Curcumin selectively induces colon cancer cell apoptosis and S cell cycle arrest by regulates Rb/E2F/p53 pathway

Li, Peihua; Pu, Shiming; Lin, Chao; Li, He; Zhao, Hongxia; Yang, Cheng; Guo, Ziqi; Xu, Shisan; Zhou, Zuping

doi:10.1016/j.molstruc.2022.133180

Cited by 23 publications

(24 citation statements)

References 41 publications

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“…The mechanism of action of curcumin was also partially characterized and shown to down-regulate several cell cycle proteins i.e . cyclin A2, cyclin E1, cell cycle dependent kinase 2 (CDK2), and transcription factor E2F1 ( Li et al, 2022 ). Altogether, Acanthamoeba autophagy and/or cell cycle pathway may be involved in our finding.…”

Section: Discussionmentioning

confidence: 99%

Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation

Boonhok

Sangkanu

Phumjan

et al. 2022

PeerJ

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Background Curcumin is an active compound derived from turmeric, Curcuma longa, and is known for its benefits to human health. The amoebicidal activity of curcumin against Acanthamoeba triangularis was recently discovered. However, a physiological change of intracellular pathways related to A. triangularis encystation mechanism, including autophagy in the surviving amoeba after curcumin treatment, has never been reported. This study aims to investigate the effect of curcumin on the survival of A. triangularis under nutrient starvation and nutrient-rich condition, as well as to evaluate the A. triangularis encystation and a physiological change of Acanthamoeba autophagy at the mRNA level. Methods In this study, A. triangularis amoebas were treated with a sublethal dose of curcumin under nutrient starvation and nutrient-rich condition and the surviving amoebas was investigated. Cysts formation and vacuolization were examined by microscopy and transcriptional expression of autophagy-related genes and other encystation-related genes were evaluated by real-time PCR. Results A. triangularis cysts were formed under nutrient starvation. However, in the presence of the autophagy inhibitor, 3-methyladenine (3-MA), the percentage of cysts was significantly reduced. Interestingly, in the presence of curcumin, most of the parasites remained in the trophozoite stage in both the starvation and nutrient-rich condition. In vacuolization analysis, the percentage of amoebas with enlarged vacuole was increased upon starvation. However, the percentage was significantly declined in the presence of curcumin and 3-MA. Molecular analysis of A. triangularis autophagy-related (ATG) genes showed that the mRNA expression of the ATG genes, ATG3, ATG8b, ATG12, ATG16, under the starvation with curcumin was at a basal level along the treatment. The results were similar to those of the curcumin-treated amoebas under a nutrient-rich condition, except AcATG16 which increased later. On the other hand, mRNA expression of encystation-related genes, cellulose synthase and serine proteinase, remained unchanged during the first 18 h, but significantly increased at 24 h post treatment. Conclusion Curcumin inhibits cyst formation in surviving trophozoites, which may result from its effect on mRNA expression of key Acanthamoeba ATG-related genes. However, further investigation into the mechanism of curcumin in A. triangularis trophozoites arrest and its association with autophagy or other encystation-related pathways is needed to support the future use of curcumin.

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Section: Discussionmentioning

confidence: 99%

Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation

Boonhok

Sangkanu

Phumjan

et al. 2022

PeerJ

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“…For example, the anticancer effect of curcumin has been indicated selectively induces colon cancer cell apoptosis and S cell cycle arrest by regulating Rb/E2F/p53 pathway. A selective effect has been indicated from the difference in the DNA repair system which is more efficient in normal cells than transformed cells [ 36 ]. The effect of isothiocyanates derived from Brassica plants comparing PC-3 prostate cancer cells and HDFa normal fibroblasts showing that the level of γ H2A.X, a marker of double-stranded DNA breaks, reduced more efficiently than in normal cells which indicated the activation of DNA repairing machinery [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Sonicated Extract from the Aril of Momordica Cochinchinensis Inhibits Cell Proliferation and Migration in Aggressive Prostate Cancer Cells

