CyanoFactory, a European consortium to develop technologies needed to advance cyanobacteria as chassis for production of chemicals and fuels

Lindblad, Peter; Fuente, David; Borbe, Friederike; Cicchi, Bernardo; Conejero, J. Alberto; Couto, Narciso; Čelešnik, Helena; Diano, Marcello; Dolinar, Marko; Esposito, Serena; Evans, Caroline; Ferreira, Eunice A.; Keller, Joseph; Khanna, Namita; Kind, Gabriel M.; Landels, Andrew; Lemus, Lenin; Noirel, Josselin; Ocklenburg, Sarah; Oliveira, Paulo; Pacheco, Catarina C.; Parker, Jennifer; Pereira, José A.; Pham, Trong Khoa; Pinto, Filipe; Rexroth, Sascha; Rögner, Matthias; Schmitz, H.-U.; Benavides, Ana Margarita Silva; Siurana, María Pilar Santamarina; Tamagnini, Paula; Touloupakis, Eleftherios; Torzillo, Giuseppe; Urchueguı́a, Javier F.; Wegelius, Adam; Wiegand, Katrin; Wright, Phillip C.; Wutschel, Mathias; Wünschiers, Röbbe

doi:10.1016/j.algal.2019.101510

Cited by 27 publications

(13 citation statements)

References 53 publications

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“…29.9 mg DW L -1 d -1 in white light and 2xBG11, Fig. 1 C and Table 1 ) was at a lower end of that typically reported for A. platensis (23–750 mg DW L -1 d -1 ) ( Schipper et al, 2020 ), Lindblad et al (2019) have shown that Synechocystis can achieve a productivity of 185 mg DW L -1 d -1 in a scalable (1350 L) flat-panel PBR design under daylight conditions. Based on the Te-PC content in this study, the estimated Te-PC productivity of the TeBACD strain in an optimised flat-panel PBR (i.e.…”

Section: Resultsmentioning

confidence: 62%

“…Recently, peptides from plants, animals and prokaryotes have been expressed at 10–20% of total cellular protein, thus setting the foundation for Synechocystis as recombinant protein production platform ( Zhang et al, 2021 ). However, to date scale-up production (10–1300 L) has only been demonstrated for the production of polyhydroxybutyrate (PHB) and hydrogen ( Lindblad et al, 2019 ). To further promote Synechocystis as robust and scalable biofactory, cell harvesting and disruption methods must be demonstrated at larger scales.…”

Section: Introductionmentioning

confidence: 99%

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Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803

Puzorjov

Unal

Wear

et al. 2022

Bioresource Technology

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Section: Resultsmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803

Puzorjov

Unal

Wear

et al. 2022

Bioresource Technology

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“…Large scale research initiatives are starting to address cyanobacteria as green cell factories. This can be exemplified by the so-called "CyanoFactory" approach, which includes, for example, the development of needed genetic tools, improved photosynthetic efficiencies, analyses and understanding of the modified metabolism with its further modeling, design and development of efficient photobioreactor systems, and biosafety aspects [54]. These photosynthetic micro-factories are increasingly considered important biocatalysts for the production of renewable and carbon-neutral chemicals and fuels from carbon dioxide and solar energy.…”

Section: Past and Present Applications Involving Microalgae And Cyano...mentioning

confidence: 99%

Hints at the Applicability of Microalgae and Cyanobacteria for the Biodegradation of Plastics

