Cymbidium: Successful in Vitro Growth and Subsequent Acclimatization

Silva, J.A. Teixeira da; Norikane, Atsushi; Tanaka, Masanobu

doi:10.17660/actahortic.2007.748.27

Cited by 13 publications

(3 citation statements)

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“…Induced seed germination can be asymbiotic under in vitro conditions in a special culture medium or using symbiotic methods, which combines seed germination with the presence of a symbiont fungus [53]. Seed germination and propagation by symbiotic approaches are the preferred options for Cymbidiums' reintroduction into native habitats [52].…”

Section: Asymbiotic Seed Germination In Cymbidiumsmentioning

confidence: 99%

“…On the other hand, in vitro cultivation methods or asymbiotic orchid germination provides an interesting platform for the large-scale propagation of orchids without the requisite of fungal symbiosis. These methods facilitate the seeding and cultivation of seedlings on a different culture medium formulation, basically containing nutrients, carbohydrate sources, and agar, cultivated under aseptically controlled conditions [53].…”

Section: Asymbiotic Seed Germination In Cymbidiumsmentioning

confidence: 99%

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Medicinal Use, Flower Trade, Preservation and Mass Propagation Techniques of Cymbidium Orchids—An Overview

Balilashaki,

Martinez-Montero,

Vahedi

et al. 2023

Horticulturae

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Cymbidium is an economically important genus in the orchid family (Orchidaceae) that has a pronounced medicinal and ornamental value. Medicinally, the plant is employed as a tonic to treat weakness in chronic diseases, dizziness, eye problems, burns, and wounds, etc. Cymbidiums are highly prized for their graceful flowers and sweet fragrance and are among the top ten most popular cut flowers. They are one of the most important commercial orchid groups and account for 3% of cut flowers in floriculture. Some orchid species in this genus are particularly threatened by excessive harvesting, so conservation measures are needed. Several enthusiastic organizations (e.g., The Cymbidiums Society of America, The Cymbidiums Club in Australia, The Golden Gate Cymbidiums Society, Alameda, CA, etc.) are dedicated to propagating, conserving, promoting, appreciating, and disseminating information about these beautiful and charming orchids. Through organogenesis (direct and indirect) and somatic embryogenesis, extensive propagation techniques for Cymbidiums have been developed to create protocols for synthetic seed production leading to large-scale propagation and long-term ex situ and in vitro conservation. This review highlights the medicinal uses, flower trade, conservation, and massive propagation techniques of Cymbidium orchids.

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Section: Asymbiotic Seed Germination In Cymbidiumsmentioning

confidence: 99%

Section: Asymbiotic Seed Germination In Cymbidiumsmentioning

confidence: 99%

Medicinal Use, Flower Trade, Preservation and Mass Propagation Techniques of Cymbidium Orchids—An Overview

Balilashaki,

Martinez-Montero,

Vahedi

et al. 2023

Horticulturae

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“…PLBs, which are transitional structures connecting embryo and plant were found to be outstanding explants for accelerated propagation in vitro. In addition, the cells of PLB are highly meristematic in nature, thus successfully used to enhance proliferation and production of several ornamental plants like Zygopetalum intermedium (Nagaraju and Mani 2005) Cymbidium hybrid (Teixeira da Silva et al 2007), and Cymbidium giganteum (Hossain et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Direct induction of protocorm-like bodies from shoot tips, plantlet formation, and clonal fidelity analysis in Anthurium andreanum cv. CanCan

et al. 2012

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Protocorm-like bodies (PLBs) were induced directly at high frequency from wounded surface of Anthurium andreanum cv. CanCan shoot tip-ends, used as explants. In order to obtain PLB directly, the influence of different types and concentrations of cytokinins were evaluated. Amid the cytokinins, N 6 -(D 2 -isopentenyl)-adenine (2-iP) at a concentration of 15 lM was most effective in inducing PLB whereby *98 (97.8) % of explants induced PLB with an average of 120 PLBs per shoot tip within 50 days of culture. Stereomicroscopic observation meticulously revealed the sequential changes from initiation to maturation of PLB gradually forming shoot apical meristem, shoot primordia and leaf primordia. Mature PLBs showed significant shoot proliferation (98.4 %) in media containing 10 lM 6-furfurylaminopurine forming 17 shoots per PLB within 30 days. The inclusion of activated charcoal (AC) in media containing auxin had promotive effect on rooting whereby 5 lM indole-3-butyric acid plus 500 lM AC resulted in highest number and length of roots. Successfully acclimatized plants, subjected to random amplified polymorphic DNA assessment for genetic fidelity, did not show any variation. Thus, this complete study has successfully outlined a rapid, high frequency direct induction of PLB of Anthurium from shoot tips inclusive of shoot proliferation, rooting and acclimatization.

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