Curcumin suppresses inflammatory cytokines and heat shock protein 70 release and improves metabolic parameters during experimental sepsis

Abstract

Context: Curcumin has been reported to have anti-inflammatory, antioxidant and hypoglycaemic properties, besides reducing mortality in sepsis.Objective: This study evaluates the biological activities of a curcumin dispersion formulated by spray-drying in experimental sepsis.Materials and methods: Male Wistar rats were subjected to sepsis by caecal ligation and puncture (CLP), controls were sham operated. The animals were treated with curcumin dispersion (100 mg/kg, p.o.) or water for 7 days prior to CLP and at 2 h after surgery. One group was used to analyze curcumin absorption through HPLC; another had the survival rate assessed during 48 h; and from a third group, blood was collected by decapitation to analyze metabolic and inflammatory parameters.Results: The plasma curcumin levels reached 2.5 ng/mL at 4 h, dropped significantly (p < 0.001) at 6 h (1.2 ng/mL), and were undetectable at 24 h in both groups. Curcumin temporarily increased the survival rate of the septic rats by 20%. Moreover, it attenuated glycaemia (p < 0.05) and volemia (p < 0.05) alterations typically observed during sepsis, and decreased the levels of the proinflammatory cytokines IL-1β and IL-6 in plasma (p < 0.001) and peritoneal lavage fluid (p < 0.05) of septic rats. Serum HSP70 levels were decreased (p < 0.01) at 24 h after CLP.Discussion and conclusion: Our results show that the curcumin dispersion dose employed was not detrimental to the septic rats. In fact, it temporarily increased their survival rate, improved important metabolic parameters, reduced proinflammatory cytokines and HSP70 production.

Neuroinflammation and Cytokines in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS): A Critical Review of Research Methods

“…The bacteria used in dairy (i.e., the sour cream on the mashed potatoes) increase IL-1β, TNFα, and IFNγ (218, 219). Cumin, a spice commonly used in Indian cuisine, reduces expression of inflammatory cytokines CXL-1 and−2, TNFα, IL-1β, IL-6, and IL-18 (220, 221). Chocolate increases IL-10 and IL-1β (222).…”

Therapeutic effects of curcumin on sepsis and mechanisms of action: A systematic review of preclinical studies

“…In other study conducted by Silva et al (), 100 mg/kg/day curcumin in the form of oral gavage were reduced for 7 days by the proinflammatory cytokines and heat shock protein (HSP70) production and temporarily increased their survival rate.…”

“…In other study conducted by Silva et al (2017), 100 mg/kg/day curcumin in the form of oral gavage were reduced for 7 days by the proinflammatory cytokines and heat shock protein (HSP70) production and temporarily increased their survival rate. Madan and Ghosh (2003) demonstrated that oral curcumin supplementation for 7 days decreases the expression of ICAM-1 and vascular cell adhesion molecule 1 (VCAM-1) in liver and lungs and suppresses the migration and infiltration of neutrophils from blood vessels to the liver tissue.…”

Inflammatory cytokine expression in patients with sepsis at an intensive care unit

“…Although numerous strategies have been explored for the treatment of sepsis, affected patients still have an increased risk of mortality (6,7). A previous study also demonstrated that a reduction of inflammatory factors significantly improved metabolic parameters that are beneficial for survival in septic rats (8). In addition, distinct shock phenotypes were apparent, and the effect of early resuscitation according to protocols has been examined in selected cohorts of patients with septic shock (9).…”