CYP1A Induction and Human Risk Assessment: An Evolving Tale of in Vitro and in Vivo Studies: TABLE 1

Ma, Qiang; Lu, Anthony Y. H.

doi:10.1124/dmd.107.015826

Cited by 204 publications

(159 citation statements)

References 51 publications

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“…4-ABP did not induce CYP1A1, COX-2 or UGT1 up to concentrations of 50 M. In contrast to 4-ABP, B[a]P is known to be a strong inducer of CYP1A1 which acts via the Ah receptor (Ma and Lu 2007). In agreement with the latter studies we also observed an induction of the Ah receptor-dependent genes CYP1A1, COX-2 and UGT1 by B[a]P. Interestingly, induction of these genes by B[a]P was enhanced by coexposure to 4-ABP.…”

Section: Discussionmentioning

confidence: 92%

“…In this study, we analysed RNA expression of CYP1A1, COX-2 and UGT1, which are known to be induced via the Ah receptor (Ma and Lu 2007;Kawajiri and Fujii-Kuriyama 2007;Yang and Bleich 2004;Degner et al 2007;Zhou et al 2005;Mackenzie et al 2003). 4-ABP did not induce CYP1A1, COX-2 or UGT1 up to concentrations of 50 M. In contrast to 4-ABP, B[a]P is known to be a strong inducer of CYP1A1 which acts via the Ah receptor (Ma and Lu 2007).…”

Section: Discussionmentioning

confidence: 99%

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Synergism of aromatic amines and benzo[a]pyrene in induction of Ah receptor-dependent genes

et al. 2008

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Aromatic amines have been shown to cause bladder cancer. However, epithelial cells of the urinary bladder, cells of origin of bladder cancer, may be exposed to numerous substances besides aromatic amines. In the present study, we analysed possible interactions between the aromatic amines 4-aminobiphenyl (4-ABP) as well as 2-naphthylamine (2-NA) and the polycyclic aromatic hydrocarbon benzo[a]pyrene (B[a]P). For this purpose we incubated primary porcine urinary bladder epithelial cells (PUBEC) with concentrations of 1 to 50 M 4-ABP with and without coexposure to B[a]P. As expected B[a]P increased mRNA expression of cytochrome P450 1A1 (CYP1A1), whereas 4-ABP had no eVect. However, when co-exposed 4-ABP enhanced the induction of CYP1A1 by B[a]P. This result was conWrmed by Western blot analysis of CYP1A1 protein expression. A similar eVect as for CYP1A1 was also observed for cyclooxygenase-2 (COX-2) and UDP-glucuronosyltransferase 1 (UGT1). Next, we studied co-exposures of 2-NA and B[a]P. Similar as for 4-ABP also 2-NA enhanced B[a]P-mediated induction of CYP1A1. Our results demonstrate that some aromatic amines may enhance the inXuence of B[a]P on Ah receptor-dependent genes.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Synergism of aromatic amines and benzo[a]pyrene in induction of Ah receptor-dependent genes

et al. 2008

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“…Genes from the cytochrome P450 family encode enzymes involved in the oxidation of a variety of compounds; among these, CYP1A1 and CYP 1A2 play critical roles in the metabolic activation of carcinogenic HCA to electrophilic reactive intermediates, leading to toxicity and cancer [24]. A signifi cant association between CYP1A1, CYP1A2, and the risk of stomach cancer has been reported, as has the interaction of CYP1A1 polymorphisms and smoking in this cancer [25].…”

Section: Discussionmentioning

confidence: 99%

Association between dietary heterocyclic amine levels, genetic polymorphisms of NAT2, CYP1A1, and CYP1A2 and risk of stomach cancer: a hospital-based case-control study in Japan

Kobayashi

Otani²,

Iwasaki³

et al. 2009

Gastric Cancer

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“…As expected, caffeine treatment induces CYP1A2 expression in liver (Chen et al, 1996;Goasduff et al, 1996) but there is no evidence about the mechanism by which this enzyme expression is increased. Aryl-Hydrocarbon Receptor (AHR) is one of the receptors that regulate CYP1A family expression by binding of its specific ligands (Ma, Lu, 2007). Nonetheless, attempts failed to demonstrate that caffeine-induced CYP1A expression is due to AHR activation (Ayalogu et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Caffeine increases Nr1i3 expression and potentiates the effects of its ligand, TCPOBOP, in mice liver

Fukumasu

Rochetti

Latorre

et al. 2015

Braz. J. Pharm. Sci.

