Cuttlebone used as a bone xenograft in bone healing

Abstract

This study was conducted to examine the potential of cuttlebone xenograft in the healing of bone using radiography and histology for a period of 24 weeks. One hundred and five New Zealand male rabbits with radius defects in the metaphyseal region were divided into five groups treated with cuttlebone, demineralized bone matrix, bovine cancellous graft, and tricalcium phosphate. The control was no treatment. Clinical, radiological, biochemical and histological evaluations were made 1, 2, 3, 4, 6, 12, and 24 weeks after surgery. Physiological measurements (body temperature, heart rate, and respiratory rate) were not affected by the treatments. The radiological score was greatest in the demineralised bone matrix and tricalcium phosphate groups (score of 8), followed by the bovine cancellous graft (score of 6), cuttlebone (score of 6), and control groups (score of 5). The histological score was greatest in the tricalcium phosphate group (score of 55), followed by the cuttlebone (score of 50), bovine cancellous graft (score of 48), demineralized bone matrix (score of 44) and control groups (score of 42). Oxidative enzyme activities were not different across the treatments. The lack of reinfection and infection responses and faster bone union highlight the potential of cuttlebone xenograft in orthopaedic surgery.

Tavşanlarda Mürekkepbalığı Kabuğu ve Kan Pulcuğundan Zengin Plazma ile Birlikte Mürekkep Balığı Kaynaklı Hidroksiapatitin Tibial Kemik Hasarının İyileştirilmesindeki Rolü: Deneysel Bir Çalışma

“…In addition different species of wood were pyrolized and its carbonate residues saturated with calcium salts to achieve bioactive hydroxyapatite [9] . CB is a unique marine biomaterial potentially convertable to hydroxyapatite that has attracted attention of researchers currently [10,13,14,19,20,22] . CB compatibility with bone mineral composition was demonstrated by quantitative analysis of sodium, magnesium, pottasium and calcium ions in CB and human elbow bone [18,19] .…”

“…Cuttlebone (CB) is the hard internal structure of cuttlefish consists of crystals of calcium carbonate (Aragonite) which plays essential role in protecting organism vital organs and act as a floating tank besides. It is biocompatible, osteoconductive and has plasticity in regard to morphology and mineral composition [10,13,[18][19][20] . In addition, this structure is available in many oceans and seas worldwide with low expense.…”

“…Parallel interconnected sheets give CB porosity. The pores diameter is in the range of 200-600 micrometer and therefore they are optimum for new bone formation and neovascularization [19,21] . The special structure and unique characteristics have encouraged researchers to try raw CB or cuttlebone derived hydroxyapatite (CBHA) in the field of tissue engineering and bone healing.…”

Synthetic and Marine-Derived Porous Bone Graft Substitutes

“…6.2.1.1. Natural CB Dogan et al [189] studied the potential of CB as a xenograft by filling a bone defect of a rabbit with CB, demineralized bone matrix (DBM), bovine cancellous graft (BCG), and TCP. At the third postoperative week, it was observed 100% callus formation in the groups treated with CB, DBM and TCP.…”

“…Overall, in the CB group it was observed a high degree of vascularization and good osteogenesis and osteointegration properties. Thus, CB was considered a good xenograft material [189].…”