Soil erosion is a major environmental problem that affects people's livelihoods and environmental health, particularly in developing countries. The present study aimed to identify soil erosion hotspot areas and prioritization in the Anger River Sub-basin for conservation planning. The Revised Universal Soil Loss Equation model and geospatial technologies were adopted to identify soil erosion hotspot areas and prioritization of the sub-watershed for conservation planning. Key parameters such as rainfall data, soil data, slope length and steepness factor, cover management, and conservation practices were used to estimate potential soil erosion risk in the sub-watershed. The results showed that the annual soil loss rate in the Anger River Sub-basin ranged between 0 and 932.6 t/ha/year with a mean annual soil loss of 83.7 t/ha/year. About 1,140.7 km2 (43.6%) and 220.6 km2 (8.4%) were categorized under very severe and severe soil loss types, respectively. The mid- and upstream areas, as well as the steeper parts of the sub-watershed, were highly exposed to soil erosion. This research provides tangible evidence in the decision-making process for soil and water conservation practices at a sub-watershed scale. Moreover, further research should be conducted at a micro-watershed scale to minimize the effects of soil erosion on the health and sustainability of the watershed.