Curcuminoid-loaded poly(methyl methacrylate) nanoparticles for cancer therapy

Abstract

Curcuminoids (Curs), oleoresins from Curcuma longa L., have known anticarcinogenic and anti-inflammatory properties, but high toxicity, poor aqueous solubility and susceptibility to degradation in body fluids are deterrents to their clinical administration. Poly(methyl methacrylate) nanoparticles (PMMA-NPs) are biocompatible and resilient and can entrap hydrophobic drugs. The present investigation is related to solubilizing Curs by incorporating them in these nanoparticles (NPs) and is related to a study comparing the anticarcinogenic effect of drug-loaded NPs with free Cur using lung cancer (A549) cell line. Freshly extracted oleoresins were post loaded in PMMA-NPs prepared using emulsion polymerization. The presence of the three components of oleoresins was confirmed by thin-layer chromatography. The size and morphology of void and loaded NPs were determined by dynamic light scattering, scanning electron microscopy and transmission electron microscopy. The NPs were spherical with diameters of 192.66±5 nm (void) and 199.16±5 nm (loaded). Drug loading and encapsulation efficiency were 6% and 93%, respectively. From the Fourier transform infrared spectroscopy spectra, the characteristic absorption vibration of poly(methyl methacrylate) and the bands at 1,383, 1,233 and 962 cm−1 for Cur moiety were observed. Drug release up to 10 days was estimated in buffer, saline and serum. The highest release of ~55% in ~3 days was noted in buffer that exhibited the highest bioavailability. The in vitro anticancer activity of loaded drug was evaluated up to 72 hours by MTT assay using A549 cell line. Cellular uptake of dye-loaded NPs was visualized within 30 minutes of incubation. The results revealed that the dose- and time-dependent cell death in case of loaded PMMA-NPs was comparable to that of free Cur. According to the study, the drug-loaded PMMA-NPs appear to be highly suitable for effective, localized and safe chemotherapy.

Targeted delivery of poly (methyl methacrylate) particles in colon cancer cells selectively attenuates cancer cell proliferation

“…PMMA particles have been shown to inhibit metastatic cancer cells [22]. Moreover, curcuminoid-loaded PMMA particles have been reported to inhibit the cancer cell proliferation [23]. In another report, PMMA conjugated with arginine, glycine, aspartic acid (RGD) peptide sequence showed strong anti-cancer activities [21].…”

“…PMMA is basically biocompatible polyester with high resistance to chemical hydrolysis, and high drug permeability and most important is that it does not cause toxicity [19]. Recently, a few studies have been demonstrated that different forms and structures of PMMA possess anti-cancer properties [20][21][22][23][24]. There is no report on the effect of PMMA (400-500 mm) on cancer cell viability and so considering various beneficial attributes of PMMA, we have decided to study the effect of PMMA on cancer cells.…”

Release Characteristics of Enoxaparin Sodium Loaded Polymethylmethacrylate Bone Cement

“…The pores between the beads are the carriers and space for drug release. The PMMA bone cement absorbs water through these pores and releases the water-soluble drugs by dispersion [10,11] . ES is a mucopolysaccharide substance with a relative molecular mass (Mr) of 3500-5500 with good water solubility.…”

In Vivo Antidepressant Effect of Passiflora edulis f. flavicarpa into Cationic Nanoparticles: Improving Bioactivity and Safety

“…The therapeutic use of natural compounds requires an efficient extraction process that is able to provide enough phytoactive compounds that are necessary to overcome the biological barriers and induce the desired pharmacological response [18,19]. Nano-delivery systems have shown interesting and promising properties for enhancing the activity of natural compounds [18,19]. These small particles could have the ability to overcome the blood-brain barrier and enhance drug targeting to the central nervous system (CNS) [20,21].…”