Current status in production and utilization of dihaploids from somatic hybrids between eggplant (Solanum melongena L.) and its wild relatives

Rotino, Giuseppe Leonardo; Sihachakr, D.; Rizza, Fulvia; Valè, Giampiero; Tacconi, M. G.; Alberti, P.; Mennella, G.; Sabatini, E.; Toppino, L.; D’Alessandro, Antonietta; Acciarri, N.

doi:10.1007/s11738-005-0077-4

Cited by 32 publications

(17 citation statements)

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“…By the end of this decade, other reports published the production of haploid eggplant individuals (Isouard et al 1979). Misra et (Rotino 1996;Rotino et al 2005). Genetic variability was also observed in plants regenerated from anther cultures (Rotino et al 1991).…”

Section: Androgenic Dhs In Eggplantmentioning

confidence: 93%

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Androgenesis in recalcitrant solanaceous crops

et al. 2010

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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 2 Abstract Tomato, eggplant and pepper are three solanaceous crops of outstanding importance worldwide. Thus, a fast and cheap method to produce pure (homozygous) lines is a priority for breeders. Traditionally, pure lines are produced by classical inbreeding and selection techniques, which are time consuming (several years) and costly. Alternatively, it has become possible to accelerate the production of homozygous lines through a biotechnological approach: the induction of androgenesis to generate doubled haploid (homozygous) plants. This biotechnological in vitro tool reduces the process to only one generation, which implies important time and costs savings. These facts make androgenic doubled haploids the choice in a number of important crops where the methodology is well set up. Unfortunately, recalcitrant solanaceous crops such as tomato, eggplant and pepper are still far from an efficient and reliable technology to be applied to different genotypes in breeding programs on a routine basis. In eggplant and pepper, only anther cultures are known to work relatively well. Unfortunately, a more efficient and promising technique, the culture of isolated microspores, is not sufficiently developed yet. In tomato, none of these methods is available. However, recent advances in the knowledge of zygotic and androgenic embryo development are filling the gaps and opening new ways to achieve the final goal of an efficient protocol in these three recalcitrant species. In this review, we outline the state of the art on androgenic induction in tomato, eggplant and pepper, and postulate new experimental ways in order to overcome current limitations.

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Section: Androgenic Dhs In Eggplantmentioning

confidence: 93%

“…At present, the Dumas de Vaulx method is the basis of many protocols, now routine methods used in breeding programs for the production of pure DH lines, adapted to particular cultivars ( Figure 2; Sanguineti et al 1990;Rotino 1996;Rizza et al 2002;Rotino et al 2005; http://www.scri.sari.ac.uk/assoc/COST851/Default.htm).…”

Section: Androgenic Dhs In Eggplantmentioning

confidence: 99%

Androgenesis in recalcitrant solanaceous crops

et al. 2010

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“…During a backcross process, DH individuals can be obtained at different generations, together with marker-assisted selection, to help fix the introgressed character (Belicuas et al 2007;Toojinda et al 1998). In somatic interspecific hybrids between crop species and their wild relatives, where often sterile and tetraploids individuals are obtained, anther culture can be used to bring back the ploidy level to the diploid status (Rotino et al 2005).…”

Section: Dhs As An Assisting Tool For Plant Breedingmentioning

confidence: 99%

“…They are also used as a tool to assist research, for example to assess gene exchanges (Toppino et al 2008) and to reduce the ploidy of tetraploid somatic hybrids between common eggplant and wild relatives (Rizza et al 2002;Rotino et al 2005). Anther culture, however, has several limitations, including the possible occurrence of somatic regenerants derived from anther walls, the uncontrolled contribution of tapetal cells to culture conditions, and the general low efficiency of the technique (Seguí-Simarro 2015;Seguí-Simarro 2016;Seguí-Simarro et al 2011b).…”

Section: First Blockmentioning

confidence: 99%

“…They are also used as a tool to assist research, for example to assess gene exchanges (Toppino et al 2008) and to reduce the ploidy of tetraploid somatic hybrids between common eggplant and wild relatives (Rizza et al 2002;Rotino et al 2005). However, eggplant is still considered recalcitrant to microspore culture because, although microspore-derived embryos are initially formed, they do not complete embryogenesis and transform into calli (CorralMartínez and Seguí-Simarro 2012), making it mandatory additional steps to regenerate DH plants from these calli through organogenesis .…”

