Thin Cell Layers: The Technique

Silva, Jaime A. Teixeira da; Tanaka, Michio; Davey, M. R.; Anthony, Paul

doi:10.1002/9780470686522.ch2

Cited by 5 publications

(1 citation statement)

References 16 publications

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“…Since then, this technique has been successfully applied in the micropropagation of various taxa including vegetable crops, legumes, ornaments and medicinal plants (Abdolinejad et al 2020;Hieu et al 2018;Nhut et al 2003a;2003b;Teixeira da Silva and Nhut 2003;Sudhakaran et al 2006;Taylor and Van Staden 2006;Leguillon et al 2003;Ghnaya et al 2008;Swarna and Ravindharan 2013). The technique of TCL culture has the advantage over traditional tissue culture methods in terms of overall plantlet production, minimal use of explant tissue and sensitivity and responsiveness to the substances added to the culture medium due to the close contact with the growth medium and faster diffusion of nutrients and PGRs as the explant size is smaller (Lakshmanan et al 1995;Teixeira da Silva and Tanaka 2011). Therefore, the objective of our study was to develop an e cient and reliable protocol for micropropagation of C. punctatum by using tTCL explants and the successful establishment of micropropagated plants in eld conditions.…”

Section: Introductionmentioning

confidence: 99%

Transverse thin cell layer (tTCL) technology: a promising tool for micropropagation of Centratherum punctatum Cass.

Aswathi

Thomas

2023

In Vitro Cell.Dev.Biol.-Plant

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Centratherum punctatum Cass. is an important medicinal herb used for various purposes. The present study describes an e cient protocol for the micropropagation of C. punctatum through transverse Thin Cell Layers (tTCLs). The effect of plant growth regulators (PGRs), thickness of tTCLs and source of tTCLs, were evaluated. The tTCLs of varying thickness (0.5-5.0 mm) were excised from 10-day-old leaf, 45-day-old node and internode and cultured on MS medium supplemented with various concentrations of PGRs (BAP, TDZ, KN; 0.1-2.0 mg/l) alone or in combination with NAA (0.1-1.0 mg/l) for shoot induction.For callus induction 2, 4-D (0.5-2.0 mg/l) was employed. The leaf and node tTCLs showed direct shoot regeneration and internodal tTCLs resulted in calli which successfully regenerated to shoots on subculture. A signi cant effect of thickness/width on shoot induction was observed. For leaf, 1.0 mm width and for node and internode 2.0 mm thick tTCLs showed maximum response. MS medium supplemented with 1.5 mg/l TDZ, 0.5 mg/l TDZ and 1.0 mg/l BAP in combination with 0.2 mg/l NAA were found optimum for shoot induction from tTCLs of leaf (96% response with 41.5 shoots per explant), internode (64% response with 22.9 shoots per explant) and node (87% response with 11.9 shoots per explant) respectively. Rhizogenesis was obtained when micropropagated shoots were transferred to half strength MS medium supplemented with various concentrations (0.25-2.0 mg/l) of IBA. The rooted plantlets were eventually acclimatized and transferred to soil. The procedure described here is a promising tool for micropropagation of C. punctatum as it produces high frequency healthy shoots from minimum explant source. Key MessageA rapid and e cient micropropagation protocol has been standardized in C. punctatum through transverse thin cell layer (tTCL) explants from leaf, node and internode.

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Section: Introductionmentioning

confidence: 99%

Transverse thin cell layer (tTCL) technology: a promising tool for micropropagation of Centratherum punctatum Cass.

Aswathi

Thomas

2023

In Vitro Cell.Dev.Biol.-Plant

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Thin Cell Layers: Power-Tool for Organogenesis of Floricultural Crops

Silva

2009

Protocols for in Vitro Propagation of Ornamental Plants

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Thin cell layers or TCLs have been a fundamental corner-stone of tissue culture of all plant species, including floricultural and ornamental plants (FOPs). In this review, the current status of the use of TCL technology to specific FOPs will be outlined while certain successes and difficulties will be focused. The ability to control developmental and morphogenetic processes in vitro through the use of TCLs, especially where conventional methods have not always produced ideal results, is the key in taking lab-based theory to business-based applications. TCL technology, which focuses more on the size rather than the origin of the explant, is a gateway for FOP regeneration and transformation studies. Some general principles and guidelines for quality verification will be provided.

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Cellular Totipotency

Bhojwani

Dantu

2013

Plant Tissue Culture: An Introductory Text

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Thin Cell Layers: The Technique

Cited by 5 publications

References 16 publications

Transverse thin cell layer (tTCL) technology: a promising tool for micropropagation of Centratherum punctatum Cass.

Transverse thin cell layer (tTCL) technology: a promising tool for micropropagation of Centratherum punctatum Cass.

Thin Cell Layers: Power-Tool for Organogenesis of Floricultural Crops

Cellular Totipotency

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