Cutaneous and systemic responses during primary and challenge infestations of sheep with the sheep scab mite, <i>Psoroptes ovis</i>

Broek, van den; Huntley,; Machell,; Taylor, Simon; Bates,; Groves, Mark D.; Miller, '

doi:10.1046/j.1365-3024.2000.00318.x

Cited by 50 publications

(47 citation statements)

References 28 publications

(37 reference statements)

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“…Infestation of the skin with P. ovis mites results in a rapid pro-inflammatory response within minutes of contact [16]. As such we focused our analyses on the first 24 hours post infestation to elucidate the signalling pathways involved in disease development.…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic analysis of the temporal host response to skin infestation with the ectoparasitic mite Psoroptes ovis

et al. 2010

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BackgroundInfestation of ovine skin with the ectoparasitic mite Psoroptes ovis results in a rapid cutaneous immune response, leading to the crusted skin lesions characteristic of sheep scab. Little is known regarding the mechanisms by which such a profound inflammatory response is instigated and to identify novel vaccine and drug targets a better understanding of the host-parasite relationship is essential. The main objective of this study was to perform a combined network and pathway analysis of the in vivo skin response to infestation with P. ovis to gain a clearer understanding of the mechanisms and signalling pathways involved.ResultsInfestation with P. ovis resulted in differential expression of 1,552 genes over a 24 hour time course. Clustering by peak gene expression enabled classification of genes into temporally related groupings. Network and pathway analysis of clusters identified key signalling pathways involved in the host response to infestation. The analysis implicated a number of genes with roles in allergy and inflammation, including pro-inflammatory cytokines (IL1A, IL1B, IL6, IL8 and TNF) and factors involved in immune cell activation and recruitment (SELE, SELL, SELP, ICAM1, CSF2, CSF3, CCL2 and CXCL2). The analysis also highlighted the influence of the transcription factors NF-kB and AP-1 in the early pro-inflammatory response, and demonstrated a bias towards a Th2 type immune response.ConclusionsThis study has provided novel insights into the signalling mechanisms leading to the development of a pro-inflammatory response in sheep scab, whilst providing crucial information regarding the nature of mite factors that may trigger this response. It has enabled the elucidation of the temporal patterns by which the immune system is regulated following exposure to P. ovis, providing novel insights into the mechanisms underlying lesion development. This study has improved our existing knowledge of the host response to P. ovis, including the identification of key parallels between sheep scab and other inflammatory skin disorders and the identification of potential targets for disease control.

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Section: Resultsmentioning

confidence: 99%

Transcriptomic analysis of the temporal host response to skin infestation with the ectoparasitic mite Psoroptes ovis

et al. 2010

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“…The disease presents as a type I hypersensitivity reaction with the release of serous exudate through the skin, which then dries to from the crusty scabs from which the disease takes its name e.g. [26]. The clinical signs of sheep scab include anorexia, weight loss, loss of fleece and pelt condition, and general loss of production.…”

Section: Introductionmentioning

confidence: 99%

“…The feasibility of vaccination is supported by the observation that prior infestation with the mite leads to a suppression of lesion development in subsequent infestations, indicative of a protective immune response [26]. Vaccination of sheep with soluble extracts of P .…”

Section: Introductionmentioning

confidence: 99%

Feeding-associated gene expression in sheep scab mites (Psoroptes ovis)

et al. 2009

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The mite Psoroptes ovis is the causative agent of sheep scab. Although not usually fatal, the disease can spread rapidly and is a serious animal welfare concern. Vaccine development against ectoparasites has primarily focussed on two sources of candidate vaccine antigens – “exposed” antigens that are secreted in saliva during feeding on a host and “concealed” antigens that are usually expressed in the parasite gut and may be involved in digestion. Here, we sought to identify genes encoding proteins important for mite feeding and digestion by a subtractive suppressive hybridisation approach comparing mRNA transcript abundance in “fed” and “starved” mites. The study identified a variety of genes which are up-regulated by feeding mites. These included group 1, 5, 7 and 13 allergens including the previously described cysteine protease Pso o 1. In addition, numerous novel genes were identified here including some encoding potential salivary gland proteins and others encoding proteins which may facilitate feeding such as a serum opacity factor. An olfactory receptor-like protein was identified in the starved mite population which may help the mite to identify a host.

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“…In cattle infected with D. hominis larvae basophils and monocytes were not expressive 2 . Basophilia occurs in ectoparasitoses such as sheep scab 22 and tick 21 .…”

Section: Discussionmentioning

confidence: 99%

Expression of circulating leucocytes before, during and after myiasis by Dermatobia hominis in experimentally infected rats

Gonçalves

Pereira

Evangelista

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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SUMMARYExpression of circulating white blood cells was investigated in rats (Rattus norvegicus) experimentally infected with larvae of Dermatobia hominis, the human bot fly. Leucocytes were counted prior to infection (control group) as well as at 6, 10, 15, 20 and 28 days post-infection (dpi) and at 7, 15, 30 and 60 days post-larval emergence (dple). Total leucocyte numbers did not differ markedly among the groups. Significant differences were registered when values from control and animals harboring each larval stage of D. hominis were compared; with crescent rank: L 1 -, L 2 -, control and L 3 -infected groups. Leucocyte numbers were significantly higher in the control, 15, 20 or 28 dpi groups than in the 6 dpi animals. Higher counts were observed in control, L 2 -or L 3 -infected rats than L 1 -infected animals. Neutrophils, eosinophils and both large and small lymphocytes were also counted and analyzed. Basophils and monocytes were insufficient in number to permit statistical studies. These results stimulate the continuity of the studies about the host-parasite relationship in the dermatobiosis.

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Cutaneous and systemic responses during primary and challenge infestations of sheep with the sheep scab mite, Psoroptes ovis

Cited by 50 publications

References 28 publications

Transcriptomic analysis of the temporal host response to skin infestation with the ectoparasitic mite Psoroptes ovis

Transcriptomic analysis of the temporal host response to skin infestation with the ectoparasitic mite Psoroptes ovis

Feeding-associated gene expression in sheep scab mites (Psoroptes ovis)

Expression of circulating leucocytes before, during and after myiasis by Dermatobia hominis in experimentally infected rats

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