Current status of genome editing in vector mosquitoes: A review

Reegan, Appadurai Daniel; Ceasar, Stanislaus Antony; Paulraj, Michael Gabriel; Ignacimuthu, S.; Al-Dhabi, Naïf Abdullah

doi:10.5582/bst.2016.01180

Cited by 29 publications

(21 citation statements)

References 71 publications

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“…Zinc finger nuclease (ZFN) technique is a genome editing method that is based on the specificity of DNA binding to the zinc finger nuclease. It is a target-specific that is engineered to bind and cleave with DNA at needed sites of the genome (Reegan, et al, 2016). The system comprises of DNA binding domain in association with zinc finger which capable of recognising the specific sequence on the genome and nuclease domain made up of FokI enzyme that cleaves the specific site of the DNA (Reegan et al, 2016).…”

Section: Zinc Finger Nucleases (Zfns)mentioning

confidence: 99%

“…It is a target-specific that is engineered to bind and cleave with DNA at needed sites of the genome (Reegan, et al, 2016). The system comprises of DNA binding domain in association with zinc finger which capable of recognising the specific sequence on the genome and nuclease domain made up of FokI enzyme that cleaves the specific site of the DNA (Reegan et al, 2016). The DNA binding domain of individual ZFNs characteristically is made up of three to six individual zinc finger repeats with each finger capable of recognizing three base pairs.…”

Section: Zinc Finger Nucleases (Zfns)mentioning

confidence: 99%

“…The transcription activator-like effector nucleases (TALEN) is somewhat alike with the Zinc finger nuclease (ZFN) technique only that the former is easier compared to the later and more specific to target sequence (Reyon, 2012). The DNA binding domain of TALEN comprises of a highly conserved repeat of 33-34 amino acid sequence with a difference at the 12th and the 13th amino acids with the two positions exhibiting variation in terms of their positioning and shows a powerful correlation with specific nucleotide recognition in the genome (Reegan, et al, 2016). The relationship existing between amino acid sequence and DNA recognition has enabled the engineering of the specific DNA-binding domains.…”

Section: Transcription Activator-like Effector Nucleases (Talens)mentioning

confidence: 99%

“…12, p. 27-38. (Reegan, et al, 2016). The system is much easier and convenient to handle than those of the ZFN and TALEN.…”

Section: Clustered Regulatory Interspaced Short Palindromic Repeats (mentioning

confidence: 99%

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Bioengineering of malaria vectors, anopheles mosquitoes (Diptera: Culicidae) as a control strategy: An overview

Ileke¹,

Olabimi²

2019

Braz. J. Biol. Sci.

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Disease in any form is undesirable in any human population. Malaria is a communicable disease that is vectored by female Anopheles mosquitoes. It is the leading vector transmitted disease in terms of the number of morbidity and mortality accounting for over 200 million cases annually. Several control measures have been employed by man over the years to control the vector which will in turn lead to the control of the diseases with the popular ones involving the use of insecticidal nets and indoor residual spraying of insecticides. However, these control measures have their various pitfalls. The use of genetically modified mosquitoes (GMMs) through bioengineering may be a promising method of reducing malaria vector population in our environment. This process involves population replacement technique (PRT) and population suppression techniques (PST). With proper integration of GMMs into the already existing control measures employed in the management of mosquitoes, a remarkable decrease in the prevalence of malaria is envisaged.

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Section: Zinc Finger Nucleases (Zfns)mentioning

confidence: 99%

Section: Zinc Finger Nucleases (Zfns)mentioning

confidence: 99%

Section: Transcription Activator-like Effector Nucleases (Talens)mentioning

confidence: 99%

“…12, p. 27-38. (Reegan, et al, 2016). The system is much easier and convenient to handle than those of the ZFN and TALEN.…”

Section: Clustered Regulatory Interspaced Short Palindromic Repeats (mentioning

confidence: 99%

See 2 more Smart Citations

Bioengineering of malaria vectors, anopheles mosquitoes (Diptera: Culicidae) as a control strategy: An overview

Ileke¹,

Olabimi²

2019

Braz. J. Biol. Sci.

View full text Add to dashboard Cite

show abstract

“…Our ability to understand the molecular basis of mosquito behavior has been enhanced by the recent development of genome editing tools such as CRISPR-Cas9 RNA-guided nucleases, TALE-effector Nucleases (TALENS) and Zinc Finger Nucleases (ZFNs) in Anopheles and Aedes [8]. These approaches can be employed to facilitate targeted mutagenesis at any gene of interest to determine their contribution to host detection and blood feeding.…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis of mosquito detection of humans

Raji

DeGennaro

2017

Current Opinion in Insect Science

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Mosquitoes detect the presence of humans by integrating chemosensory, thermal, and visual cues. Among these, odors are crucial for mosquito host detection. Insects have evolved a diverse repertoire of receptors to detect their plant and animal hosts. Genetic analysis of these receptors in Drosophila has set the stage for similar studies in mosquitoes. The diversity of the cues involved in mosquito host-seeking has made designing behavioral control strategies a challenge. The sensory receptors that are most important for mosquito detection of humans can now be determined using genome editing. Here, we will review our current understanding of the salient cues that attract mosquitoes, their receptors, and suggest ways forward for novel olfaction-based vector control strategies.

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Three synthetic biology applications and their paths to impact in Australia: Cane toads, bacteriophages, and biomining microbes

Carter

Mankad

Hobman

et al. 2022

Biotechnology Journal

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Synthetic biology [synbio] applications have the potential to assist in addressing significant global health and environmental challenges. Australian research institutes are investing in formative research to develop synbio technologies capable of meeting these challenges. Alongside the laboratory research, investigating the broader social, institutional, and ethical considerations that synbio presents has been a priority. We conducted targeted qualitative research to uncover the barriers and opportunities for a range of multisectoral stakeholders identified as potential end-users of the science under development. The research provides insights into the research implementation environment for three synthetic biology applications: (1) gene editing cane toads (Rhinella marina) to reduce their environmental impact; (2) engineering bacteriophages to combat antimicrobial resistance in humans; and (3) engineering microbes to improve biomining efficiency in the mining industry. In-depth interviews (N = 23) with government, research and civil society representatives revealed key challenges in the impact pathway for each application. The strongest themes uncovered during interviews related to perceived negative public attitudes towards genetic technologies, a lack of investment in critical research infrastructure, unclear regulatory pathways and the presence of a strong social and environmental imperative underpinning technology development. These findings reveal specific entry points for further engagement with the most immediate end-users of synbio. Separate from research on public attitudes to synbio, the cases highlight the various hurdles to achieving research impact, according to experts who will likely use, approve or invest in these applications in the future. The themes uncovered inform avenues for strengthening engagement and research coordination in Australia and elsewhere.

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Current status of genome editing in vector mosquitoes: A review

Cited by 29 publications

References 71 publications

Bioengineering of malaria vectors, anopheles mosquitoes (Diptera: Culicidae) as a control strategy: An overview

Bioengineering of malaria vectors, anopheles mosquitoes (Diptera: Culicidae) as a control strategy: An overview

Genetic analysis of mosquito detection of humans

Three synthetic biology applications and their paths to impact in Australia: Cane toads, bacteriophages, and biomining microbes

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