“…When an embryo was detected it was washed and transferred to a small Petri dish containing modified Dulbecco's medium (Ovum Culture Medium; Cytosystems, Sydney, Australia). The flushing system was that described previously by Pashen (1985) using a 24 French gauge catheter (equine egg collection catheter; Rusch, High Wycombe, Bucks, UK) with the inlet port connected to the medium bag and the outlet port connected by Silastic tubing to an embryo filter (EmCon, Spring Valley, USA).…”