Cyclical and Patch-Like GDNF Distribution along the Basal Surface of Sertoli Cells in Mouse and Hamster Testes

Satô, Takeshi; Aiyama, Yoshimi; Ishii-Inagaki, Mayuko; Hara, Kenshiro; Tsunekawa, Naoki; Harikae, Kyoko; Uemura-Kamata, Mami; Shinomura, Mai; Zhu, Xiao; Maeda, Seishi; Kuwahara-Otani, Sachi; Kudo, Akihiko; Kawakami, Hayato; Kanai‐Azuma, Masami; Fujiwara, Michio; Miyamae, Yoichi; Yoshida, Shosei; Seki, Makoto; Kurohmaru, Masamichi; Kanai, Yoshikatsu

doi:10.1371/journal.pone.0028367

Cited by 49 publications

(49 citation statements)

References 52 publications

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“…1C; Supplementary Figure S6, see section on supplementary data given at the end of this article). Anti-GFRa1 and anti-HuB immunostaining revealed that the GFRa1C undifferentiated spermatogonia (including spermatogenic stem cells; Sato et al 2011) and HuBC differentiated spermatogonia (including pre-leptotene spermatocytes; Supplementary Figure S7) are detectable in the basal compartment of the seminiferous tubules on day 4 after DT treatment. However, both spermatogonial populations were depleted by day 10 after DT treatment (Fig.…”

Section: Dt Injection Induces Sertoli Cell Degeneration In Postnatal mentioning

confidence: 99%

A novel Amh-Treck transgenic mouse line allows toxin-dependent loss of supporting cells in gonads

Shinomura¹,

Kishi²,

Tomita³

et al. 2014

Reproduction

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Cell ablation technology is useful for studying specific cell lineages in a developing organ in vivo. Herein, we established a novel anti-Mü llerian hormone (AMH)-toxin receptor-mediated cell knockout (Treck) mouse line, in which the diphtheria toxin (DT) receptor was specifically activated in Sertoli and granulosa cells in postnatal testes and ovaries respectively. In the postnatal testes of Amh-Treck transgenic (Tg) male mice, DT injection induced a specific loss of the Sertoli cells in a dose-dependent manner, as well as the specific degeneration of granulosa cells in the primary and secondary follicles caused by DT injection in Tg females. In the testes with depletion of Sertoli cell, germ cells appeared to survive for only several days after DT treatment and rapidly underwent cell degeneration, which led to the accumulation of a large amount of cell debris within the seminiferous tubules by day 10 after DT treatment. Transplantation of exogenous healthy Sertoli cells following DT treatment rescued the germ cell loss in the transplantation sites of the seminiferous epithelia, leading to a partial recovery of the spermatogenesis. These results provide not only in vivo evidence of the crucial role of Sertoli cells in the maintenance of germ cells, but also show that the Amh-Treck Tg line is a useful in vivo model of the function of the supporting cell lineage in developing mammalian gonads.

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Section: Dt Injection Induces Sertoli Cell Degeneration In Postnatal mentioning

confidence: 99%

A novel Amh-Treck transgenic mouse line allows toxin-dependent loss of supporting cells in gonads

Shinomura¹,

Kishi²,

Tomita³

et al. 2014

Reproduction

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“…5 The microenvironment of SSCs in the basal compartment of the seminiferous epithelium is important for the maintenance and self-renewal of these cells 6,7 because Sertoli cells provide the growth factors necessary for selfrenewal in this microenvironment. [8][9][10][11] Glial cell line-derived neurotrophic factor (GDNF) is the most crucial factor in the balance between self-renewal and differentiation in the SSC pool [12][13][14] as well as the promotion of SSCs' self-renewal. Without this factor, either spermatogonial aggregates do not develop or SSCs perish.…”

Section: Introductionmentioning

confidence: 99%

The effects of poly L-lactic acid nanofiber scaffold on mouse spermatogonial stem cell culture

