Cyclic Appearance of Defective Interfering Particles of Herpes Simplex Virus and the Concomitant Accumulation of Early Polypeptide VP175

Murray, Byron K.; Biswal, Nrusingh C.; Bookout, Jack B.; Lanford, Robert E.; Courtney, Richard J.; Melnick, Joseph L.

doi:10.1159/000149894

Cited by 50 publications

(24 citation statements)

References 7 publications

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“…The individual yields of infectious virus are summarized in figure I, together with the applied MOI, and demonstrate that minima of infectious virus yields were observed periodically at every third passage, as referred to the first minimum. Similar results were obtained by M urray et al [4] for other HSV-I and -2 strains.…”

Section: Periodic Reduction O F Infectious Virus Yields During High Msupporting

confidence: 90%

Synthesis and Packaging of Herpes Simplex Virus DNA in the Course of Virus Passages at High Multiplicity

Stegmann¹,

Zentgraf²,

Ott³

et al. 1978

Intervirology

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Herpes simplex virus type 1 (HSV-1), strain ANG, was passaged serially at high MOI or undiluted on RC-37 cells. The yields of infectious virus decreased and increased periodically with a maximum of about 3 log units variation in titer. The amount of newly synthesized HSV-1 DNA, however, did not vary by more than a factor of 2. On the other hand, the fraction of total HSV-1 DNA associated with virions was reduced at passage numbers coinciding with the minima in the yields of infectious progeny virus. Apparently, low yields of infectious virus mainly reflected a reduced efficiency of packaging of viral DNA into mature virus particles. One step in the process of virus maturation which appeared to be impaired was the assembly of nucleocapsids. HSV-1 ANG was shown to be capable of generating different classes of defective particles in independent series of virus passages at high MOI.

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Section: Periodic Reduction O F Infectious Virus Yields During High Msupporting

confidence: 90%

Synthesis and Packaging of Herpes Simplex Virus DNA in the Course of Virus Passages at High Multiplicity

Stegmann¹,

Zentgraf²,

Ott³

et al. 1978

Intervirology

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“…The cyclic appearance and disappearance of defective particles (Murray et al, 1975), if it occurred in VZV, might within a few passages, result in the appearance of DNA molecules with altered restriction patterns. Therefore, the DNA of VZV-KMcC at passage levels 40, 42, 44 and 46 (in WI-38 ceils) was analysed.…”

Section: Restriction Endonuclease Analysis Of the Dna From Variceua-zmentioning

confidence: 99%

Restriction Endonuclease Analysis of the DNA from Varicella-Zoster Virus: Stability of the DNA after Passage in vitro

Zweerink¹,

Morton

Stanton

et al. 1981

Journal of General Virology

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“…When HSV-1 is serially passaged at a high multiplicity of infection, virus stocks are produced which interfere with the infectivity of standard (non-defective) virus (Bronson et al, 1973;Murray et al, 1975 ;0000-6320 © 1985 (1984) suggested that these two signals may have to be on a contiguous DNA fragment for interference to occur, but the interpretation of their experiments is complicated by the fact that the defective genomes present within a virus stock generally represent a heterogeneous collection of molecules of incompletely defined sequence. In the present studies we have therefore attempted to extend these observations by using plasmids containing cloned copies of functional or inactivated ORIs and the viral 'a' sequence either alone or in combination.…”

Section: Introductionmentioning

confidence: 99%

Mutagenesis of a Herpes Simplex Virus Origin of DNA Replication and its Effect on Viral Interference

Stow

1985

Journal of General Virology

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SUMMARYSupercoiled plasmid molecules containing cloned copies of a DNA fragment which includes a functional herpes simplex virus type 1 origin of DNA replication were cleaved preferentially at two positions within the viral insert by nuclease S1. Plasmids with molecular linker insertions at these sites were constructed, and analysis of two representative plasmids demonstrated the presence of palindromic DNA sequences at the preferred cleavage positions. One of these palindromic sequences occurred within a 90 bp region in which the cis-acting sequences essential for viral origin function had previously been located, Insertion of a linker at this position abolished origin activity, demonstrating an essential role for sequences within the palindrome in the initiation of DNA synthesis. In transfection assays, plasmids containing a functional viral origin of DNA replication markedly interfered with the infectivity of non-defective viral DNA even in the absence of viral encapsidation signals. Inactivation of the origin greatly reduced this effect on DNA infectivity, suggesting that viral interference may be mediated by a mechanism involving the replicative machinery.

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Cyclic Appearance of Defective Interfering Particles of Herpes Simplex Virus and the Concomitant Accumulation of Early Polypeptide VP175

Cited by 50 publications

References 7 publications

Synthesis and Packaging of Herpes Simplex Virus DNA in the Course of Virus Passages at High Multiplicity

Synthesis and Packaging of Herpes Simplex Virus DNA in the Course of Virus Passages at High Multiplicity

Restriction Endonuclease Analysis of the DNA from Varicella-Zoster Virus: Stability of the DNA after Passage in vitro

Mutagenesis of a Herpes Simplex Virus Origin of DNA Replication and its Effect on Viral Interference

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