Abstract:Phycoerythrins from various cyanobacterial sources have been extensively studied globally for their therapeutic potentials. This study shows the apoptotic potential of cyanobacterial phycoerythrin isolated, purified and characterized from marine Lyngbya sp. A09DM on human lung carcinoma cells (A549 cell). Results indicated that purified cyanobacterial phycoerythrin accounted for a dose-dependent decrement in cell viability, mitochondrial membrane potential and an increment in lactate dehydrogenase release. Hig… Show more
“…Altering the cultivation conditions can make the feed beneficial, since it can be enriched with highly valuable metabolites such as omega fatty acids, carotenoids and essential amino acids [ 11 , 50 , 69 , 70 ]. Consequently, the enriched microalgae can be used as feed supplement to improve the quality of their meat, eggs and milk products which will provide multiple health benefits such as anticancer, antioxidant and antiviral effects to humans when consumed [ 20 , 71 , 72 ] (Table 3 ). Fig.…”
Section: Nutritional Potential Of Microalgae-based Feedmentioning
There is an immediate need to identify alternative sources of high-nutrient feedstocks for domestic livestock production and poultry, not only to support growing food demands but also to produce microalgae-source functional foods with multiple health benefits. Various species of microalgae and cyanobacteria are used to supplement existing feedstocks. In this review, microalgae have been defined as a potential feedstock for domestic animals due to their abundance of proteins, carbohydrates, lipids, minerals, vitamins, and other high-value products. Additionally, the positive physiological effects on products of animals fed with microalgal biomass have been compiled and recommendations are listed to enhance the assimilation of biomolecules in ruminant and nonruminant animals, which possess differing digestive systems. Furthermore, the role of microalgae as prebiotics is also discussed. With regards to large scale cultivation of microalgae for use as feed, many economic trade-offs must be considered such as the selection of strains with desired nutritional properties, cultivation systems, and steps for downstream processing. These factors are highlighted with further investigations needed to reduce the overall costs of cultivation. Finally, this review outlines the pros and cons of utilizing microalgae as a supplementary feedstock for poultry and cattle, existing cultivation strategies, and the economics of large-scale microalgal production.
“…Altering the cultivation conditions can make the feed beneficial, since it can be enriched with highly valuable metabolites such as omega fatty acids, carotenoids and essential amino acids [ 11 , 50 , 69 , 70 ]. Consequently, the enriched microalgae can be used as feed supplement to improve the quality of their meat, eggs and milk products which will provide multiple health benefits such as anticancer, antioxidant and antiviral effects to humans when consumed [ 20 , 71 , 72 ] (Table 3 ). Fig.…”
Section: Nutritional Potential Of Microalgae-based Feedmentioning
There is an immediate need to identify alternative sources of high-nutrient feedstocks for domestic livestock production and poultry, not only to support growing food demands but also to produce microalgae-source functional foods with multiple health benefits. Various species of microalgae and cyanobacteria are used to supplement existing feedstocks. In this review, microalgae have been defined as a potential feedstock for domestic animals due to their abundance of proteins, carbohydrates, lipids, minerals, vitamins, and other high-value products. Additionally, the positive physiological effects on products of animals fed with microalgal biomass have been compiled and recommendations are listed to enhance the assimilation of biomolecules in ruminant and nonruminant animals, which possess differing digestive systems. Furthermore, the role of microalgae as prebiotics is also discussed. With regards to large scale cultivation of microalgae for use as feed, many economic trade-offs must be considered such as the selection of strains with desired nutritional properties, cultivation systems, and steps for downstream processing. These factors are highlighted with further investigations needed to reduce the overall costs of cultivation. Finally, this review outlines the pros and cons of utilizing microalgae as a supplementary feedstock for poultry and cattle, existing cultivation strategies, and the economics of large-scale microalgal production.
“…Likewise, Deniz and Thangam et al demonstrated the prevention activity of phycocyanin against highly lethal lung cancer using a A549 cell model as well [37,38]. In addition, besides phycocyanin, phycoerythrin, which is also derived from cyanobacteria, has been proven to exert pro-apoptotic and anti-proliferative function in A549 cells [39,40], suggesting the potential pharmaceutical value of phycobiliproteins. Overall, the A549 cell line has been widely used in multiple investigations on functional materials; nevertheless, our study employed H358, H1650; and LTEP-a2 NSCLC cell lines to explore the antineoplastic and underlying mechanism of phycocyanin, which has widely illustrated the biological activities of phycocyanin in NSCLC cells.…”
Phycocyanin, derived from Spirulina platensis, is a type of natural antineoplastic marine protein. It is known that phycocyanin exerts anticancer effects on non-small-cell lung cancer (NSCLC) cells, but its underlying mechanism has not been elucidated. Herein, the antitumor function and regulatory mechanism of phycocyanin were investigated in three NSCLC cell lines for the first time: H358, H1650, and LTEP-a2. Cell phenotype experiments suggested that phycocyanin could suppress the survival rate, proliferation, colony formation, and migration abilities, as well as induce apoptosis of NSCLC cells. Subsequently, transcriptome analysis revealed that receptor-interacting serine/threonine-protein kinase 1 (RIPK1) was significantly down-regulated by phycocyanin in the LTEP-a2 cell, which was further validated by qRT-PCR and Western blot analysis in two other cell lines. Interestingly, similar to phycocyanin-treated assays, siRNA knockdown of RIPK1 expression also resulted in growth and migration inhibition of NSCLC cells. Moreover, the activity of NF-κB signaling was also suppressed after silencing RIPK1 expression, indicating that phycocyanin exerted anti-proliferative and anti-migratory function through down-regulating RIPK1/NF-κB activity in NSCLC cells. This study proposes a mechanism of action for phycocyanin involving both NSCLC apoptosis and down regulation of NSCLC genes.
“…It is worth noting that Huang et al investigated the antineoplastic activity of phycocyanin with a novel perspective by using a phycocyanin-based nanocarrier as a nanoplatform, which exerted highly efficient anticancer efficacy [34]. In addition, besides phycocyanin, phycoerythrin, which also derived from cyanobacterial, was proven to exerted anti-proliferative and pro-apoptotic effects on NSCLC A549 cells [35,36], indicating the potential pharmaceutical value of phycobiliproteins. Overall, as an important type of NSCLC cell line, A549 cells were widely used in multiple investigations on functional foods.…”
Phycocyanin is a type of marine functional food additive, exerting a health care efficacy with no side effects. It has been shown that phycocyanin possesses anticancer function in non-small cell lung cancer (NSCLC) cells, but the underlying regulatory mechanism still remains unclear. Further investigation on the antineoplastic mechanism of phycocyanin would provide useful information on NSCLC treatment. In this study, we explored the in vitro function and mechanism of phycocyanin in three typical NSCLC cell lines, H1975, H1650, and LTEP-a2, for the first time. Phenotypic experiments showed that phycocyanin significantly induced the apoptosis as well as suppressed the growth of NSCLC cells. Transcriptome analysis suggested that toll/interleukin 1 receptor domain-containing adaptor protein (TIRAP) was significantly down-regulated by phycocyanin. Strikingly, similar to phycocyanin-treated assays, siRNA knockdown of TIRAP expression also resulted in the anti-proliferative phenomenon in NSCLC cells. In addition, the activity of NF-κB signaling was also suppressed after silencing TIRAP expression, revealing that phycocyanin exerted anti-proliferative function through down-regulating TIRAP/NF-κB activity in NSCLC cells. Collectively, this study has laid a theoretical basis on the treatment of NSCLC and the potential utilization of marine functional products.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
