Cyclooxygenase-2 mediated synergistic effect of ursolic acid in combination with paclitaxel against human gastric carcinoma

Xu, Xiaogang; Zhu, Guofu; Zhang, Kai; Zhou, Yichan; Li, Xin; Xu, Wei; Zhang, Hao; Shao, Yun; Zhang, Zhenyu; Sun, Wei

doi:10.18632/oncotarget.21576

Cited by 17 publications

(14 citation statements)

References 40 publications

(54 reference statements)

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“…The miRNA-targeted mRNA was predicted through miRDB, miRTarBase, and TargetScan ( Table 4 ). Only 2 differentially expressed mRNAs in the ceRNA network, PTGS2 and DUSP2, have previously been reported as tumor suppressor genes [ 10 , 12 – 14 ] ( Figure 3 ). In the ceRNA network, 12 lncRNAs, 2 miRNAs, and 2 mRNAs are downregulated, while 12 lncRNAs and 4 miRNAs are upregulated ( Table 5 , Figure 4 ).…”

Section: Resultsmentioning

confidence: 99%

Identification of Potential Prostate Cancer-Related Pseudogenes Based on Competitive Endogenous RNA Network Hypothesis

Jiang

Guo

et al. 2018

Med Sci Monit

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BackgroundLong noncoding RNAs (lncRNAs) have been revealed to function as competing endogenous RNAs (ceRNAs), which can seclude the common microRNAs (miRNAs) and hence prevent the miRNAs from binding to their ancestral gene. Nonetheless, the role of lncRNA-mediated ceRNAs in prostate cancer has not yet been elucidated.Material/MethodsUsing The Cancer Genome Atlas (TCGA) database, lncRNA, miRNA, and mRNA profiles from 499 prostate cancer tissues and 52 normal prostate tissues were analyzed with the R package “DESeq” to identify the differentially expressed RNAs. GO and KEGG pathway analyses were performed using “DAVID6.8” and R packages “Clusterprofile.” The ceRNA network in prostate cancer was constructed using miRDB, miRTarBase, and TargetScan databases. Survival analysis was performed with Kaplan-Meier analysis.ResultsA total of 376 lncRNAs, 33 miRNAs, and 687 mRNAs were identified as significant factors in tumorigenesis. Based on the hypothesis that the ceRNA network (lncRNA-miRNA-mRNA regulatory axis) is involved in prostate cancer and forms competitive interrelations between miRNA and mRNA or lncRNA, we constructed a ceRNA network that included 23 lncRNAs, 6 miRNAs, and 2 mRNAs that were differentially expressed in prostate cancer. Only 3 lncRNAs (LINC00308, LINC00355, and OSTN-AS1) had a significant association with survival (P<0.05). The 3 prostate cancer-specific lncRNA were validated in prostate cancer cell lines PC3 and DU145 using qRT-PCR.ConclusionsWe demonstrated the differential lncRNA expression profiles in prostate cancer, which provides new insights for future studies of the ceRNA network and its regulatory mechanisms in prostate cancer.

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Section: Resultsmentioning

confidence: 99%

Identification of Potential Prostate Cancer-Related Pseudogenes Based on Competitive Endogenous RNA Network Hypothesis

Jiang

Guo

et al. 2018

Med Sci Monit

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“…For example, UA inhibits the nuclear translocation of β‐catenin, upregulates the expression of p53, and induces apoptosis in osteosarcoma cells in vitro and in vivo . UA can also enhance paclitaxel‐induced apoptosis by upregulating cyclooxygenase‐2, proliferating cell nuclear antigen (PCNA), and Bcl‐2 in gastric cancer cells . Besides of the action on intracellular signaling, Sohn et al showed that UA induced apoptosis via inhibition of epithelial mesenchymal transition (EMT) and activation of let7b in mesothelioma cells .…”

