Cyclic voltammetry: A new strategy for the evaluation of oxidative damage to bovine insulin

Abstract

Research on protein oxidative damage may give insight into the nature of protein functions and pathological conditions. In this work, the oxidative damage of bovine insulin on Au electrode was investigated by cyclic voltammetry (CV). The experimental results show that there are two anodic peaks for the oxidative damage of bovine insulin, which arise from the oxidation of the exposed disulfide bond SAS CYS7A,CYS7B , forming sulfenic acid RSOH (1.20 V, vs. SCE), sulfinic acid RSO 2 H and sulfonic acid RSO 3 H (1.35 V, vs. SCE). These in vitro findings not only demonstrate the applicability of CV in simulating/evaluating the oxidative damage of nonredox proteins but also find two promising candidates (two anodic peaks) for measuring insulin.

Carbon nanotubes based supercapacitor for hydrocarbon sensing in liquid state

“…Then the electrodes were rinsed with milli Q water and dipped into piranha solution for 10 min as described by Zong et al [13]. After that the electrodes were rinsed with milli Q water again preceded by ethanol in a bath sonicator (Model no.…”

Release of insulin granules by simultaneous, high‐speed correlative SICM‐FCM

“…Biophysical alternatives to fluorescence microscopy of single‐vesicle exocytosis are nominally free from the label problem; at the same time, they are less informative than imaging and, again, do not measure insulin release directly. Monitoring of single‐vesicle exocytosis using patch‐clamp electrophysiology 10 or electrochemical techniques 11,12 provides excellent signal‐to‐noise ratio but largely lacks spatial information. This problem may be resolved by using scanning probe microscopy (SPM) techniques.…”

Release of insulin granules by simultaneous, high-speed correlative SICM-FCM

“…Biophysical alternatives to fluorescence microscopy of single-vesicle exocytosis are nominally free from the label problem; at the same time, they are less informative than imaging and, again, do not measure insulin release directly. Monitoring of single-vesicle exocytosis using patch-clamp electrophysiology (Neher & Marty, 1982) or electrochemical techniques (Huang et al, 1995;Zong et al, 2010) provides excellent signal-to-noise ratio but largely lacks spatial information. This problem may be resolved by using scanning probe microscopy (SPM) techniques.…”