Current Approaches to Identification of Fusarium Fungi Infecting Wheat

Karelov, A. V.; Borzykh, O.; Козуб, Н. А.; Созинов, И. А.; Yanse, L.; Sozinova, O. I.; Tkalenko, H.; Mіshchеnkо, Lidiya; Blume, Ya. B.

doi:10.3103/s0095452721050030

Cited by 7 publications

(3 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unidentified isolates were confirmed to be trichothecene producers only using the Tri5F/R primer pair and were morphologically distinct from other B-type trichothecene-producing Fusarium species (F. culmorum, F. cerealis, F. poae, and F. equiseti). Our results are congruent with those of Župunski et al [10] and Karlov et al [31] who reported that FgrF/FgcR primers had higher specificity in distinguishing members of the F. graminearum clade from other causal agents of FHB in wheat. However, Župunski et al [10] did not test the applicability of Fg16F/R and FgrF/FgcR species-specific primer pairs neither on rye, triticale and oats nor on wheat grown in combination with pea as intercrops.…”

Section: Discussionsupporting

confidence: 93%

Effectiveness of Species- and Trichothecene-Specific Primers in Monitoring Fusarium graminearum Species Complex in Small Grain–Pea Intercropping Systems

Župunski

Jevtić

Grčak³

et al. 2022

Agriculture

View full text Add to dashboard Cite

Tracking the distribution of Fusarium species and the detection of changes in toxin production provides epidemiological information that is essential for Fusarium head blight (FHB) management. Members of Fusarium graminearum species complex (FGSC) were characterized using species and trichothecene-specific primers. Associations between members of the FGSC, cereal crop species (wheat, rye, triticale, and oat), seeding time (winter and spring), type of cultivation (monocrop and intercrop) and chemotype grouping were investigated with multiple correspondence analysis and multiple regression modeling. We found that triticale and oat were more related to isolates classified into F. graminearum s. lato than with other isolates. In contrast, wheat and rye were more associated with F. graminearum s. stricto. Cereal crop species affected the frequencies of F. graminearum s. stricto (p = 0.003) and F. graminearum s. lato (p = 0.08) and unidentified isolates with morphological characteristics like those of FGSC members (p = 0.02). The effectiveness of species-specific primers was 60.3% (Fg16F/R) and 76.2% (FgrF/FgcR), and the effectiveness of primer sets for the trichothecene genotyping of the Tri5 and Tri3 genes was 100% and 90.6%, respectively. The decrease in Fusarium-damaged kernel values in the wheat–pea intercropping system indicated that intercropping systems have the potential to control FHB.

show abstract

Section: Discussionsupporting

confidence: 93%

Effectiveness of Species- and Trichothecene-Specific Primers in Monitoring Fusarium graminearum Species Complex in Small Grain–Pea Intercropping Systems

Župunski

Jevtić

Grčak³

et al. 2022

Agriculture

View full text Add to dashboard Cite

show abstract

“…A variety of different methods are currently applied in the detection and identification of Fusarium species in cereals-for a recent review, see Karelov et al [14]. Traditional plating methods on a selective nutrient medium applied in the detection of Fusarium fungi are laborious and some species can outgrow others, preventing the detection of the whole Fusarium community.…”

Section: Introductionmentioning

confidence: 99%

“…The co-occurrence of several Fusarium species in cereals makes monitoring more challenging, and the detection of only a few Fusarium species is not sufficient. Genus-specific conventional PCR methods are available for the detection of the presence of Fusarium fungi in cereals [14], but a genus-specific quantitative PCR method for quantification of all the Fusarium species is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Taqman qPCR Quantification and Fusarium Community Analysis to Evaluate Toxigenic Fungi in Cereals

Sohlberg

Virkajärvi

Parikka

et al. 2022

Toxins

View full text Add to dashboard Cite

Fusarium head blight (FHB) is an economically important plant disease. Some Fusarium species produce mycotoxins that cause food safety concerns for both humans and animals. One especially important mycotoxin-producing fungus causing FHB is Fusarium graminearum. However, Fusarium species form a disease complex where different Fusarium species co-occur in the infected cereals. Effective management strategies for FHB are needed. Development of the management tools requires information about the diversity and abundance of the whole Fusarium community. Molecular quantification assays for detecting individual Fusarium species and subgroups exist, but a method for the detection and quantification of the whole Fusarium group is still lacking. In this study, a new TaqMan-based qPCR method (FusE) targeting the Fusarium-specific elongation factor region (EF1α) was developed for the detection and quantification of Fusarium spp. The FusE method was proven as a sensitive method with a detection limit of 1 pg of Fusarium DNA. Fusarium abundance results from oat samples correlated significantly with deoxynivalenol (DON) toxin content. In addition, the whole Fusarium community in Finnish oat samples was characterized with a new metabarcoding method. A shift from F. culmorum to F. graminearum in FHB-infected oats has been detected in Europe, and the results of this study confirm that. These new molecular methods can be applied in the assessment of the Fusarium community and mycotoxin risk in cereals. Knowledge gained from the Fusarium community analyses can be applied in developing and selecting effective management strategies for FHB.

show abstract

Native fungal community remains resilient during bioremediation of DBP pollution by exogenous Gordonia phthalatica QH-11T

Kong

Cernava

Wang

et al. 2023

Science of The Total Environment

View full text Add to dashboard Cite

Current Approaches to Identification of Fusarium Fungi Infecting Wheat

Cited by 7 publications

References 75 publications

Effectiveness of Species- and Trichothecene-Specific Primers in Monitoring Fusarium graminearum Species Complex in Small Grain–Pea Intercropping Systems

Effectiveness of Species- and Trichothecene-Specific Primers in Monitoring Fusarium graminearum Species Complex in Small Grain–Pea Intercropping Systems

Taqman qPCR Quantification and Fusarium Community Analysis to Evaluate Toxigenic Fungi in Cereals

Native fungal community remains resilient during bioremediation of DBP pollution by exogenous Gordonia phthalatica QH-11T

Contact Info

Product

Resources

About