CXCR4‐SF1 bifunctional adipose‐derived stem cells benefit for the treatment of Leydig cell dysfunction‐related diseases

Abstract

Stem cell transplantation is a candidate method for the treatment of Leydig cell dysfunction‐related diseases. However, there are still many problems that limit its clinical application. Here, we report the establishment of CXCR4‐SF1 bifunctional adipose‐derived stem cells (CXCR4‐SF1‐ADSCs) and their reparative effect on Leydig cell dysfunction. CD29+ CD44+ CD34− CD45− ADSCs were isolated from adipose tissue and purified by fluorescence‐activated cell sorting (FACS). Infection with lentiviruses carrying the CXCR4 and SF1 genes was applied to construct CXCR4‐SF1‐ADSCs. The CXCR4‐SF1‐ADSCs exhibited enhanced migration and had the ability to differentiate into Leydig‐like cells in vitro. Furthermore, the bifunctional ADSCs were injected into BPA‐mediated Leydig cell damage model mice via the tail vein. We found that the CXCR4‐SF1‐ADSCs were capable of homing to the injured testes, differentiating into Leydig‐like cells and repairing the deficiency in reproductive function caused by Leydig cell dysfunction. Moreover, we investigated the mechanism underlying SF1‐mediated differentiation and testosterone synthesis in Leydig cells, and the B‐box and SPRY Domain Containing Protein (BSPRY) gene was proposed to be involved in this process. This study provides insight into the treatment of Leydig cell dysfunction‐related diseases.

The effect of Fumaria parviflora on the expression of sexual hormones along with their receptors in testicles of adult rats induced by varicocele

“…SF‐1 is expressed in pituitary gonadotropes and Leydig cells in men (Li et al, 2020). SF‐1 is primarily responsible for the regulation of multiple components of s teroidogenesis and regulates the function of the reproductive system at all three levels of the HPG (Acevedo‐Rodriguez et al, 2018).…”

Use of polyethyleneoxide and hydroxyethylstarch as blood plasma substitutes in the cryopreservation of testis interstitium cells in mice

“…В деяких дослідженнях показано, що ізольовані клітини інтерстицію (КІ) яєчка, імплантовані до яєчка реципієнта, сприяли відновленню рівня тестостерону [6,7]. Однак через нестачу джерел цих клітин та етичні проблеми популяризація і застосування клітинної трансплантації в клінічній практиці дуже обмежена [8]. Використання кріоконсервованих клітин і створення низькотемпературних запасів клітин для трансплантації може сприяти їх застосуванню в клінічній практиці, а також дає можливість тестувати клітинні препарати на предмет вірусної або бактеріальної контамінації перед використанням.…”