Current status, scope and constraints of sexed semen - An Indian perspective

Kumar, Amarjeet; Vineeth, M R; Sinha, Rekha; Singh, Raushan Kumar; Thakur, Abhishek; Gupta, Shailesh Kumar

doi:10.18805/ar.v0i.11286

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…However, as reported by Briano-Rodriguez et al [2] a prevalence of 2.61 % of CD carriers was found in a population sample of HF calves from different Regions of Uruguay. These results were similar to those observed for India (1.67 %, [15]) and the USA (2.6 %, [3]), but lower than those reported for other Countries. For example, in Germany, Kipp et al [13] calculated the frequency of HF calves born homozygous for the CD haplotype in approximately 8.7 % of 3,400 calves born each year.…”

Section: Resultssupporting

confidence: 89%

Optimization of Real-Time PCR-melting for detection of the Cholesterol-deficiency mutation in Holstein Friesian cattle

et al. 2021

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The purpose of this study was to optimize a real-time PCR-melting analysis for reliable and economical detection of the 7.5 Kb mutant insert of the BoERVK bovine transposable element in exon 5 of the Apolipoprotein B (APOB) gene, which causes cholesterol deficiency — CD — (OMIA 001965-9913). This technique was also used to perform a preliminary molecular screening to detect this mutation in a DNA sample of Holstein Friesian cows (HFc) of six commercial dairy farms from different regions of Uruguay. By amplifying the 170 and 146 bp PCR products, two genotypes were clearly identified: homozygote (wild type wt/wt) and heterozygote (carrier of the CD mutation: MUT/wt). The homozygous wt/wt genotype was detected in the representative sample of 103 HFc. It is concluded that Real-Time PCR-melting analysis is a fast, easily interpretable, low cost, and highly accurate technique for detecting this mutation, which can be implemented in genetic selection programs to prevent the spread of the disease in HFc

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Section: Resultssupporting

confidence: 89%

Optimization of Real-Time PCR-melting for detection of the Cholesterol-deficiency mutation in Holstein Friesian cattle

et al. 2021

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“…Using this sexed semen, the rates of pregnancy observed for cows were 20.7% and for heifers, 35.3%. The National Dairy Research Institute (NDRI), Karnal, has also been working for cattle semen sexing to multiply the country's indigenous and crossbred cows by providing sexual semen to farmers (Kumar et al 2016). The animal husbandry department plans to breed 60 lakh cows each year, through the sexed semen, to develop the cattle breed.…”

Section: Current Status Of Sperm Sexing In India and Future Prospectivementioning

confidence: 99%

Use of sexed semen for genetic improvement of indigenous dairy cattle and buffaloes productivity

Yata

Singh

Kumar

et al. 2022

Indian J of Anim Sci

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The productivity of dairy animals in India needs to be improved to feed the growing population in the decades ahead. Sperm sexing offer a promising breeding strategy to meet the increased demand for food production and nutritional protection. Application of sexed sperm in the breeding of indigenous cattle and buffalo would help to protect endangered species, and it also helps to improve the productivity of indigenous milch animals which can survive for long starvation and well adopted by Indian climatic conditions with disease resistance. Â The productivity of crossbreed milch animals also required to meet the food demand associated with population growth. This review discusses the sustainability of Indian dairy animals and provides sperm sexing strategies to boost dairy animal production in India.

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“…The difference in DNA content of X and Y forms the basis of flow cytometric analysis of DNA content in X and Y [2,16] The difference in DNA content in domestic animals between X and Y chromosomes bearing spermatozoa ranges from (3-4.5)% [17] According to Kumar et.al. [18], Flow Cytometry uses Flourescent dye that stains DNA fluorescent [19,20] It uses Flourescent Dye that stains the DNA OF the spermatozoa . It is based on the difference of DNA in X and Y chromosomes.…”

Section: Flow Cytometrymentioning

confidence: 99%

Current Status of Sex Sorted Semen and its Long Term effect on Population Dynamics and Y-Chromosome Degeneration of the Breed Among Dairy Animals in Jharkhand, India: A Review

Kumari

Prasad

Pandey³

et al. 2021

CJAST

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Sex Sorted Semen gives the liberty of producing offspring of the desired sex - in farming animals by using it in conjunction with other assisted reproductive technologies such as Artificial Insemination and In-Vitro Fertilization after selecting the healthy sperm and separating into X-Female and Y-male Chromosome bearing populations based on their DNA content. It is an important biotechnological tool to increase the milk production and the profitability of Dairy Industry. Current study deals with the Principle, methods, main method, advantages, disadvantages and the current status of Sex sorted semen in India and Jharkhand. The main emphasis of this study is to draw the attention of Scientific fraternity towards the effect of Sex Sorted Semen on Population dynamics. The Sex Sorted semen increases the deviation of ratio between Male and Female Population from ideal 1:1, thereby decreasing the effective population size Ne and thus slowly reducing the viability and survivability of the population or breed concerned. Further the already depleting Y chromosomes will be reaped off all its genes in long run at a faster rate due to antagonistic selection pressure arising out of Artificial selection via Sex Sorted Semen acting against all the gene of Y chromosome of the breed or population concerned which might disturb many vital genes and the associated functions. The degeneration and extinction of scientists have been predicted long ago. The effect of Sex Sorted semen on Y- chromosome degeneration is yet to be pointed out, calculated and subsequently verified in any of the literatures. SSS is indeed a boon for India as well as Jharkhand. It might be too early to predict about the negative impact of SSS on population dynamics and Y-Chromosome degeneration. Further research work must be done to assess the extent and authencity of above mentioned impact( Predicted theoretically) by calculation as well as practical field based Experimentation.

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Current status, scope and constraints of sexed semen - An Indian perspective

Cited by 6 publications

References 16 publications

Optimization of Real-Time PCR-melting for detection of the Cholesterol-deficiency mutation in Holstein Friesian cattle

Optimization of Real-Time PCR-melting for detection of the Cholesterol-deficiency mutation in Holstein Friesian cattle

Use of sexed semen for genetic improvement of indigenous dairy cattle and buffaloes productivity

Current Status of Sex Sorted Semen and its Long Term effect on Population Dynamics and Y-Chromosome Degeneration of the Breed Among Dairy Animals in Jharkhand, India: A Review

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