Cyclic‐di‐GMP signalling meets extracellular polysaccharide synthesis in <i>Bacillus subtilis</i>

Kampf, Jan; Stülke, Jörg

doi:10.1111/1758-2229.12530

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…C-di-GMP has been identified as an important signaling molecule that controls and regulates various cellular functions, such as virulence, motility (motile to sessile), biofilm formation and dispersion, flagellar motility, adhesion to surfaces, cell-cell signaling and cell differentiation [77]. C-di-GMP is involved in bacterial exopolysaccharide synthesis and biofilm formation in many bacterial species, such as B. subtilis and P. aeruginosa [78,79]. C-di-GMP performs its regulatory function by binding to a diverse group of effectors, such as kinases or phosphorylases [80], transcription factors [81], PilZ domain proteins [82], and riboswitches [83].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Computational Prediction and Analysis of Noncoding RNAs in Oleidesulfovibrio alaskensis G20

Singh,

Sani

2024

Microorganisms

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Noncoding RNAs (ncRNAs) play key roles in the regulation of important pathways, including cellular growth, stress management, signaling, and biofilm formation. Sulfate-reducing bacteria (SRB) contribute to huge economic losses causing microbial-induced corrosion through biofilms on metal surfaces. To effectively combat the challenges posed by SRB, it is essential to understand their molecular mechanisms of biofilm formation. This study aimed to identify ncRNAs in the genome of a model SRB, Oleidesulfovibrio alaskensis G20 (OA G20). Three in silico approaches revealed genome-wide distribution of 37 ncRNAs excluding tRNAs in the OA G20. These ncRNAs belonged to 18 different Rfam families. This study identified riboswitches, sRNAs, RNP, and SRP. The analysis revealed that these ncRNAs could play key roles in the regulation of several pathways of biosynthesis and transport involved in biofilm formation by OA G20. Three sRNAs, Pseudomonas P10, Hammerhead type II, and sX4, which were found in OA G20, are rare and their roles have not been determined in SRB. These results suggest that applying various computational methods could enrich the results and lead to the discovery of additional novel ncRNAs, which could lead to understanding the “rules of life of OA G20” during biofilm formation.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Computational Prediction and Analysis of Noncoding RNAs in Oleidesulfovibrio alaskensis G20

Singh,

Sani

2024

Microorganisms

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“…The EPS produced by B. subtilis is mainly attributed to epsA-O operon and sacB-yveB-yveA transcriptional unit (Branda et al, 2006;Vlamakis et al, 2013;Arnaouteli et al, 2021). Recently, an additional unknown EPS encoded by ydaJKLMN operon was reported to contribute to extracellular matrix and thus biofilm formation in B. subtilis under laboratory conditions (Kampf and Stulke, 2017;Bedrunka and Graumann, 2017b). The analysis of the contributions of the different genes revealed that the YdaL, YdaM, and YdaN proteins provide the catalytic activities for EPS synthesis and YdaJ modifies the polysaccharide.…”

Section: Introductionmentioning

confidence: 99%

“…The analysis of the contributions of the different genes revealed that the YdaL, YdaM, and YdaN proteins provide the catalytic activities for EPS synthesis and YdaJ modifies the polysaccharide. YdaK, which is also encoded within the putative EPS-synthesis operon ydaJ-N, is a cyclic di-GMP (c-di-GMP) binding signal transduction protein and is indispensable for EPS biosynthesis and complex colony biofilm formation in B. subtilis (Kampf and Stulke, 2017;Bedrunka and Graumann, 2017b). However, the function of the unknown EPS produced by YdaLMN is unclear.…”

Section: Introductionmentioning

confidence: 99%

Cyclic di-GMP regulates bacterial colonization and further biocontrol efficacy of Bacillus velezensis against apple ring rot disease via its potential receptor YdaK

et al. 2022

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Bacillus species are among the most investigated beneficial bacteria and widely used in agricultural systems as biological control agents. Its biocontrol efficacy is controlled by diverse regulators. Cyclic diguanylate (c-di-GMP) is a nearly universal second messenger in bacteria and modulates various important physiological processes, including motility, biofilm formation, antifungal antibiotic production and host colonization. However, the impact of c-di-GMP on biocontrol efficacy of beneficial bacteria is unknown. Bacillus velezensis PG12 is an effective biocontrol strain against apple ring rot disease caused by Botryosphaeria dothidea. In this study, the contribution of c-di-GMP to biocontrol efficacy of B. velezensis PG12 was investigated. Deletion of single gene encoding diguanylate cyclase or phosphodiesterase did not affect its biocontrol efficacy against apple ring rot. However, artificial modulation of c-di-GMP level in the cells leads to a significant change of biocontrol efficacy, suggesting that c-di-GMP positively regulates biocontrol efficacy of B. velezensis PG12 against apple ring rot disease. More evidences indicate that c-di-GMP does not affect the antagonistic activity of B. velezensis PG12 against B. dothidea in vitro and in vivo, but positively regulates biofilm formation of B. velezensis PG12 and its colonization on apple fruits. Importantly, deletion of ydaK could rescue the inhibition of biofilm formation, bacterial colonization and biocontrol efficacy caused by low c-di-GMP level, indicating that YdaK is the potential c-di-GMP receptor to regulate biofilm formation, colonization and effective biological control. However, YdaK did not affect the antagonistic activity of B. velezensis PG12 against B. dothidea. Based on these findings, we propose that c-di-GMP regulates biofilm formation, subsequently the bacterial colonization on apple fruits and thus biocontrol efficacy of B. velezensis through its receptor YdaK. This is the first report showing that c-di-GMP plays a role in biocontrol efficacy of beneficial bacteria.

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Role of c-di-GMP in improving stress resistance of alginate-chitosan microencapsulated Bacillus subtilis cells in simulated digestive fluids

et al. 2021

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Cyclic‐di‐GMP signalling meets extracellular polysaccharide synthesis in Bacillus subtilis

Cited by 5 publications

References 15 publications

Genome-Wide Computational Prediction and Analysis of Noncoding RNAs in Oleidesulfovibrio alaskensis G20

Genome-Wide Computational Prediction and Analysis of Noncoding RNAs in Oleidesulfovibrio alaskensis G20

Cyclic di-GMP regulates bacterial colonization and further biocontrol efficacy of Bacillus velezensis against apple ring rot disease via its potential receptor YdaK

Role of c-di-GMP in improving stress resistance of alginate-chitosan microencapsulated Bacillus subtilis cells in simulated digestive fluids

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