Cyclic di-GMP Signaling Links Biofilm Formation and Mn(II) Oxidation in Pseudomonas resinovorans

Abstract

The presence of Mn(II) in groundwater, a common source of drinking water, is a cause of water quality impairment, interfering with its disinfection, causing operation problems, and affecting human health. Purification of groundwater containing Mn(II) plays an important role in environmental and social safety.

Evolution of cyclic di-GMP signalling on a short and long term time scale

“…In concurrence with its ancient phylogenetic origin and abundance [17], cyclic di-GMP signalling affects molecular mechanisms from modulating the binding properties of transcription factors to regulation of enzymatic activities and protein-protein interactions. Thereby, fundamental microbial physiological and metabolic processes can be affected which span from the oxidation of Mn 2+ , a process that is required for water clearance, the differential use of carbon sources and differential biofilm formation in host cells to the role of cyclic di-GMP as an extracellular signalling molecule [18][19][20][21]. Due to the sheer number of domains throughout the phylogenetic tree, the system is subject to substantial evolution.…”

Sublethal nickel toxicity shuts off manganese oxidation and pellicle biofilm formation in Pseudomonas putidaGB‐1

“…Yet, the impairment of Mn oxide production in Ni‐stressed P. putida GB‐1 was not substrate dependent (i.e., cultures in both liquid and solid media experienced a complete halt in Mn oxidation), which more strongly supports the notion that Mn oxidation is turned off when Ni detoxification intensifies despite the status of flagella development. Biofilm formation and Mn oxidation were also tightly associated in P. resinovorans MOB‐513, but the relationship was explained by the intracellular levels of the c‐di‐GMP second messenger (Piazza et al, 2022). Strains with decreased levels of c‐di‐GMP characterized by low biofilm showed delayed and lower Mn oxidation activity.…”

“…The overexpression of bifA leads to lower intracellular concentration of the c-di-GMP second messenger, which induces flagellar-mediated motility and thus decreases biofilm incidence (Jiménez-Fern andez et al, 2014). A strong relationship between intracellular levels of c-di-GMP, biofilm formation and Mn oxidation was recently observed in a Mn-oxidizing environmental isolate of Pseudomonas resinovorans (Piazza et al, 2022). The overexpression of a phosphodiesterase gene in P. resinovorans MOB-513 resulted in low intracellular c-di-GMP and, similar to our results, led to reduced biofilm incidence and increased cell motility.…”

BatR: A novel regulator of antibiotic tolerance inPseudomonas aeruginosabiofilms

“…The upregulation of genes linked to stationary phase adaptation, environmental stress and anaerobiosis further underscores the distinct features associated with biofilm growth (11, 12). Additionally, the spatial arrangement of cells within the biofilm community, exposed to multiple resource gradients, introduces heterogeneity in cell physiology and metabolism that plays an important role in antibiotic tolerance.…”

“…The upregulation of genes linked to stationary phase adaptation, environmental stress and anaerobiosis further underscores the distinct features associated with biofilm growth (11,12).…”