals that regulate this process are unknown. Increased pressure stimulated ATP release from the isolated uroepithelium of rabbit bladders, which was blocked by inhibitors of vesicular transport, connexin hemichannels, ABC protein family members, and nucleoside transporters. Pressure-induced increases in membrane capacitance (a measure of apical plasma membrane surface area where 1 µF ≈ 1 cm 2 ) were inhibited by the serosal, but not mucosal, addition of apyrase or the purinergic receptor antagonist PPADS. Upon addition of purinergic receptor agonists, increased capacitance was observed even in the absence of pressure. Moreover, knockout mice lacking expression of P2X 2 and/or P2X 3 receptors failed to show increases in apical surface area when exposed to hydrostatic pressure. Treatments that prevented release of Ca 2+ from intracellular stores or activation of PKA blocked ATPγS-stimulated changes in capacitance. These results indicate that increased hydrostatic pressure stimulates release of ATP from the uroepithelium and that upon binding to P2X and possibly P2Y receptors on the umbrella cell, downstream Ca 2+ and PKA second messenger cascades may act to stimulate membrane insertion at the apical pole of these cells.

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Cystic Fibrosis Transmembrane Regulator-independent Release of ATP

Abstract: The cystic fibrosis (CF) transmembrane regulator (CFTR) is a cyclic AMP-dependent Cl

Cited by 154 publications

References 36 publications

Sustained Calcium Entry through P2X Nucleotide Receptor Channels in Human Airway Epithelial Cells

Sustained Calcium Entry through P2X Nucleotide Receptor Channels in Human Airway Epithelial Cells

ATP Crossing the Cell Plasma Membrane Generates an Ionic Current in Xenopus Oocytes

ATP and purinergic receptor–dependent membrane traffic in bladder umbrella cells

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