Section: Introductionmentioning

confidence: 99%

“…15 UA can also enhance paclitaxel-induced apoptosis by upregulating cyclooxygenase-2, proliferating cell nuclear antigen (PCNA), and Bcl-2 in gastric cancer cells. 18 Besides of the action on intracellular signaling, Sohn et al showed that UA induced apoptosis via inhibition of epithelial mesenchymal transition (EMT) and activation of let7b in mesothelioma cells. 19 In addition, UA induces autophagy by triggering endoplasmic reticulum (ER) stress and reactive oxygen species (ROS) in glioma cells.…”

Section: Introductionmentioning

confidence: 99%

Ursolic acid induces apoptosis and autophagy in oral cancer cells

Lin

Chin

Lee

et al. 2019

Environmental Toxicology

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Oral squamous cell carcinoma (OSCC) is the fifth common cause of cancer mortality in Taiwan with high incidence and recurrence and needs new therapeutic strategies. In this study, ursolic acid (UA), a triterpenoid, was examined the antitumor potency in OSCC cells. Our results showed that UA inhibited the proliferation of OSCC cells in a dose‐ and time‐dependent manner in both Ca922 and SCC2095 oral cancer cells. UA induced caspase‐dependent apoptosis accompanied with the modulation of various biological biomarkers including downregulating Akt/mTOR/NF‐κB signaling, ERK, and p38. In addition, UA inhibited angiogenesis as evidenced by abrogation of migration/invasion and blocking MMP‐2 secretion in Ca922 cells. Interestingly, UA induced autophagy in OSCC cells, as manifested by LC3B‐II conversion and increased p62 expression and accumulation of autophagosomes. Inhibition by autophagy inhibitor enhanced UA‐mediated apoptosis in Ca922 cells. The experiment provides a rationale for using triterpenoid in the treatment of OSCC.

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“…PTX is widely used as an anticancer drugs for various cancer including HNSCC, but has been associated with severe toxicity [26]. The combination of different chemotherapeutics is of great advantage; especially the herbal combination can provide reduced toxicity and enhance anticancer activity [27]. Here we report the liposomal co-delivery of PTX and UA with the aim to reduce the toxicity and increase the potency of PTX.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of clinical effectiveness of paclitaxel and ursolic acid co-loaded liposomes as enhanced treatment for head and neck squamous cell carcinoma

Lv¹,

Tan²,

Shang³

et al. 2019

Trop. J. Pharm Res

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Purpose: To enhance the clinical effectiveness of paclitaxel (PTX) by co-delivery with ursolic acid (UA) for the treatment of head and neck cancer Methods: Co-loaded liposomes of PTX and UA (UA-PTX-LiP) were prepared by thin-film hydration method. Their size and loading efficiency were determined using dynamic light scattering (DLS) technique and high performance liquid chromatography (HPLC), respectively. The effectiveness of UA-PTX-LiP against HSC-3 human head and neck cancer cell-lines was compared with that of PTX liposome (PTX-LiP) using systemic cell-based in vitro evaluation with MTT assay. Fluorescent microscopy was used for cell uptake studies. Results: The size of the prepared UA-PTX-LiP was 126.5 ± 3.22 nm. The ratiometric system for PTX and UA as liposomes revealed significantly enhanced cytotoxicity, with comparatively lower IC50, when compared to individual PTX-Lip. Fluorescent microscopy revealed the internalization ability of UA-PTX-LiP by targeted delivery of PTX in HSC-3 human head and neck cancer cell-line. Conclusion: These results show that UA-PTX-LiP successfully enhances the therapeutic potential and clinical outcomes of PTX in head-and-neck cancer, and also demonstrate the useful effect of combination of UA and PTX in chemotherapy.

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Cyclooxygenase-2 mediated synergistic effect of ursolic acid in combination with paclitaxel against human gastric carcinoma

Cited by 17 publications

References 40 publications

Identification of Potential Prostate Cancer-Related Pseudogenes Based on Competitive Endogenous RNA Network Hypothesis

Identification of Potential Prostate Cancer-Related Pseudogenes Based on Competitive Endogenous RNA Network Hypothesis

Ursolic acid induces apoptosis and autophagy in oral cancer cells

Evaluation of clinical effectiveness of paclitaxel and ursolic acid co-loaded liposomes as enhanced treatment for head and neck squamous cell carcinoma

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