et al. 2020

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Massive plastic accumulation has been taking place across diverse landscapes since the 1950s, when large-scale plastic production started. Nowadays, societies struggle with continuously increasing concerns about the subsequent pollution and environmental stresses that have accompanied this plastic revolution. Degradation of used plastics is highly time-consuming and causes volumetric aggregation, mainly due to their high strength and bulky structure. The size of these agglomerations in marine and freshwater basins increases daily. Exposure to weather conditions and environmental microflora (e.g., bacteria and microalgae) can slowly corrode the plastic structure. As has been well documented in recent years, plastic fragments are widespread in marine basins and partially in main global rivers. These are potential sources of negative effects on global food chains. Cyanobacteria (e.g., Synechocystis sp. PCC 6803, and Synechococcus elongatus PCC 7942), which are photosynthetic microorganisms and were previously identified as blue-green algae, are currently under close attention for their abilities to capture solar energy and the greenhouse gas carbon dioxide for the production of high-value products. In the last few decades, these microorganisms have been exploited for different purposes (e.g., biofuels, antioxidants, fertilizers, and ‘superfood’ production). Microalgae (e.g., Chlamydomonas reinhardtii, and Phaeodactylum tricornutum) are also suitable for environmental and biotechnological applications based on the exploitation of solar light. Can photosynthetic bacteria and unicellular eukaryotic algae play a role for further scientific research in the bioremediation of plastics of different sizes present in water surfaces? In recent years, several studies have been targeting the utilization of microorganisms for plastic bioremediation. Among the different phyla, the employment of wild-type or engineered cyanobacteria may represent an interesting, environmentally friendly, and sustainable option.

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“…Starting as poorly adapted microorganisms for genetic engineering, advances in the last decade have made them attractive organisms for biosynthetic production of commodities ranging from biofuels to high added-value compounds. A book ( Zhang & Song, 2018 ), several book chapters ( e.g ., Klemenčič et al, 2017 ; Badary & Sode, 2020 ; Hudson, 2021 ) and review articles ( Khan et al, 2019 ; Lindblad et al, 2019 ; Santos-Merino, Singh & Ducat, 2019 ; Vavitsas et al, 2019 ; Ng, Keskin & Tan, 2020 ; Wang, Gao & Yang, 2020 ; Liu et al, 2021 ; Sproles et al, 2021 ) published in recent years emphasize the potential of cyanobacteria and present encouraging results obtained with these hosts.…”

Section: Introductionmentioning

confidence: 99%

A chimeric vector for dual use in cyanobacteria and Escherichia coli, tested with cystatin, a nonfluorescent reporter protein

Juteršek

Dolinar

2021

PeerJ

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Background Developing sustainable autotrophic cell factories depends heavily on the availability of robust and well-characterized biological parts. For cyanobacteria, these still lag behind the more advanced E. coli toolkit. In the course of previous protein expression experiments with cyanobacteria, we encountered inconveniences in working with currently available RSF1010-based shuttle plasmids, particularly due to their low biosafety and low yields of recombinant proteins. We also recognized some drawbacks of the commonly used fluorescent reporters, as quantification can be affected by the intrinsic fluorescence of cyanobacteria. To overcome these drawbacks, we envisioned a new chimeric vector and an alternative reporter that could be used in cyanobacterial synthetic biology and tested them in the model cyanobacterium Synechocystis sp. PCC 6803. Methods We designed the pMJc01 shuttle plasmid based on the broad host range RSFmob-I replicon. Standard cloning techniques were used for vector construction following the RFC10 synthetic biology standard. The behavior of pMJC01 was tested with selected regulatory elements in E. coli and Synechocystis sp. PCC 6803 for the biosynthesis of the established GFP reporter and of a new reporter protein, cystatin. Cystatin activity was assayed using papain as a cognate target. Results With the new vector we observed a significantly higher GFP expression in E. coli and Synechocystis sp. PCC 6803 compared to the commonly used RSF1010-based pPMQAK1. Cystatin, a cysteine protease inhibitor, was successfully expressed with the new vector in both E. coli and Synechocystis sp. PCC 6803. Its expression levels allowed quantification comparable to the standardly used fluorescent reporter GFPmut3b. An important advantage of the new vector is its improved biosafety due to the absence of plasmid regions encoding conjugative transfer components. The broadhost range vector pMJc01 could find application in synthetic biology and biotechnology of cyanobacteria due to its relatively small size, stability and ease of use. In addition, cystatin could be a useful reporter in all cell systems that do not contain papain-type proteases and inhibitors, such as cyanobacteria, and provides an alternative to fluorescent reporters or complements them.

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CyanoFactory, a European consortium to develop technologies needed to advance cyanobacteria as chassis for production of chemicals and fuels

Cited by 27 publications

References 53 publications

Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803

Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803

Hints at the Applicability of Microalgae and Cyanobacteria for the Biodegradation of Plastics

A chimeric vector for dual use in cyanobacteria and Escherichia coli, tested with cystatin, a nonfluorescent reporter protein

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