Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803

Puzorjov, Anton; Unal, Suleyman Mert; Wear, Martin A.; McCormick, Alistair J.

doi:10.1016/j.biortech.2021.126459

Cited by 9 publications

(1 citation statement)

References 44 publications

(62 reference statements)

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“…Cyanobacteria, represented by Spirulina, are excellent hosts for the production of phycocyanin [39,40]. However, the Spirulina gene transformation system is not yet mature enough to make Spirulina an engineered host to further enhance the production of phycocyanin.…”

Section: Enhanced Production Of Phycocyanin In Mutant Strains Of Syne...mentioning

confidence: 99%

Promoting Heme and Phycocyanin Biosynthesis in Synechocystis sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering

Cao,

Wang,

Sun

et al. 2023

Marine Drugs

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Due to their unique biochemical and spectroscopic properties, both heme and phycocyanobilin are widely applied in the medical and food industries. Synechocystis sp. PCC 6803 contains both heme and phycocyanin, and is capable of synthesizing phycocyanin using heme as a precursor. The aim of this study was to uncover viable metabolic targets in the porphyrin pathway from Synechocystis sp. PCC 6803 to promote the accumulation of heme and phycocyanin in the recombinant strains of microalgae. A total of 10 genes related to heme synthesis pathway derived from Synechococcus elongatus PCC 7942 and 12 genes related to endogenous heme synthesis were individually overexpressed in strain PCC 6803. The growth rate and pigment content (heme, phycocyanin, chlorophyll a and carotenoids) of 22 recombinant algal strains were characterized. Quantitative real-time PCR technology was used to investigate the molecular mechanisms underlying the changes in physiological indicators in the recombinant algal strains. Among the 22 mutant strains, the mutant overexpressing the haemoglobin gene (glbN) of strain PCC 6803 had the highest heme content, which was 2.5 times higher than the wild type; the mutant overexpressing the gene of strain PCC 7942 (hemF) had the highest phycocyanin content, which was 4.57 times higher than the wild type. Overall, the results suggest that genes in the porphyrin pathway could significantly affect the heme and phycocyanin content in strain PCC 6803. Our study provides novel crucial targets for promoting the accumulation of heme and phycocyanin in cyanobacteria.

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Section: Enhanced Production Of Phycocyanin In Mutant Strains Of Syne...mentioning

confidence: 99%

Promoting Heme and Phycocyanin Biosynthesis in Synechocystis sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering

Cao,

Wang,

Sun

et al. 2023

Marine Drugs

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Enhanced growth, chlorophyll a and phycobiliprotein content, and modulation of bioactive metabolite profiles in Synechocystis sp. PCC 6803 culture by ( +)-usnic acid

et al. 2023

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Valuable pigments from microalgae: phycobiliproteins, primary carotenoids, and fucoxanthin

Zittelli¹,

Lauceri

Faraloni³

et al. 2023

Photochem Photobiol Sci

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Phycobiliproteins, carotenoids and fucoxanthin are photosynthetic pigments extracted from microalgae and cyanobacteria with great potential biotechnological applications, as healthy food colorants and cosmetics. Phycocyanin possesses a brilliant blue color, with fluorescent properties making it useful as a reagent for immunological essays. The most important source of phycocyanin is the cyanobacterium Arthrospiraplatensis, however, recently, the Rhodophyta Galdieriasulphuraria has also been identified as such. The main obstacle to the commercialization of phycocyanin is represented by its chemical instability, strongly reducing its shelf-life. Moreover, the high level of purity needed for pharmaceutical applications requires several steps which increase both the production time and cost. Microalgae (Chlorella, Dunaliella, Nannochloropsis, Scenedesmus) produce several light harvesting carotenoids, and are able to manage with oxidative stress, due to their free radical scavenging properties, which makes them suitable for use as source of natural antioxidants. Many studies focused on the selection of the most promising strains producing valuable carotenoids and on their extraction and purification. Among carotenoids produced by marine microalgae, fucoxanthin is the most abundant, representing more than 10% of total carotenoids. Despite the abundance and diversity of fucoxanthin producing microalgae only a few species have been studied for commercial production, the most relevant being Phaeodactylumtricornutum. Due to its antioxidant activity, fucoxanthin can bring various potential benefits to the prevention and treatment of lifestyle-related diseases. In this review, we update the main results achieved in the production, extraction, purification, and commercialization of these important pigments, motivating the cultivation of microalgae as a source of natural pigments. Graphical abstract

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Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803

Abstract: Graphical abstract

Cited by 9 publications

References 44 publications

Promoting Heme and Phycocyanin Biosynthesis in Synechocystis sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering

Promoting Heme and Phycocyanin Biosynthesis in Synechocystis sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering

Enhanced growth, chlorophyll a and phycobiliprotein content, and modulation of bioactive metabolite profiles in Synechocystis sp. PCC 6803 culture by ( +)-usnic acid

Valuable pigments from microalgae: phycobiliproteins, primary carotenoids, and fucoxanthin

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