Cypermethrin-based formulation Barrage® induces histological changes in gills of the Pantanal endemic shrimp Macrobrachium pantanalense

Soares, Mayara Pereira; Oliveira, Natália Araújo de; Rebelo, D.; Marcondes, Sandriely F.; Fernandes, Carlos Eurico dos Santos; Domingues, Inês; Soares, Amadeu M.V.M.; Hayd, Liliam

doi:10.1016/j.etap.2019.01.014

Cited by 9 publications

(2 citation statements)

References 30 publications

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“…Cypermethrin is an insecticide that kills target organisms by blocking voltage‐gated sodium channels to affect the peripheral nervous system (Shafer et al, 2005). Derived from the insecticidal compounds found in chrysanthemum, cypermethrin is known to be more toxic to crustaceans than vertebrate fish species (Morolli et al, 2006; Sandodden & Johnsen, 2010; Soares et al, 2019), with mild toxicity present in amphibians and mammals (Edwards et al, 1986; Khan et al, 2009). Cypermethrin and related compounds have been shown to be effective at eradicating invasive crayfish populations at low doses (e.g., Sandodden & Johnsen, 2010), although it is also toxic to other aquatic organisms including benthic invertebrates (Zhang et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

Environmental DNA reveals invasive crayfish microbial associates and ecosystem‐wide biodiversity before and after eradication

et al. 2023

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Biodiversity monitoring in conservation projects is essential to understand environmental health, complexity, and recovery. However, traditional field surveys can be expensive, time‐consuming, biased toward visual detection, and/or only measure a limited set of taxa. Environmental DNA (eDNA) methods provide a new approach to biodiversity monitoring that has the potential to sample a taxonomically broader set of organisms with a similar effort, but many of these approaches are still in the early stages of development and testing. Here, we used multi‐locus eDNA metabarcoding to understand how the removal of invasive red swamp crayfish using cypermethrin pesticide impacts local biodiversity of a desert oasis ecosystem, as well as to detect crayfish both directly and indirectly. We tracked crayfish DNA signatures, microbial DNA associated with crayfish, and biodiversity of plant, fungal, animal, and bacterial communities through time. We were unsuccessful in detecting crayfish directly in either control tanks or oases using targeted metabarcoding primers for invertebrates and eukaryotes, similar to previous studies which have shown variable levels of success in detecting crayfish from environmental samples. However, we were successful in discerning a suite of 90 crayfish‐associated taxa to serve as candidate bioindicators of invasive presence using 16S and fungal ITS2 metabarcoding. Ranking these 90 taxa by their geographic distribution in eDNA surveys and by evidence of crayfish associations in the literature, we support nine taxa to be high ranking, and suggest they be prioritized in future biomonitoring. Biodiversity analyses from five metabarcode loci including plants, animals, and both prokaryotic and eukaryotic microbes showed that communities differed but that species richness remained relatively similar between oases through time. Our results reveal that, while there are limitations of eDNA approaches to detect crayfish and other invasive species, microbial bioindicators offer a largely untapped biomonitoring opportunity for invasive species management, adding a valuable resource to a conservation manager's toolkit.

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Section: Methodsmentioning

confidence: 99%

Environmental DNA reveals invasive crayfish microbial associates and ecosystem‐wide biodiversity before and after eradication

et al. 2023

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show abstract

“…Cypermethrin is an insecticide that kills target organisms by blocking voltage-gated sodium channels to affect the peripheral nervous system (Shafer et al, 2005). Derived from the insecticidal compounds found in chrysanthemum, cypermethrin is known be more toxic to crustaceans than vertebrate fish species (Morolli et al, 2006; Sandodden and Johnsen, 2010; Soares et al, 2019), with mild toxicity present in amphibians and mammals (Edwards et al, 1986; Khan et al, 2009). Cypermethrin and related compounds have been shown to be effective at eradicating invasive crayfish populations at low doses (e.g.…”

Section: Methodsmentioning

confidence: 99%

Environmental DNA reveals invasive crayfish microbial associates and ecosystem-wide biodiversity before and after eradication

Ballare

Worth

Ruiz‐Ramos

et al. 2022

Preprint

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Biodiversity monitoring in conservation projects is essential to understand environmental status and recovery. However, traditional field surveys can be biased towards visual detection and/or focused on measuring the biodiversity of a limited set of taxa. Environmental DNA (eDNA) methods provide a new approach to biodiversity monitoring that has the potential to sample a taxonomically broader set of organisms with similar effort, but these approaches are still in the early stages of development and testing. Here, we explore the utility of multilocus eDNA metabarcoding to explore the impact on local biodiversity of removal of the red swamp crayfish, a globally invasive species, from a desert oasis ecosystem. We tracked crayfish DNA signatures, microbial DNA associated with crayfish, and biodiversity changes of plant, fungal, animal, and bacterial communities through time. We were unsuccessful in detecting crayfish in control tanks or oases using targeted metabarcoding primers for invertebrates and eukaryotes. Metabarcoding of the 16S (targeting prokaryotes) and the ITS1 (targeting fungi) loci in the invaded oasis and tanks pre-removal were, however, successful in discerning a suite of 90 crayfish-associated taxa to serve as candidate bioindicators of invasive presence. Ranking these 90 taxa by their geographic distribution in eDNA surveys and by evidence of crayfish-associations in the literature, we support 9 taxa to be high-ranking, and suggest they be prioritized in future biomonitoring. Biodiversity analyses from five metabarcode loci including plants, animals, and both prokaryotic and eukaryotic microbes showed that communities differed but that species richness remained relatively similar between oases through time. Our results reveal that, while there are limitations of eDNA approaches to detect crayfish and other invasive species, microbial bioindicators offer a largely untapped biomonitoring opportunity for invasive species management, adding a valuable resource to a conservation manager’s toolkit.

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Effects of salinity, stocking density and feeding in Macrobrachium pantanalense larviculture

Freitas

Hayd

Calado

2021

Aquaculture Reports

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Cypermethrin-based formulation Barrage® induces histological changes in gills of the Pantanal endemic shrimp Macrobrachium pantanalense

Cited by 9 publications

References 30 publications

Environmental DNA reveals invasive crayfish microbial associates and ecosystem‐wide biodiversity before and after eradication

Environmental DNA reveals invasive crayfish microbial associates and ecosystem‐wide biodiversity before and after eradication

Environmental DNA reveals invasive crayfish microbial associates and ecosystem-wide biodiversity before and after eradication

Effects of salinity, stocking density and feeding in Macrobrachium pantanalense larviculture

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