Cymbopogon citratus protect against lead-induced suppression of haematological and tubuloglomerular functions as well as disruption of hepatocellular membranes in male Wistar rats

Eruotor, Harrison Ogheneochuko; Asiwe, Jerome Ndudi; Eruotor, Taiye Martha

doi:10.1016/j.jtemin.2022.100045

Cited by 3 publications

(3 citation statements)

References 31 publications

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“…Membrane properties Osmotic fragility Spectrophotometry [85] Morphology SEM, flow cytometry [11,76] Membrane lipid peroxidation MDA level, mass spectrometry [89,90] Membrane stiffness AFM [91] PS exposure and CD47 expression Flow cytometry [92] Protein activities Hb content HiCN, proteomics, and metabolomics [93,94] Band-3 phosphorylation Immunofluorescence staining, proteomics, and metabolomics [95,96] ATPase activity a Inorganic phosphorus method [54] Protein oxidation Carbonylation assay [89] Antioxidant enzyme activity SOD: xanthine oxidase method [97] CAT: ammonium molybdate colorimetry membrane quality after cryopreservation. Flow cytometry analysis of RBC PS externalization showed that freezing greatly damaged the membrane asymmetry of both l-RBCs (liposome-treated RBCs) and c-RBCs (control RBCs, not treated by liposomes).…”

Section: Properties Of Rbcs Parameters Detection Methods Referencesmentioning

confidence: 99%

“…Hb can be estimated by the hemoglobincyanide method (HiCN). The number of RBCs per milliliter is determined using a hemocytometer 93,119 . Consequently, the Hb content of RBCs could be obtained.…”

Section: The Effectiveness Of Cpasmentioning

confidence: 99%

“…The number of RBCs per milliliter is determined using a hemocytometer. 93,119 Consequently, the Hb content of RBCs could be obtained. Liu et al 42 explored the potential of tricine as a CPA for RBC cryopreservation.…”

Section: Hb Contentmentioning

confidence: 99%

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Cryoprotectants for red blood cells: Evaluate safety and effectiveness by in vitro measures

Hu,

Liu,

Ahmad

et al. 2023

MedComm – Future Medicine

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The transfusion of red blood cells (RBCs) is crucial in current medicinal research. The shelf‐life of donated RBCs preserved by the normal method is very short, limiting their clinical application. Cryopreservation is a reliable and effective technology for the long‐term storage of RBCs. During the process, ice formation will cause irreversible injuries to RBCs. Glycerol is used as a cryoprotectant (CPA) for RBCs to mitigate cryoinjuries. But it severely induces RBC hemolysis and deformation. This review introduces some biocompatible and effective CPAs used for RBC cryopreservation, outlining recent advances. Currently, there is no uniform standard to determine whether a CPA is suitable for RBCs. According to previous studies, we summarize for the first time comprehensive methods to evaluate the performance of CPAs by ensuring their safety and effectiveness. The safety of CPAs is defined as the degree of damage to RBCs, while the effectiveness is demonstrated by the properties of thawed RBCs, including membrane properties, protein activities, rheological properties, and metabolites levels. A novel CPA that is confirmed suitable for RBCs by these methods may be applied to other cells. We believe comprehensive methods can thoroughly evaluate the performance of CPAs and promote the development of transfusion therapy in clinic.

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