Cyclopiazonic acid and aflatoxins production byAspergillus flavus isolated from Argentinian corn

Resnik, Silvia L.; González, Horacio F.; Pacin, A.; Viora, M.; Caballero, Gerardo; Gros, Eduardo G.

doi:10.1007/bf03192263

Cited by 21 publications

(11 citation statements)

References 17 publications

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“…For testing aflatoxin B 1 (AFB 1 ), aflatoxin B 2 (AFB 2 ), aflatoxin G 1 (AFG 1 ), and aflatoxin G 2 (AFG 2 ), rice media was inoculated with 50 μl of the conidial suspension of A. flavus and incubated for 1 week, at 25°C (Resnik et al 1996, Moltó et al 1997. Rice medium was prepared with distilled water added with up to 40 % of polished rice in 250 ml Erlenmeyers, and autoclaved at 121°C for 30 min.…”

Section: Strains Culture Conditions and Mycotoxin Inductionmentioning

confidence: 99%

Mycotoxigenic potential of fungi isolated from freshly harvested Argentinean blueberries

et al. 2014

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Alternaria alternata, A. tenuissima, Fusarium graminearum, F. semitectum, F. verticillioides, Aspergillus flavus, and Aspergillus section Nigri strains obtained from blueberries during the 2009 and 2010 harvest season from Entre Ríos, Argentina were analyzed to determine their mycotoxigenic potential. Taxonomy status at the specific level was determined both on morphological and molecular grounds. Alternariol (AOH), alternariol monomethyl ether (AME), aflatoxins (AFs), zearalenone (ZEA), fumonisins (FBs), and ochratoxin A (OTA) were analyzed by HPLC and the trichotecenes deoxynivalenol (DON), nivalenol (NIV), HT-2 toxin (HT-2), T-2 toxin (T-2), fusarenone X (FUS-X), 3-acetyl-deoxynivalenol (3-AcDON), and 15-acetyl-deoxynivalenol (15-AcDON) by GC. Twenty-five out of forty two strains were able to produce some of the mycotoxins analyzed. Fifteen strains of Aspergillus section Nigri were capable of producing Fumonisin B1 (FB1); two of them also produced Fumonisin B2 (FB2) and one Fumonisin B3 (FB3). One of the F. graminearum isolated produced ZEA, HT-2, and T-2 and the other one was capable of producing ZEA and DON. Two A. alternata isolates produced AOH and AME. Four A. tenuissima were capable of producing AOH and three of them produced AME as well. One Aspergillu flavus strain produced aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), and aflatoxin G1 (AFG1). To our knowledge, this is the first report showing mycotoxigenic capacity of fungal species isolated from blueberries that include other fungi than Alternaria spp.

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Section: Strains Culture Conditions and Mycotoxin Inductionmentioning

confidence: 99%

Mycotoxigenic potential of fungi isolated from freshly harvested Argentinean blueberries

et al. 2014

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“…Different results were reported by Nesci and Etcheverry (2002) from preplanting maize soils, who reported a high percentage (82%) of nontoxigenic A. flavus. There is little data available on CPA potential producer A. flavus strains (Blaney et al 1989;Resnik et al 1996;Barros et al 2005). However, it is evident that a high percentage of the strains are potential producers of this mycotoxin.…”

Section: Aspergillus Section Flavi Toxigenic Capacitymentioning

confidence: 99%

Survey of Aspergillus section Flavi presence in agricultural soils and effect of glyphosate on nontoxigenic A. flavus growth on soil-based medium