Section: Introductionmentioning

confidence: 99%

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Calcium and cell wall dynamics during microspore embryogenesis and double haploid production in rapeseed and eggplant

Sendra¹

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Thesis layout and cover design: Alba Rivas Sendra and André Arôxa Watercolor drawings: Alba Rivas Sendra AcknowledgementsEn primer lugar, quiero dar las gracias especialmente a Jose María, que me ha guiado en este proceso desde el primer momento. Me ha impulsado a crecer y, cuando no era posible, me ha traído un banquito para que pudiera alcanzar siempre más alto. Con tu ayuda me he convertido en una investigadora y todo lo que he aprendido me acompañará para siempre. Aunque a veces ponernos de acuerdo ha sido un reto, eso representa la esencia misma de la investigación y la ciencia, y lo agradezco sinceramente. Los retos siempre nos inspiran, nos obligan a aprender, a mantenernos activos y a tener menos miedo de las soluciones creativas. Y de esto es mejor tener los bolsillos llenos.También quiero expresar mi sincero agradecimiento a Kim Boutilier. Ella me abrió sus puertas el primer día, y las mantiene abiertas desde entonces. No solo aprendí cosas nuevas de los mejores expertos, sino que la estancia en su laboratorio me permitió ver un mundo diferente, encontrar un segundo hogar, sembrar y recoger muchísimo amor expatriado increíblemente verdadero, y darme cuenta de que son las cosas más diminutas (como puede ser una pequeña bici rosa) las que llenan nuestras vidas de felicidad.Familia, amigos, amigos que se convierten en familia, todos habéis tenido un papel en la parte de mí que queda reflejada en este libro. Los que siempre habéis caminado a mi lado, los que aparecisteis un día y ya nunca os marchasteis, los que acabáis de llegar pero es como si siempre hubieseis estado aquí, los que me dais la bienvenida cuando vuelvo a casa, donde quiera que mi casa esté, e incluso aquellos que solo me acompañaron durante una etapa... Todos vosotros habéis ayudado a dar forma a la persona que soy hoy. No hay un momento del que me arrepienta, un beso que desee nunca haber dado, una palabra que preferiría no haber oído. Ha sido un largo camino, pero esto es solo el comienzo. Las olas siguen llegando a la playa, las células siguen creciendo en nuestras placas de cultivo, y la vida, como el espectáculo, debe continuar. Lo único importante es ser feliz. Siempre hacia delante y apuntando alto, retroceder nunca, rendirse jamás (Corey Yuen, 1986).First of all, I specially want to thank Jose María, who has guided me through this life-changing process since the very beginning. He encouraged me to grow, and when it was not possible, he brought me a stool so I could always reach higher.With your help I became a researcher, and everything I learnt will be with me forever. Even though sometimes it was challenging to come to an agreement, this represents the core essence of research and science, and I truly appreciate it.Challenges always inspire us, make us learn, keep us active and take away our fear of creative solutions. And it is always better to have plenty of these.I also want to express my sincere thanks to Kim Boutilier. She opened her door to me on the first day, and still holds it open. Not only have I learnt new things from...

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Plant Interspecific Hybridization: Outcomes and Issues at the Intersection of Species

Morgan

Timmerman-Vaughan

Conner

et al. 2010

Plant Breeding Reviews

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Current status in production and utilization of dihaploids from somatic hybrids between eggplant (Solanum melongena L.) and its wild relatives

Abstract: Cur rent sta tus in pro duc tion and uti li za tion of dihaploids from so matic hy brids be tween egg plant (Solanum melongena L.) and its wild rel a tives C R A

Cited by 32 publications

References 14 publications

Androgenesis in recalcitrant solanaceous crops

Androgenesis in recalcitrant solanaceous crops

Calcium and cell wall dynamics during microspore embryogenesis and double haploid production in rapeseed and eggplant

Plant Interspecific Hybridization: Outcomes and Issues at the Intersection of Species

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