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Caffeine is one of the world's most consumed substances. It is present in coffee, green tea and guarana, among others. The xenobiotic-sensing nuclear receptor subfamily 1, group I, member 3 (Nr1i3), also known as the Constitutive Androstane Receptor (Car) is a key regulator of drug metabolism and excretion. No consistent description of caffeine effects on this receptor has been described. Thus, to unravel the effects of caffeine on this receptor, we performed experiments in mice. First, C57Bl/6 mice that were treated daily with caffeine (50 mg/kg) for 15 days presented a slight but significant increase in Nr1i3 and Cyp2b10 gene expression. A second experiment was then performed to verify the effects of caffeine on TCPOBOP (1,4-bis-[2-(3,5-dichloropyridyloxy)]benzene, 3,3′,5,5′-tetrachloro-1,4-bis(pyridyloxy) benzene), the most potent agonist known for mice Nr1i3. Interestingly, caffeine potentiated TCPOBOP pleiotropic effects in mice liver, such as hepatomegaly, hepatotoxicity, hepatocyte proliferation and loss of cell-to-cell communication through gap junctions. In addition, caffeine plus TCPOBOP treatment increased liver gene expression of Nr1i3 and Cyp2b10 comparing with only caffeine or TCPOBOP treatments. Together, these results indicate that caffeine increases the expression of Nr1i3 in mice liver, although at this point it is not possible to determine if Nr1i3 directly or indirectly mediates this effect.Uniterms: Caffeine/effects/experimental study. Androstane constitutive receptor/caffeine effects. 1,4-bis-[2-(3,5-dichloropyridiloxy)]benzeno,3,3′,5,5-tetracloro-1,4-bis(pyridiloxy)benzene/caffeine effects. Cytochrome p450.A cafeína é uma das substâncias mais consumidas mundialmente, estando presente no café, chá-verde e guaraná, entre outros. O receptor sensor de xenobióticos Receptor Nuclear subfamília 1, grupo I, membro 3 (Nr1i3, mais conhecido como Androstano Consititutivo -Car) é um regulador chave da biotransformação e excreção de substâncias e nenhuma descrição consistente dos efeitos da cafeína sobre este receptor foi feita. Então, para avaliar os efeitos da cafeína sobre este receptor, realizamos experimentos em camundongos. Primeiramente, camundongos C57/Bl/6 foram tratados diariamente com cafeína (50 mg/kg) por 15 dias e apresentaram um leve, mas significativo, aumento na expressão do Car e do seu gene alvo Cyp2b10. Assim, um segundo experimento foi realizado para verificar os efeitos da cafeína sobre o TCPOBOP (1,4-bis-[2-(3,5-dicloropiridiloxi)]benzeno,3,3′,5,5′-tetracloro-1,4-bis(piridiloxi)benzeno), o mais potente agonista do Nr1i3 de camundongos conhecido. Interessantemente, a cafeína potencializou os efeitos pleiotrópicos do TCPOBOP no fígado dos camundongos, como hepatomegalia, hepatotoxicidade, proliferação celular e perda da comunicação intercelular por junções do tipo gap. Os camundongos tratados com cafeína e TCPOBOP apresentaram maior expressão gênica de Nr1i3 e Cyp2b10, quando comparados aos camundongos tratados apenas com cafeína ou TCPOBOP. Juntos, nossos resultados indicam que a ...

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CYP1A Induction and Human Risk Assessment: An Evolving Tale of in Vitro and in Vivo Studies: TABLE 1

Abstract: ABSTRACT:CYP1A1 and 1A2 play critical roles in the metabolic activation of carcinogenic polycyclic aromatic hydrocarbons (PAHs) and heterocyclic aromatic amines/amides (HAAs), respectively, to electrophilic reactive intermediates, leading to toxicity and cancer.

Cited by 204 publications

References 51 publications

Synergism of aromatic amines and benzo[a]pyrene in induction of Ah receptor-dependent genes

Synergism of aromatic amines and benzo[a]pyrene in induction of Ah receptor-dependent genes

Association between dietary heterocyclic amine levels, genetic polymorphisms of NAT2, CYP1A1, and CYP1A2 and risk of stomach cancer: a hospital-based case-control study in Japan

Caffeine increases Nr1i3 expression and potentiates the effects of its ligand, TCPOBOP, in mice liver

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