et al. 2014

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Aims: To evaluate the cultivable mycobiota from agricultural soils exposed to pesticides, the aflatoxigenic capacity of Aspergillus section Flavi strains and the effect of glyphosate on lag phase and growth rates of native nontoxigenic Aspergillus flavus under different water potential (MPa) conditions on soilbased medium. Methods and Results: Culturable mycobiota analysis from different agricultural soils was performed by the surface spread method. The effect of glyphosate (0-20 mmol l À1 ) on the growth of A. flavus strains was evaluated on a soil extract solid medium. Mycobiota analysis of crop soils showed the presence of twenty-one genera of filamentous fungi. Aspergillus flavus and Aspergillus niger aggregate strains were isolated from the three soil types. Ninety-two per cent of A. flavus strains were toxigenic. In vitro assay results showed that at À0Á70 MPa, a significant increase in growth rate in all strains was recorded at 5 and 20 mmol l À1 of glyphosate. At À2Á78 MPa, this parameter remained constant at all glyphosate concentrations, except in GM4 strain where an increase in growth rate was recorded with increasing pesticide concentrations. At À7Á06 MPa, a significant increase in growth rate has also been observed in GM 3 strain with 5 mmol l À1 and in GM 4 strain with 10 and 20 mmol l À1 . Conclusions: This study showed that the imperfecti fungi Aspergillus spp., Penicillium spp., Trichoderma spp., Cladosporium spp. and Paecilomyces spp. are isolated as prevalent groups in agricultural soil exposed to pesticides, and the capacity of nontoxigenic A. flavus strains to tolerate different glyphosate concentrations under different water potential (MPa) conditions. Significance and Impact of the Study: This manuscript makes a contribution to the knowledge of cultivable fungal populations from agricultural soils exposed to pesticides and the glyphosate tolerance of A. flavus strains.

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“…The frequency of detection was 60% for the Caiapó, with mean levels ranging from 304.1 to 2583.7 μg/kg, and 74.3% for the 886, with levels ranging from 288.0 to 4918.1 μg/kg [17]. Other studies investigating the production of CPA in peanuts also reported high rates of 89 [52], 93 [53], and 97% [54]. Alatoxins and CPA were also detected simultaneously in kernel samples (11.4%).…”

Section: Resultsmentioning

confidence: 85%

First Report of Mycotoxins in Second Peanuts Crop in Adana and Osmaniye at Harvest, Drying, Prestorage and Storage Periods

Lavkor¹,

Var²,

Öztemiz³

et al. 2017

Aflatoxin-Control, Analysis, Detection and Health Risks

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Alatoxin (AF) and cyclopiazonic acid (CPA) contaminations are very important problems for peanuts and its products. The aim of the study was to detect alatoxin (types B and G) and cyclopiazonic acid (CPA) occurrence and critical periods of toxin production in peanuts collected from diferent research areas of Osmaniye and Adana, Turkey, in 2015. Peanut kernels toxin analysis was performed in four diferent periods during the harvest, drying, prestorage, and storage. Total alatoxin occurrence in peanut kernels was analyzed by immunoainity chromatography-reversed-phase high-performance liquid chromatography (IAC-HPLC) analysis and cyclopiazonic acid occurrence in peanut kernels was analyzed by thin layer chromatography (TLC). Alatoxin levels in 76 out of 102 contaminated samples were from 0.3 to 1333.42 μg/kg. Cyclopiazonic acid levels in 18 out of 102 peanut samples were from 16.6 to 44.44 μg/kg. An unusual patern of mycotoxin production (alatoxin types B and G simultaneously with CPA) was seen in 11 of 102 peanuts samples. Six of nine samples were from the storage period. Alatoxin contamination during harvesting (64%) and drying (75%) were higher than prestorage (53%). Alatoxin (93%) and cyclopiazonic acid (30%) were the most produced during storage. The results showed that storage period was signiicantly important for the presence of two mycotoxins according to the statistical analysis.

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Cyclopiazonic acid and aflatoxins production byAspergillus flavus isolated from Argentinian corn

Cited by 21 publications

References 17 publications

Mycotoxigenic potential of fungi isolated from freshly harvested Argentinean blueberries

Mycotoxigenic potential of fungi isolated from freshly harvested Argentinean blueberries

Survey of Aspergillus section Flavi presence in agricultural soils and effect of glyphosate on nontoxigenic A. flavus growth on soil-based medium

First Report of Mycotoxins in Second Peanuts Crop in Adana and Osmaniye at Harvest, Drying, Prestorage and Storage Periods

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