Cyclic di-GMP Modulates a Metabolic Flux for Carbon Utilization in Salmonella enterica Serovar Typhimurium

Baek, Jin Wook; Yoon, Hyunjin

doi:10.1128/spectrum.03685-22

Cited by 4 publications

(2 citation statements)

References 85 publications

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“…The other screened vaccine candidate, BcsZ, is a type of cellulase that, is secreted and located outside the cell membrane. It plays an important role in various cellular processes such as cell clumping, biofilm formation, flagella-dependent motility, and efficient pathogen-host interactions [30,31]. We hypothesize that antiserum or antibodies generated against BcsZ could potentially block the active site and inhibit Salmonella colonization.…”

Section: Discussionmentioning

confidence: 99%

Salmonella typhimurium targeting with monoclonal antibodies prevents infection in mice

Li,

Yang,

Fan

et al. 2023

PLoS Negl Trop Dis

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Salmonella is a prevalent foodborne and waterborne pathogens threating global public health and food safety. Given the diversity of Salmonella serotypes and the emergence of antibiotic-resistant strains, there is an urgent need for the development of broadly protective therapies. This study aims to prepare monoclonal antibodies (Mabs) with broad reactivity against multi-serotype Salmonella strains, potentially offering cross-protection. We prepared two Mabs F1D4 and B7D4 against protein FliK and BcsZ, two potential vaccine candidates against multi-serotype Salmonella. The two Mabs belonging to IgG1 isotype exhibited high titers of 1:256,000 and 1:512,000 respectively, as well as broad cross-reactivity against 28 different serotypes of Salmonella strains with percentages of 89.29% and 92.86%, correspondingly. Neutralizing effects of the two Mabs on Salmonella growth, adhesion, invasion and motility was evaluated in vitro using bacteriostatic and bactericidal activity with and without complement and bacterial invasion inhibition assay. Additionally, cytotoxicity assays, animal toxicity analyses, and pharmacokinetic evaluations demonstrated the safety and sustained effectiveness of both Mabs. Furthermore, F1D4 or B7D4-therapy in mice challenged with S. Typhimurium LT2 exhibited milder organs damage and lower Salmonella colonization, as well as the higher relative survival of 86.67% and 93.33% respectively. This study produced two broadly reactive and potential cross protective Mabs F1D4 and B7D4, which offered new possibilities for immunotherapy of salmonellosis.

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Section: Discussionmentioning

confidence: 99%

Salmonella typhimurium targeting with monoclonal antibodies prevents infection in mice

Li,

Yang,

Fan

et al. 2023

PLoS Negl Trop Dis

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“…This gene regulates the expression of the csgD gene, which is involved in the formation of curli and cellulose, important components of biofilms [86]. Furthermore, the Scoary analysis showed a significant association between S. Give and the presence of a multiphosphoryl transfer protein, the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) fructose transporter subunit IIB gene, and PTS fructose transporter subunit IIC genes, which facilitate the uptake of extracellular sugars [87]. In S. Give, the sugars transported by PTS might assist in biofilm formation, as reported in Vibrio cholerae [88].…”

Section: Discussionmentioning

confidence: 99%

Genomic Diversity, Antimicrobial Resistance, Plasmidome, and Virulence Profiles of Salmonella Isolated from Small Specialty Crop Farms Revealed by Whole-Genome Sequencing

Bhandari,

Poelstra,

Kauffman

et al. 2023

Antibiotics

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Salmonella is the leading cause of death associated with foodborne illnesses in the USA. Difficulty in treating human salmonellosis is attributed to the development of antimicrobial resistance and the pathogenicity of Salmonella strains. Therefore, it is important to study the genetic landscape of Salmonella, such as the diversity, plasmids, and presence antimicrobial resistance genes (AMRs) and virulence genes. To this end, we isolated Salmonella from environmental samples from small specialty crop farms (SSCFs) in Northeast Ohio from 2016 to 2021; 80 Salmonella isolates from 29 Salmonella-positive samples were subjected to whole-genome sequencing (WGS). In silico serotyping revealed the presence of 15 serotypes. AMR genes were detected in 15% of the samples, with 75% exhibiting phenotypic and genotypic multidrug resistance (MDR). Plasmid analysis demonstrated the presence of nine different types of plasmids, and 75% of AMR genes were located on plasmids. Interestingly, five Salmonella Newport isolates and one Salmonella Dublin isolate carried the ACSSuT gene cassette on a plasmid, which confers resistance to ampicillin, chloramphenicol, streptomycin, sulfonamide, and tetracycline. Overall, our results show that SSCFs are a potential reservoir of Salmonella with MDR genes. Thus, regular monitoring is needed to prevent the transmission of MDR Salmonella from SSCFs to humans.

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Advances in transcriptomic analysis of Salmonella biofilms and their correlation with food safety

Meng,

Lyu,

Bie

et al. 2024

Current Opinion in Food Science

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Cyclic di-GMP Modulates a Metabolic Flux for Carbon Utilization in Salmonella enterica Serovar Typhimurium

Abstract: Cyclic di-GMP is a central signaling molecule that determines the transition between motile and nonmotile lifestyles in many bacteria. It stimulates biofilm formation at high concentrations but leads to biofilm dispersal and planktonic status at low concentrations.

Cited by 4 publications

References 85 publications

Salmonella typhimurium targeting with monoclonal antibodies prevents infection in mice

Salmonella typhimurium targeting with monoclonal antibodies prevents infection in mice

Genomic Diversity, Antimicrobial Resistance, Plasmidome, and Virulence Profiles of Salmonella Isolated from Small Specialty Crop Farms Revealed by Whole-Genome Sequencing

Advances in transcriptomic analysis of Salmonella biofilms and their correlation with food safety

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