Eslahi¹,

Hadjighassem²,

Joghataei³

et al. 2013

IJN

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Introduction A 3D-nanofiber scaffold acts in a similar way to the extracellular matrix (ECM)/basement membrane that enhances the proliferation and self-renewal of stem cells. The goal of the present study was to investigate the effects of a poly L-lactic acid (PLLA) nanofiber scaffold on frozen-thawed neonate mouse spermatogonial stem cells (SSCs) and testis tissues. Methods The isolated spermatogonial cells were divided into six culture groups: (1) fresh spermatogonial cells, (2) fresh spermatogonial cells seeded onto PLLA, (3) frozen-thawed spermatogonial cells, (4) frozen-thawed spermatogonial cells seeded onto PLLA, (5) spermatogonial cells obtained from frozen-thawed testis tissue, and (6) spermatogonial cells obtained from frozen-thawed testis tissue seeded onto PLLA. Spermatogonial cells and testis fragments were cryopreserved and cultured for 3 weeks. Cluster assay was performed during the culture. The presence of spermatogonial cells in the culture was determined by a reverse transcriptase polymerase chain reaction for spermatogonial markers ( Oct4, GFRα-1, PLZF, Mvh(VASA), Itgα6 , and Itgβ1 ), as well as the ultrastructural study of cell clusters and SSCs transplantation to a recipient azoospermic mouse. The significance of the data was analyzed using the repeated measures and analysis of variance. Results The findings indicated that the spermatogonial cells seeded on PLLA significantly increased in vitro spermatogonial cell cluster formations in comparison with the control groups (culture of SSCs not seeded on PLLA) ( P ≤0.001). The viability rate for the frozen cells after thawing was 63.00% ± 3.56%. This number decreased significantly (40.00% ± 0.82%) in spermatogonial cells obtained from the frozen-thawed testis tissue. Both groups, however, showed in vitro cluster formation. Although the expression of spermatogonial markers was maintained after 3 weeks of culture, there was a significant downregulation for some spermatogonial genes in the experimental groups compared with those of the control groups. Furthermore, transplantation assay and transmission electron microscopy studies suggested the presence of SSCs among the cultured cells. Conclusion Although PLLA can increase the in vitro cluster formation of neonate fresh and frozen-thawed spermatogonial cells, it may also cause them to differentiate during cultivation. The study therefore has implications for SSCs proliferation and germ cell differentiation in vitro.

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“…Perhaps, a recommended way to reach these goals is culture of stem cells in the presence of growth factors. Among the growth factors, GDNF (Glial-Cell-Line-Derived Neurotrophic Factor) is the most crucial factor to promote SSC self-renewal leading to a balance set between SSCs self-renewal and differentiation [47][48][49]. Without GDNF, spermatogonial aggregation cannot occur or SSCs may be perished [50].…”

Section: Discussionmentioning

confidence: 99%

Alteration of spermatogenesis following spermatogonial stem cells transplantation in testicular torsion-detorsion mice

Azizollahi

Aflatoonian

Gilani

et al. 2016

J Assist Reprod Genet

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Purpose Testicular ischemia is the main consequence of testicular torsion, in both clinical and experimental aspects. Preservation and auto-transplantation of spermatogonial stem cells (SSCs) could be a new treatment for infertility in testicular ischemia following testicular torsion. Methods To apply the idea in this study, animals were randomly divided into four groups of control, sham, with torsion, and with torsion followed by transplantation (TT). Isolated SSCs from neonatal mice were cultured and identified by flow cytometry (C-KIT − , INTEGRIN β 1 + ) and RT-PCR (Reverse transcription polymerase chain reaction) for specific spermatogonial cell markers (Oct4, Gfrα-1, Plzf, Vasa, Itgα 6 , and Itgβ 1 ). SSCs were transplanted upon a 2-h testicular torsion in the TT group. Cultured cells were transplanted into ischemia reperfusion testicle 2 weeks post-testicular torsion. Eight weeks after SSCs transplantation, the SSCs-transplanted testes and epididymis were removed for sperm analysis, weight and histopathological evaluation, and pre-and postmeiotic gene expression assessment by qRT-PCR. Results Our findings indicated that all evaluated parameters (epididymal sperm profile, Johnsen score, Plzf, Gfrα-1, Scp-1, Tekt-1 expressions, and histopathological profile) were significantly decreased following testicular torsion (group 3) when compared to the control group (p ≤ 0.05). However, all abovementioned parameters showed a significant increase/improvement in torsion-transplantation group compared to torsion group. However, these parameters in Capsule SSCs transplantation increase relative expression of pre-/postmeiotic genes and improve sperm parameters and testis structure in testicular torsion-detorsion mice.Summary sentence SSCs transplantation increase relative expression of pre-/post-meiotic genes and improve sperm parameters and testis structure in the ischemic condition. the TT group were significantly lower in the sham and control groups (p ≤ 0.05).Conclusion SSCs transplantation could up-regulate the expression of pre-and post-meiotic genes in testicular ischemia, which resulted in improvement of both testicular function and structure after testicular torsion.

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Cyclical and Patch-Like GDNF Distribution along the Basal Surface of Sertoli Cells in Mouse and Hamster Testes

Cited by 49 publications

References 52 publications

A novel Amh-Treck transgenic mouse line allows toxin-dependent loss of supporting cells in gonads

A novel Amh-Treck transgenic mouse line allows toxin-dependent loss of supporting cells in gonads

The effects of poly L-lactic acid nanofiber scaffold on mouse spermatogonial stem cell culture

Alteration of spermatogenesis following spermatogonial stem cells transplantation in testicular torsion-detorsion mice

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