Cushioned versus noncushioned centrifugation: Sperm recovery rate and integrity

Len, Jose; Beehan, D.; Lyle, Sara K.; Eilts, B.E.

doi:10.1016/j.theriogenology.2013.06.009

Cited by 8 publications

(8 citation statements)

References 19 publications

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“…Studies in different species were carried out, particularly in humans, to investigate the effects of such techniques on sperm cells quality [48], though few studies have been performed to date to clarify whether centrifugation "per se" could impair sperm quality. Studies reporting the effects of centrifugation on equine spermatozoa have been carried out for decades and findings are controversial [24][25][26][27][28][29][30].…”

Section: Discussionmentioning

confidence: 99%

“…This is mainly due to osmotic stress and exposure to deep temperatures [18][19][20][21][22][23]. In equine sperm, different studies on the effects of centrifugation were performed, reporting contrasting results [24][25][26][27][28][29][30]. Although these studies investigated the effects of centrifugation on sperm motility, the effects on mitochondrial functionality and on different oxidative parameters have not been reported.…”

Section: Introductionmentioning

confidence: 99%

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Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm

Marzano

Moscatelli

Giacomo

et al. 2020

Biology

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Conventional sperm selection techniques used in ARTs rely on centrifugation steps. To date, the different studies reported on the effects of centrifugation on stallion sperm motility provided contrasting results and do not include effects on mitochondrial functionality and different oxidative parameters. The effects of different centrifugation protocols (300× g for 5′, 300× g for 10′, 1500× g for 5′ and 1500× g for 10′ vs. no centrifugation) on motility and oxidative status in cryopreserved stallion sperm, were analyzed. After centrifugation, almost all motility parameters were significantly altered, as observed by computer-assisted sperm analysis. A polarographic assay of oxygen consumption showed a progressive decrease in mitochondria respiration from the gentlest to the strongest protocol. By laser scanning confocal microscopy, significant reduction of mitochondrial membrane potential, at any tested protocol, and time-dependent effects, at the same centrifugal force, were found. Increased DNA fragmentation index at any tested protocol and time-dependent effects at the same centrifugal force were found, whereas increased protein carbonylation was observed only at the strongest centrifugal force. These results provide more comprehensive understandings on centrifugation-induced effects on cryopreserved stallion sperm and suggest that, even at a weak force for a short time, centrifugation impairs different aspects of equine sperm metabolism and functionality.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm

Marzano

Moscatelli

Giacomo

et al. 2020

Biology

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“…During cushioned centrifugation, the use of a dense cushion, mainly iodixanol, in the bottom of the centrifugation tube was used to avoid the excessive compaction of the sperm pellet during high speed centrifugation [ 43 ]. The data reported in literature showed that a centrifugation speed exceeding 1,800 × g could be deleterious for equine sperm motility [ 44 ], while centrifugation at 600 × g [ 45 ], 900 × g [ 44 ], and 1,000 × g [ 43 ] had a negligible effect on equine sperm characteristics. However, the data reported in the present study showed that the presence of a cushion (DLC) did not improve the seminal quality either after centrifugation, or after equilibration or cryopreservation, compared with SLC.…”

Section: Discussionmentioning

confidence: 99%

Single and double layer centrifugation improve the quality of cryopreserved bovine sperm from poor quality ejaculates

Gloria

Carluccio

Wegher

et al. 2016

J Animal Sci Biotechnol

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BackgroundDensity gradient centrifugation was reported as a technique of semen preparation in assisted reproductive techniques in humans and animals. This technique was found to be efficient in improving semen quality after harmful techniques such as cryopreservation. Recently a modified technique, single layer centrifugation, was proposed as a technique providing a large amount of high quality spermatozoa, and this treatment was performed before conservation. Single layer centrifugation has been studied prevalently in stallions and in boars, but limited data were available for bulls. Occasionally bulls are known to experience a transient reduction in semen quality, thus techniques that allow improvement in semen quality could be applied in this context. The aim of this study was the evaluation of single layer and double layer centrifugation by the use of iodixanol, compared with conventional centrifugation and non-centrifuged semen, on the sperm characteristics during the cryopreservation process in bulls with normal and poor semen quality.ResultsSingle layer centrifugation and double layer centrifugation both significantly increased the percentage of normal spermatozoa and decreased the percentage of non-sperm cells in poor quality samples, while both were ineffective in those of normal quality. Sperm characteristics in poor quality samples increased after single layer centrifugation and double layer centrifugation, reaching values similar to those recorded in normal samples, and this trend is maintained after equilibration and after cryopreservation. On the other hand, SLC and DLC resulted in a consistent reduction in the spermatozoa recovered, and this resulted in a reduction of the absolute amount of spermatozoa cryopreserved in the normal samples, without a clear improvement in sperm characteristics in this type of sample.ConclusionsThese data suggested that both SLC and DLC could be performed in practice, but their application should be limited to the cases in which the quality of the spermatozoa recovered is more important than the total amount of spermatozoa.

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“…Evidence has suggested that the recovery rate of sperm cells after centrifugation is related to the g-force applied, however an excessive centrifugation force reduced the viability of spermatozoa, probably because of the physical pressure against the wall of the tube [20,21]. The data reported in the literature showed that a centrifugation speed exceeding 1800×g could be deleterious for equine sperm motility [22], while centrifugation at 600×g [23], 900×g [22], and 1000×g [24] had a negligible effect on equine sperm characteristics. In a study conducted in the bull, single (SLC) and double layer centrifugation (DLC) were performed effectively to improve the quality of the semen samples.…”

Section: Introductionmentioning

confidence: 99%

Cushioned and High-Speed Centrifugation Improve Sperm Recovery Rate But Affect the Quality of Fresh and Cryopreserved Feline Spermatozoa

Gloria,

Cunto,

Zambelli

et al. 2023

Preprint

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Cushioned versus noncushioned centrifugation: Sperm recovery rate and integrity

Cited by 8 publications

References 19 publications

Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm

Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm

Single and double layer centrifugation improve the quality of cryopreserved bovine sperm from poor quality ejaculates

Cushioned and High-Speed Centrifugation Improve Sperm Recovery Rate But Affect the Quality of Fresh and Cryopreserved Feline Spermatozoa

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