Current state of the art in peptide-based gene delivery

Hadianamrei, Roja; Zhao, Xiaoyong

doi:10.1016/j.jconrel.2022.02.010

Cited by 48 publications

(45 citation statements)

References 150 publications

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“…130 However, a key disadvantage associated with magnetofection is the agglomeration of MNPs, which can result following the removal of the external magnetic field. 135…”

Section: Materials Advances Reviewmentioning

confidence: 99%

Therapeutic applications of magnetic nanoparticles: recent advances

Roy

Kritika

2022

Mater. Adv.

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“…130 However, a key disadvantage associated with magnetofection is the agglomeration of MNPs, which can result following the removal of the external magnetic field. 135…”

Section: Materials Advances Reviewmentioning

confidence: 99%

Therapeutic applications of magnetic nanoparticles: recent advances

Roy

Kritika

2022

Mater. Adv.

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“…25 Peptides are also limited in pulmonary delivery due to their low molecular weight and rapid degradation under physiological conditions. 26 Other materials like polymeric nanoparticles, dendrimers, and micelles have disadvantages like poor reproducibility, uneven size distribution, and difficulty in production and commercialization. 27 Therefore, materials with good reproducibility, environmentally and economically friendly production, and viable commercialization for pulmonary delivery are also becoming increasingly important.…”

Section: Introductionmentioning

confidence: 99%

“…Even though exosomes possess inherent advantages over exogenous carriers in terms of biocompatibility, their purification techniques are cumbersome and expensive for large-scale manufacturing . Peptides are also limited in pulmonary delivery due to their low molecular weight and rapid degradation under physiological conditions . Other materials like polymeric nanoparticles, dendrimers, and micelles have disadvantages like poor reproducibility, uneven size distribution, and difficulty in production and commercialization .…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Biodegradable Covalent Cyclodextrin Frameworks as Particulate Carriers for Inhalation Therapy against Acute Lung Injury

Sun

et al. 2022

ACS Appl. Mater. Interfaces

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Drug therapies for acute lung injury (ALI) are far from satisfactory, primarily because drugs cannot specifically target the lungs. Direct delivery of drugs to the deep alveolar regions by inhalation administration is crucial for the treatment of ALI. However, conventional inhalable carriers such as lactose and mannitol are generally inactive. Therefore, the use of a novel pharmacologically active carrier for pulmonary delivery may produce synergetic effects in treating ALI. Considering the pathophysiological environment of ALI, which typically featured excessive reactive oxygen species (ROS) and acute inflammation, we synthesized a novel kind of biodegradable and ROS-sensitive cross-linked covalent cyclodextrin frameworks (OC-COF) with uniform inhalable particle size to treat ALI. OC-COF was devised to incorporate H 2 O 2 -scavenging peroxalate ester linkages, which could hydrolyze and eliminate ROS generated in inflammatory sites. Ligustrazine (LIG), an antioxidant and anti-inflammatory natural compound, was loaded into OC-COF and evaluated as a dry powder inhaler (LIG@OC-COF) in vitro and in vivo, showing favorable aerodynamic properties and prominent antioxidant and anti-inflammatory capacities for the synergistic effects of OC-COF and LIG. In ALI rats, inhalation of LIG@OC-COF with a one-fifth LIG dose significantly alleviated the inflammation, oxidant stress, and lung damage. Western blot analysis demonstrated that LIG@OC-COF protected the lungs by regulating the Nrf2/NF-κB signaling pathway. In summary, this study provides a novel ROS-responsive material as an inhalable particulate carrier for the improved treatment of ALI and other medical conditions.

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“…By having merits of good biocompatibility, inherent biodegradability, excellent assembly capacity, ease of modification, and stimuli responsibility, peptides are promising candidates for gene delivery by condensing nucleic acid drugs and facilitating their cellular uptake, internalization, and gene expression. 4 Peptides can be rationally designed based on the key structural features for interacting with nucleic acid drugs so as to enhance their potential for being used in gene-based therapeutic applications. 5 Two strategies are available to achieve peptidemediated gene delivery, that is, covalent bonding and noncovalent assembly.…”

Section: Introductionmentioning

confidence: 99%

“…Peptides, composed of approximately 50 or fewer amino acid monomers, have attracted considerable attention as therapeutics for biomedical applications. By having merits of good biocompatibility, inherent biodegradability, excellent assembly capacity, ease of modification, and stimuli responsibility, peptides are promising candidates for gene delivery by condensing nucleic acid drugs and facilitating their cellular uptake, internalization, and gene expression . Peptides can be rationally designed based on the key structural features for interacting with nucleic acid drugs so as to enhance their potential for being used in gene-based therapeutic applications .…”

Section: Introductionmentioning

confidence: 99%

Designed Peptide Assemblies for Efficient Gene Delivery

Cao

2022

Langmuir

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The safe and efficient delivery of nucleic acids including DNA, mRNA, siRNA, and miRNA into targeted cells is critical for gene therapy. Currently, viral gene vectors are very popular, but they have potential toxicity and insecurity. Therefore, the development of nonviral vectors has attracted considerable research attention. Peptide assemblies are superior candidates for being used as gene vectors by having good biocompatibility, versatile molecular design, excellent assembly capacity, ease of modification, and stimuli responsivity. The de novo designed peptides not only can induce efficient condensation of nucleic acids into compacted nanoparticles and protect them from enzymatic digestion but also can effectively overcome biological barriers and improve gene delivery efficiency through targeted delivery, enhanced cellular uptake, improved endolysosomal escape, and nuclear importation. By having these merits, peptidic gene vectors are developing fast, showing outstanding advantages compared to liposome and polymer vectors. This Perspective focuses on peptidic gene delivery systems by emphasizing the molecular design strategies for meeting the criteria of gene condensation, protection from nuclease degradation, cellular uptake, endolysosomal escape, and so on. The new arising research area of peptide-based artificial viruses for gene and ribonucleoprotein delivery has also been reviewed. The challenges and future perspectives are put forward, aiming to provide a conclusive guide for the development of peptidic delivery systems to achieve efficient gene therapy.

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Current state of the art in peptide-based gene delivery

Cited by 48 publications

References 150 publications

Therapeutic applications of magnetic nanoparticles: recent advances

Therapeutic applications of magnetic nanoparticles: recent advances

Antioxidant Biodegradable Covalent Cyclodextrin Frameworks as Particulate Carriers for Inhalation Therapy against Acute Lung Injury

Designed Peptide Assemblies for Efficient Gene Delivery

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