Chainumnim

Suksamrarn

Jarintanan

et al. 2022

Journal of Toxicology

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Momordica cochinchinensis or gac fruit has been reported to have several biological activities, including antioxidation, anti-inflammatory, and anticancer activities. However, the effect on cancer cell metastasis has not been extensively studied. With this aim, the extract from the aril part was selected and investigated for prostate cancer cell migration. The aril extracts were prepared as boiled extract, sonicated extract, ethanol extract, and HAE (hexane:acetone:ethanol; 2 : 1 : 1) extract, while the prostate cancer cell models were PC-3 and LNCaP cells. An MTT assay was performed to compare the antiproliferative effect between prostate cancer cells and normal Vero cells. As a result, the sonicated extract had the highest efficiency in PC-3 cells, with IC50 values of 2 mg/mL and 0.59 mg/mL for 48 and 72 h, respectively, while it had less of an effect in LNCaP cells and was not toxic to normal cells. Cell damage was further confirmed using LDH and cell cycle analysis. As a result, the sonicated extract did not cause cell damage or death and only inhibited cell proliferation. The effect on cancer metastasis was further examined by wound healing, transwell migration assays, and western blotting. The results demonstrated that the sonicated extract inhibited PC-3 cell migration and decreased MMP-9 but increased TIMP-1 expression. All these results support that gac fruit is a valuable source for further development as an anticancer agent for prostate cancer patients.

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“…In a recent paper, Obaidi and co-workers demonstrated that curcumin sensitized cancerous kidney cells to TRAIL-induced apoptosis via the Let-7C mediated deregulation of cell cycle proteins and cellular metabolism [ 168 ]. On the other hand, changes in mitochondria function, as well as the p53-dependent bax upregulation in breast [ 169 ] and colon cancer cells [ 36 ] and the bcl-2 down-regulation in laryngeal [ 170 ], breast [ 171 ] and prostate [ 172 ] cancer cells, are the most crucial changes that occur in curcumin-mediated intrinsic apoptosis. Furthermore, the ability of curcumin to activate caspase-8 in melanoma [ 173 ], and caspase-3 in hepatocellular carcinoma [ 167 ], neuroblastoma [ 174 ], linfoma [ 175 ] and lung cancer cells [ 176 ] confirmed its pro-apoptotic role.…”

Section: Polyphenol-mediated Apoptosis As Anticancer Mechanismmentioning

confidence: 99%

“…However, human epidemiological studies are not having much success in evaluating polyphenols’ effects due to their poor bioavailability and high metabolism rate [ 32 ]. Accumulating data showed in vitro polyphenols anti-proliferative effects on several cancer types, such as prostate [ 33 , 34 ], colon [ 35 , 36 ], breast [ 37 , 38 , 39 ], lung [ 40 , 41 ], bladder [ 42 , 43 ], pancreatic [ 44 , 45 ], leukemia [ 46 , 47 ], osteosarcoma [ 48 , 49 ] and ovarian [ 50 , 51 ], through different mechanisms of action. They can influence important molecular events related to carcinogenesis; these include the modulation of cell cycle signaling, induction of detoxifying and antioxidant enzyme systems, alteration of the epigenome and metabolism, and changes in the expression of key proteins involved in signal transduction pathways (e.g., MAPK and PI3K) or in the activity of transcription factors (e.g., NFkB) [ 52 , 53 , 54 ].…”

Section: Introductionmentioning

confidence: 99%

The Involvement of Natural Polyphenols in Molecular Mechanisms Inducing Apoptosis in Tumor Cells: A Promising Adjuvant in Cancer Therapy

Chimento

Luca

D’Amico

et al. 2023

IJMS

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Various literature data show how a diet rich in vegetables could reduce the incidence of several cancers due to the contribution of the natural polyphenols contained in them. Polyphenols are attributed multiple pharmacological actions such as anti-inflammatory, anti-oxidant, antibiotic, antiseptic, anti-allergic, cardioprotective and even anti-tumor properties. The multiple mechanisms involved in their anti-tumor action include signaling pathways modulation associated with cell proliferation, differentiation, migration, angiogenesis, metastasis and cell death. Since the dysregulation of death processes is involved in cancer etiopathology, the natural compounds able to kill cancer cells could be used as new anticancer agents. Apoptosis, a programmed form of cell death, is the most potent defense against cancer and the main mechanism used by both chemotherapy agents and polyphenols. The aim of this review is to provide an update of literature data on the apoptotic molecular mechanisms induced by some representative polyphenol family members in cancer cells. This aspect is particularly important because it may be useful in the design of new therapeutic strategies against cancer involving the polyphenols as adjuvants.

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Curcumin selectively induces colon cancer cell apoptosis and S cell cycle arrest by regulates Rb/E2F/p53 pathway

Cited by 23 publications

References 41 publications

Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation

Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation

Sonicated Extract from the Aril of Momordica Cochinchinensis Inhibits Cell Proliferation and Migration in Aggressive Prostate Cancer Cells

The Involvement of Natural Polyphenols in Molecular Mechanisms Inducing Apoptosis in Tumor Cells: A Promising Adjuvant in Cancer Therapy

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