Current research on acute phase proteins in veterinary diagnosis: an overview

Murata, Hideo; Shimada, Nobuaki; Yoshioka, Miyako

doi:10.1016/s1090-0233(03)00119-9

Cited by 900 publications

(982 citation statements)

References 163 publications

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“…The acute phase protein, haptoglobin, was considered to be a useful measure of inflammatory and stress responses in cattle (Murata et al, 2004). Haptoglobin concentration did not increase, but did decrease, after the initiation of gradual weaning in this study.…”

Section: Figurementioning

confidence: 45%

Characterisation of haematological profiles and whole blood relative gene expression levels in Holstein-Friesian and Jersey bull calves undergoing gradual weaning

Johnston

Kenny

Kelly

et al. 2016

animal

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Haematological profiles indicate the health status of an animal and can be used to identify sub-clinical stress responses. The objectives of the study were to examine (i) the effect of breed and plane of nutrition, on haematological profiles of artificially reared Holstein-Friesian and Jersey bull calves in response to gradual weaning, and (ii) the effect of breed on immune response genes in bovine whole blood using real-time quantitative PCR. Holstein-Friesian and Jersey bull calves were group housed indoors and individually fed using an automatic feeder. They were allocated to a high, medium or low plane of nutrition, based on milk replacer (MR) and concentrate. The nutrition treatments were calculated using National Research Council guidelines in order to achieve a high, medium or low growth rate for each respective breed. During the weaning phase MR was gradually reduced over a 14-day (d) period (d −13 to d 0). Calves were blood sampled on d −14, −6, −3, 0, 1, 3, 8 and 14 relative to weaning (d 0) for subsequent haematological analysis. On d −14, 1 and 8, a subset of eight Holstein-Friesian calves randomly selected from the medium nutrition treatment and eight Jersey calves randomly selected from the high nutrition treatment, were blood sampled for gene expression profiling, targeting biomarkers of weaning stress. These two treatment groups were chosen to examine the effect of breed on expression of the genes of interest, as energy intake and animal performance were similar. There was no effect of breed × plane of nutrition interaction nor effect of plane of nutrition on any variable measured ( P > 0.05). Gradual weaning produced differential biological responses in the two breeds evidenced by breed × time interactions for lymphocyte, monocyte and red blood cell number, plasma haemoglobin and haptoglobin concentrations ( P < 0.05). The typical stress response consisting of neutrophilia and lymphopaenia was not observed for any treatment. An immune response to gradual weaning was observed as the relative gene expression level of the pro-apoptotic gene, Fas, increased on d 1 relative to d −14 ( P < 0.05). Relative gene expression levels were greater in Jersey calves compared with Holstein-Friesian for the pro-inflammatory cytokine CXCL8 ( P = 0.05) and the glucocorticoid receptor, GRα ( P < 0.05). The increased levels of these transcripts suggest that Jersey calves may have a more sensitive immune system compared with Holstein-Friesian.

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Section: Figurementioning

confidence: 45%

Characterisation of haematological profiles and whole blood relative gene expression levels in Holstein-Friesian and Jersey bull calves undergoing gradual weaning

Johnston

Kenny

Kelly

et al. 2016

animal

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“…The primary objective of this study was to investigate whether activation of this neuro-endocrine response was associated with the up-regulation of genes involved in the acute phase response. Although the mechanism of APP induction in response to stress is yet to be elucidated, activation of the hypothalamic-pituitary-interrenal axis in fish by stress may be the trigger of systemic or local (intra-pituitary) cytokine production, resulting in hepatic APP synthesis and subsequent release of the acute phase proteins into the bloodstream (Murata et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Acute phase gene expression in rainbow trout (Oncorhynchus mykiss) after exposure to a confinement stressor: A comparison of pooled and individual data

Talbot

Pottinger

Smith

et al. 2009

Fish & Shellfish Immunology

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“…APP are mainly of hepatic origin, and its synthesis is regulated by pro-inflammatory cytokines, mainly interleukin-6 (Baumann and Gauldie, 1994;Gabay and Kushner, 1999). Recent studies suggest that APP may be used to assess stress in farm animals (Murata et al, 2004;Piñ eiro et al, 2006). In animals for experimentation, physical and psychological stress elevate APP concentration (Morimoto et al, 1987;Deak et al, 1997) and increases of APP have been also observed in cattle after physical stress (Alsemgeest et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Pig acute-phase protein levels after stress induced by changes in the pattern of food administration

Piñeiro¹,

Piñeiro²,

Morales³

et al. 2007

Animal

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A total of 240 pigs, 74 days old, half boars and half females, were included in a trial designed to assess the effect of the stress caused by changes in the pattern of food administration on the concentration of acute phase proteins (APP) and productive performance parameters. Half of the animals (pigs fed ad libitum, AL group) had free access to feed, while the rest were fed following a disorderly pattern (DIS group), in which animals had alternating periods of free access to feed and periods of no feeding, when food was removed from the feeder. The periods of free access to feed (two daily periods of 2-h duration) were randomly assigned, and varied from day to day. Total feed supplied per day was identical in both groups, and exceeded the minimal amount required for animals of these ages. Pen feed intake, individual body weights and the main positive pig APP pig major acute phase protein (Pig-MAP), haptoglobin, serum amyloid A (SAA), C-reactive protein (CRP), and the negative APP apolipoprotein A-I (ApoA-I) and transtherytin were determined every 2 weeks during the period 76 to 116 days of age. Animals fed ad libitum had better average daily gain (ADG) than DIS animals in the whole experimental period ( P , 0.01) but the differences in ADG were only produced in the two first experimental sub-periods (60 to 74 and 74 to 116 days of age), suggesting that the stress diminished when the animals get used to the DIS feeding. Interestingly differences in ADG between DIS and AL pigs were due to males, whereas no differences were observed between females. The same differences observed for ADG were found for APP. DIS males had higher Pig-MAP concentration than AL males at 74 and 116 days of age, lower ApoA-I concentration at 74 days of age and higher haptoglobin and CRP concentration at 116 days of age ( P , 0.05). The results obtained in this trial show an inverse relationship between weight gain and APP levels, and suggest that APP may be biomarkers for the evaluation of distress and welfare in pigs.

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Current research on acute phase proteins in veterinary diagnosis: an overview

Cited by 900 publications

References 163 publications

Characterisation of haematological profiles and whole blood relative gene expression levels in Holstein-Friesian and Jersey bull calves undergoing gradual weaning

Characterisation of haematological profiles and whole blood relative gene expression levels in Holstein-Friesian and Jersey bull calves undergoing gradual weaning

Acute phase gene expression in rainbow trout (Oncorhynchus mykiss) after exposure to a confinement stressor: A comparison of pooled and individual data

Pig acute-phase protein levels after stress induced by changes in the pattern of food administration

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