Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803

Puzorjov, Anton; Unal, Suleyman Mert; Wear, Martin A.; McCormick, Alistair J.

doi:10.1016/j.biortech.2021.126459

Cited by 11 publications

(2 citation statements)

References 44 publications

(62 reference statements)

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“…Cyanobacteria, represented by Spirulina, are excellent hosts for the production of phycocyanin [39,40]. However, the Spirulina gene transformation system is not yet mature enough to make Spirulina an engineered host to further enhance the production of phycocyanin.…”

Section: Enhanced Production Of Phycocyanin In Mutant Strains Of Syne...mentioning

confidence: 99%

Promoting Heme and Phycocyanin Biosynthesis in Synechocystis sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering

Cao,

Wang,

Sun

et al. 2023

Marine Drugs

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Due to their unique biochemical and spectroscopic properties, both heme and phycocyanobilin are widely applied in the medical and food industries. Synechocystis sp. PCC 6803 contains both heme and phycocyanin, and is capable of synthesizing phycocyanin using heme as a precursor. The aim of this study was to uncover viable metabolic targets in the porphyrin pathway from Synechocystis sp. PCC 6803 to promote the accumulation of heme and phycocyanin in the recombinant strains of microalgae. A total of 10 genes related to heme synthesis pathway derived from Synechococcus elongatus PCC 7942 and 12 genes related to endogenous heme synthesis were individually overexpressed in strain PCC 6803. The growth rate and pigment content (heme, phycocyanin, chlorophyll a and carotenoids) of 22 recombinant algal strains were characterized. Quantitative real-time PCR technology was used to investigate the molecular mechanisms underlying the changes in physiological indicators in the recombinant algal strains. Among the 22 mutant strains, the mutant overexpressing the haemoglobin gene (glbN) of strain PCC 6803 had the highest heme content, which was 2.5 times higher than the wild type; the mutant overexpressing the gene of strain PCC 7942 (hemF) had the highest phycocyanin content, which was 4.57 times higher than the wild type. Overall, the results suggest that genes in the porphyrin pathway could significantly affect the heme and phycocyanin content in strain PCC 6803. Our study provides novel crucial targets for promoting the accumulation of heme and phycocyanin in cyanobacteria.

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Section: Enhanced Production Of Phycocyanin In Mutant Strains Of Syne...mentioning

confidence: 99%

Promoting Heme and Phycocyanin Biosynthesis in Synechocystis sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering

Cao,

Wang,

Sun

et al. 2023

Marine Drugs

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“…These results are in accordance with previous studies assessing the phycobiliprotein content of Synechocystis strains in synthetic media at lab-scale. For instance, Puzorjov et al (2022) reached a maximum phycocyanin content of 111 mg gDW − 1 with a purity ratio of 0.83 by growing Synechocystis sp. PCC 6803 in BG11; while Touloupakis et al (2015) reached a phycocyanin content of 196 mg gDW − 1 by growing Synechocystis sp.…”

Section: Phycobiliprotein Production Potentialmentioning

confidence: 99%

Phycobiliprotein recovery coupled to the tertiary treatment of wastewater in semi-continuous photobioreactors. Tracking contaminants of emerging concern.

Bellver

Díez-Montero

Escolà

et al. 2023

Bioresource Technology

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Enhanced growth, chlorophyll a and phycobiliprotein content, and modulation of bioactive metabolite profiles in Synechocystis sp. PCC 6803 culture by ( +)-usnic acid

et al. 2023

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Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803

Abstract: Graphical abstract

Cited by 11 publications

References 44 publications

Promoting Heme and Phycocyanin Biosynthesis in Synechocystis sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering

Promoting Heme and Phycocyanin Biosynthesis in Synechocystis sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering

Phycobiliprotein recovery coupled to the tertiary treatment of wastewater in semi-continuous photobioreactors. Tracking contaminants of emerging concern.

Enhanced growth, chlorophyll a and phycobiliprotein content, and modulation of bioactive metabolite profiles in Synechocystis sp. PCC 6803 culture by ( +)-usnic acid

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