Cutaneous <scp>pH</scp> landscape as a facilitator of melanoma initiation and progression

Koch, AK; Schwab, Albrecht

doi:10.1111/apha.13105

Cited by 12 publications

(8 citation statements)

References 188 publications

(230 reference statements)

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“…For FTIR and TRIR measurements, DOPA melanin powder was dissolved at 5 mg mL −1 in phosphate-buffered D 2 O solution, containing 25 m m each of sodium phosphate monobasic and sodium phosphate dibasic. The pD value of the buffer and DOPA melanin solution was 7.6 ± 0.1, which is close to what is expected in the stratum spinosum and stratum basale in human skin, where melanocytes produce melanins 51 .…”

Section: Methodssupporting

confidence: 82%

Probing the heterogeneous structure of eumelanin using ultrafast vibrational fingerprinting

et al. 2020

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Eumelanin is a brown-black biological pigment with sunscreen and radical scavenging functions important to numerous organisms. Eumelanin is also a promising redox-active material for energy conversion and storage, but the chemical structures present in this heterogeneous pigment remain unknown, limiting understanding of the properties of its light-responsive subunits. Here, we introduce an ultrafast vibrational fingerprinting approach for probing the structure and interactions of chromophores in heterogeneous materials like eumelanin. Specifically, transient vibrational spectra in the double-bond stretching region are recorded for subsets of electronic chromophores photoselected by an ultrafast excitation pulse tuned through the UV-visible spectrum. All subsets show a common vibrational fingerprint, indicating that the diverse electronic absorbers in eumelanin, regardless of transition energy, contain the same distribution of IR-active functional groups. Aggregation of chromophores diverse in oxidation state is the key structural property underlying the universal, ultrafast deactivation behavior of eumelanin in response to photoexcitation with any wavelength.

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Section: Methodssupporting

confidence: 82%

Probing the heterogeneous structure of eumelanin using ultrafast vibrational fingerprinting

et al. 2020

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“…6 S-nitrosothiols concentration in human skin after irradiation at 320 nm (UVA) in comparison with the control (dark). The skin samples were pre-incubated with a solution of NaNO 3 at different concentrations (50, 500, 1000 and 5000 μmol L −1 ) for 1 h at 25 °C in the dark with the absorption spectra of urocanic acid and melanin, respectively (Gibbsl et al 1993;Geng et al 2008;Zonios et al 2008;Koch and Schwab, 2018;Madkhali et al 2019). Gibbsl et al 1993 showed that urocanic acid has an isomerization reaction at 310-320 nm (Gibbsl et al 1993) and Geng et al 2008 showed that melanin has a protective role against UVC-induced DNA damage (Geng et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Photochemistry of nitric oxide and S-nitrosothiols in human skin

Pelegrino

Paganotti

Seabra

et al. 2020

Histochem Cell Biol

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Nitric oxide (NO) is related to a wide range of physiological processes such as vasodilation, macrophages cytotoxicity and wound healing. The human skin contains NO precursors (NO x). Those are mainly composed of nitrite (NO 2 −), nitrate (NO 3 −), and S-nitrosothiols (RSNOs) which forms a large NO store. These NO x stores in human skin can mobilize NO to blood stream upon ultraviolet (UV) light exposure. The main purpose of this study was to evaluate the most effective UV light wavelength to generate NO and compare it to each NO precursor in aqueous solution. In addition, the UV light might change the RSNO content on human skin. First, we irradiated pure aqueous solutions of NO 2 − and NO 3 − and mixtures of NO 2 − and glutathione and NO 3 − and S-nitrosoglutathione (GSNO) to identify the NO release profile from those species alone. In sequence, we evaluated the NO generation profile on human skin slices. Human skin was acquired from redundant plastic surgical samples and the NO and RSNO measurements were performed using a selective NO electrochemical sensor. The data showed that UV light could trigger the NO generation in skin with a peak at 280-285 nm (UVB range). We also observed a significant RSNO formation in irradiated human skin, with a peak at 320 nm (UV region) and at 700 nm (visible region). Pre-treatment of the human skin slice using NO 2 − and thiol (RSHs) scavengers confirmed the important role of these molecules in RSNO formation. These findings have important implications for clinical trials with potential for new therapies.

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“…Известно, что SLC9A1 -мембранный белок, переносящий Na + в клетку и H + из клетки -играет центральную роль в регуляции гомеостаза pH и является одним из основных источников кислотности в поверхностных слоях кожи [8]. Он также активируется наиболее частой мутацией при меланоме, BRAFV600E, тем самым вызывая нарушение регуляции pH во время инициации меланомы.…”

Section: сокращенияunclassified

Principal Components of Genetic Sequences: Correlations and Significance

Ефимов¹,

Efimov²,

Kovaleva³

et al. 2021

Math.Biol.Bioinf.

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Any numerical series can be decomposed into principal components using singular spectral analysis. We have recently proposed a new analysis method ‒ PCA-Seq, which allows calculating numerical principal components for a sequence of elements of any type. In particular, the sequence may be composed of nucleotide base pairs or amino acid residues. Two questions inevitably arise about interpretation of the obtained principal components and about the assessment of their reliability. For interpretation of the symbolic sequence principal components, it is reasonable to evaluate their correlations with numerical characteristics of the sequence elements. To assess the significance of correlations between sequences, one should bear in mind that standard significance criteria are based on the assumption of independence of observations, which, as a rule, is not fulfilled for real sequences. The article discusses the use of an anchor bootstrap technique for these purposes also previously developed by the authors of the article. In this approach it is assumed, that points of a metric space can represent the objects. When taken together they make up some fixed structure in it, in particular, a sequence. The objects are assigned the same random integer weights as in the classical bootstrap. This is sufficient to obtain the bootstrap distribution of the correlation coefficients and assess their significance. The coding sequence of the SLC9A1 gene (synonyms APNH, NHE1, PPP1R143) were taken as an example of use the anchor bootstrap technique in the genetic sequence analysis. Significant correlations of the first principal component were revealed with the hydrophobicity/“transmembraneity” of the corresponding fragments of the amino acid sequence, the phenylalanine content in them, as well as the difference in the T- and A-content in the corresponding nucleotide fragments. Earlier a similar pattern was found by other authors for other genes. Very likely, that it is of a more general nature.

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Cutaneous pH landscape as a facilitator of melanoma initiation and progression

Cited by 12 publications

References 188 publications

Probing the heterogeneous structure of eumelanin using ultrafast vibrational fingerprinting

Probing the heterogeneous structure of eumelanin using ultrafast vibrational fingerprinting

Photochemistry of nitric oxide and S-nitrosothiols in human skin

Principal Components of Genetic Sequences: Correlations and Significance

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