Curcuminoid’s Content and Fingerprint Analysis for Authentication and Discrimination of Curcuma xanthorrhiza from Curcuma longa by High-Performance Liquid Chromatography-Diode Array Detector

Abstract

An accurate and reliable method for authentication and discrimination of Curcuma xanthorrhiza (CX) from Curcuma longa (CL) by determining the curcuminoid's content and analyzing the HPLC fingerprint combined with discriminant analysis (DA) was developed. By using the proposed method, it was found that CL had higher amount of all curcuminoid compounds compared to CX. Therefore, these two closely related species could be authenticated and discriminated by the amount of curcuminoids present in the samples. Authentication and discrimination of the two species were also achieved by comparing their HPLC fingerprint chromatograms using their typical marker peaks. To be more convincing, an aid from DA was also used. Combination of HPLC fingerprint analysis and DA gave excellent result that the two species were separated clearly, including CX samples adulterated with CL. The developed method was successfully used for quality control of the two plants.

Assessment of Genetic and Chemical Variability in Curcumae Longae Rhizoma (<i>Curcuma longa</i>) Based on DNA Barcoding Markers and HPLC Fingerprints

“…2,9,12,16,33,34) However, little is known about the relationship of genetic and chemical differences by using integrated approach. In this study, we found a low correlation (r=0.1721, p=0.047) between genetic distance and HPLC distance using the Mantel test.…”

Application of 1h-NMR Specta and Multivariate Analysis for the Authentication of Curcuma Xanthorrhiza From Zingiber Cassumunar

“…Because the adulteration practice of herbal plant used as raw material has became a serious problem, the authentication of C. xanthorrhiza needs to be performed in order to ensure its quality, safety, and efficacy [10,11]. The adulteration practice, either intentionally or unintentionally, usually includes partial or full substitution of original crude drugs with other substances which is cheaper, either free from or inferior in therapeutic properties.…”

Rapid Authentication of Turmeric Powder Adulterated With Curcuma Zedoaria and Curcuma Xanthorrhiza Using Ftir-Atr Spectroscopy and Chemometrics

“…xanthorrhiza [7]. For this reason, turmeric has been extensively used as a food coloring and as a raw ingredient of herbal supplements [1].…”

“…Therefore, the presence of other Curcuma species, such as C. zedoaria and C. xanthorhhiza, has been detected in turmeric powder sold in India [9]. Some analytical methods have been developed for authenticating medicinal plants, including fingerprint analysis using High-Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) combined with chemometrics [7], metabolomics technique using Proton Nuclear Magnetic Resonance (H-NMR) combined with multivariate analysis [10], and DNA based approach using DNA Barcoding [9], Random Amplified Polymorphic DNA (RAPD) [11], and Sequence Characterized Amplified Region (SCAR) [12]. The main disadvantage of these methods is that several steps are needed for sample preparation, making the process slow and costly.…”