Cuvette-based microfluidic device integrated with nanostructures for measuring dual Localized Surface Plasmon Resonance (LSPR) signals

Abstract

A spectrophotometer that uses a localized surface plasmon resonance phenomenon is a powerful measurement tool in the biotechnology and bioanalysis fields. We propose a novel cuvette design type that can be used for universal spectrophotometers. The novel cuvette design needs a few μl reagent for measuring, and also two chips for measurement can be loaded and measured at the same time. A new cuvette can easily be used several times because of sample chips to be loaded and unloaded since they are mechanically mounted by screws. Therefore, it can offer advantages to users in terms of cost and time. We verify its possibility for use in the biotechnology and bioanalysis fields by a signal enhancement and dual signal detection.

Highly Sensitive and Reliable microRNA Detection with a Recyclable Microfluidic Device and an Easily Assembled SERS Substrate

“…The second step is the modification of SERS substrates with an miR-34a-specific MB (Scheme b). The final stage is the formation of a reusable microfluidic channel mounting the SERS substrate on the cover of the microfluidic device and tightening/untightening the cover using the screws . When the solution is injected into the microfluidic device through the inlet, the void volume in the lower part of the device is filled and the SERS substrate recognizes the target analytes in the solution.…”

“…The final stage is the formation of a reusable microfluidic channel mounting the SERS substrate on the cover of the microfluidic device and tightening/untightening the cover using the screws. 24 When the solution is injected into the microfluidic device through the inlet, the void volume in the lower part of the device is filled and the SERS substrate recognizes the target analytes in the solution. Then, the SERS substrate is unscrewed from the microfluidic device and analyzed via Raman spectroscopy.…”

Highly Dense and Accessible Nanogaps in Au–Ag Alloy Patterned Nanostructures for Surface-Enhanced Raman Spectroscopy Analysis

“…In this study, we propose a successful synthesis of highly dense and accessible nanogaps in Au–Ag alloy nanostructure patterned substrates (HAANs) over a large area (3 × 3 cm 2 , Scheme and Figure S1). Uniform silver dot arrays (SDAs) were synthesized, − and subsequent secondary Au–Ag nanogranule arrays were deposited onto every SDA to produce a lot of uncovered nanogaps. Conventionally, secondary island growth between Au and Ag metal nanostructures is tremendously restricted because of the similar lattice matching between Au and Ag.…”