Current evidence in high throughput ultrafiltration toward the purification of monoclonal antibodies (mAbs) and biotechnological protein-type molecules

Castro‐Muñoz, Roberto; Serna-Vázquez, Julio; García‐Depraect, Octavio

doi:10.1080/07388551.2021.1947182

Cited by 10 publications

(6 citation statements)

References 59 publications

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“…Protein ultrafiltration using MWCO membrane enables protein solution purification and increased sample concentration. It has been used to purify proteins, including antibodies and viruses [ 25 , 26 ]. However, it is not widely applied to medical purposes or in clinical practices.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique

et al. 2023

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Dengue infection has been a public health problem worldwide, especially in tropical areas. A lack of sensitive diagnostic methods in the early phase of the illness is one of the challenging problems in clinical practices. We, herein, analyzed 86 sera of acute febrile patients, from both dengue and non-dengue febrile illness, to study the diagnostic performance of dengue diagnostics. When compared with detection by Polymerase Chain Reaction (PCR), dengue NS1 detection by enzyme-linked immunosorbent assay (ELISA) had the highest sensitivity of 82.4% (with 94.3% specificity), while NS1 by rapid diagnostic test (RDT) had 76.5% sensitivity. IgM detection by ELISA and RDT showed only 27.5% and 17.9% sensitivity, respectively. The combination of NS1 and IgM in RDT yielded a sensitivity of 78.4%, with 97.1% specificity. One of the essential steps in making a diagnosis from patient samples is the preparation process. At present, a variety of techniques have been used to increase the number of analytes in clinical samples. In this study, we focused on the sample concentration method. The sera were concentrated three times with the ultrafiltration method using a 10 kDa molecular weight cut-off membrane. The results showed an increase in the sensitivity of RDT-NS1 detection at 80.4%, with 100% specificity. When combining NS1 and IgM detection, the concentration method granted RDT an 82.4% sensitivity, with 100% specificity. In conclusion, serum concentration by the ultrafiltration method is a simple and applicable technique. It could increase the diagnostic performance of point-of-care dengue diagnostics.

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Section: Discussionmentioning

confidence: 99%

Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique

et al. 2023

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“…In the following subsections, some of these methods, recognized for their high applicability potential, are discussed individually. A successful technical process should fulfill key criteria, including simplicity, rapidity, and non‐denaturation of the target proteins, enabling it to be scaled up for industrial applications (Castro‐Muñoz et al., 2022). Figure 2 illustrates the purification techniques of GMP in various processes.…”

Section: Extraction and Purification Methodsmentioning

confidence: 99%

Glycomacropeptide: A comprehensive understanding of its major biological characteristics and purification methodologies

Ebrahimi,

Andishmand,

Huo

et al. 2024

Comp Rev Food Sci Food Safe

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Glycomacropeptide (GMP) is a bioactive peptide derived from whey protein, consisting of 64 amino acids. It is a phenylalanine‐free peptide, making it a beneficial dietary option for individuals dealing with phenylketonuria (PKU). PKU is an inherited metabolic disorder characterized by high levels of phenylalanine in the bloodstream, resulting from a deficiency of phenylalanine dehydrogenase in affected individuals. Consequently, patients with PKU require lifelong adherence to a low‐phenylalanine diet, wherein a significant portion of their protein intake is typically sourced from a phenylalanine‐free amino acid formula. GMP has several nutritional values, numerous bioactivity properties, and therapeutic effects in various inflammatory disorders. Despite all these features, the purification of GMP is an imperative requirement; however, there are no unique methods for achieving this goal. Traditionally, several methods have been used for GMP purification, such as thermal or acid treatment, alcoholic precipitation, ultrafiltration (UF), gel filtration, and membrane separation techniques. However, these methods have poor specificity, and the presence of large amounts of impurities can interfere with the analysis of GMP. More efficient and highly specific GMP purification methods need to be developed. In this review, we have highlighted and summarized the current research progress on the major biological features and purification methodologies associated with GMP, as well as providing an extensive overview of the recent developments in using charged UF membranes for GMP purification and the influential factors.

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“…However, an escalation in the abundance of hydroxyl and carboxylic groups, alongside an acidic pH characteristic of the feed samples, engenders intermolecular negative polarity. Consequently, solutes engender water molecule attraction, resulting in increased volumetric dimensions of target molecules and impeding their permeation through membrane pores, thus engendering the "polarity resistance" phenomenon [35,36].…”

Section: Flavonoidsmentioning

confidence: 99%

“…Consequently, the MWCO serves as a relative rather than absolute barrier for the segregation of macro-and micro-molecules. Additionally, within bioresource matrices, larger and smaller functional molecules tend to aggregate into clusters; for instance, phenols may non-covalently bind to dietary fibers or proteins [35]. A reduction in the MWCO of the membrane is also evident during polarization concentration and fouling issues.…”

Section: Flavonoidsmentioning

confidence: 99%

Nanofiltration-Assisted Concentration Processes of Phenolic Fractions and Carotenoids from Natural Food Matrices

Castro-Muñoz

2024

Separations

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In new food formulations, carotenoids and phenolic compounds are likely to be the most sought after food ingredients according to their bioactivity, nutraceutical, nutritional value, and compatibility properties once incorporated into food formulations. Such solutes are naturally present in many plant-based sources, and some portions are directly consumed when enriching food products and formulations; however, some portions, which are contained in the parts of the plant sources not considered edible, including the leaves, peel, and seeds, among other by-products, are commonly wasted. Related to this, scientists have found a new window for obtaining these bioactive molecules, but their recovery remains a challenge. To some extent, the final purification and polishing requires highly selective performance to guarantee the desired properties and concentration. In this regard, membrane technologies, such as nanofiltration (NF), represent an alternative, owing to their highly selective properties when separating low-molecular-weight compounds. NF becomes immediately suitable when the pretreated extracts are subjected to further efficient concentration, fractionation, and polishing of phenolic fractions and carotenoids. The separation efficiency (usually higher than 97%) of NF technology is high according to the low pore size of NF membranes, but the low temperature in process separation also contributes to the separation of thermolabile compounds. Therefore, this paper reviews the ongoing cases of studies reporting the successful separation and polishing of phenolic fractions and carotenoids from distinct sources. In particular, we have focused our attention on the main interactions during the separation process and the drawbacks and advantages of using membranes for such a case study.

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Current evidence in high throughput ultrafiltration toward the purification of monoclonal antibodies (mAbs) and biotechnological protein-type molecules

Cited by 10 publications

References 59 publications

Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique

Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique

Glycomacropeptide: A comprehensive understanding of its major biological characteristics and purification methodologies

Nanofiltration-Assisted Concentration Processes of Phenolic Fractions and Carotenoids from Natural Food Matrices

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