Curcumin Protects against 1-Methyl-4-phenylpyridinium Ion- and Lipopolysaccharide-Induced Cytotoxicities in the Mouse Mesencephalic Astrocyte via Inhibiting the Cytochrome P450 2E1

Gui, Haiyan; Chen, Ruini; Peng, Yan; Hu, Jinhua; Zhao, Ming; Ning, Rui; Wang, Shang; Liu, Wei; Xiong, Jing; Hu, Gang

doi:10.1155/2013/523484

Cited by 9 publications

(6 citation statements)

References 44 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accordingly, any reagent which has the ability to either inhibit or downregulate CYP2E1 would be a strong candidate for the prevention and treatment of toxicity induced by ACR. 31,39 The present study showed that curcumin inhibited the ACRinduced CYP2E1 overexpression in the liver and kidney tissues, which was in line with previous observations that curcumin acted as a CYP2E1-positive inhibitor in the liver 20,40 and kidney. 41,42 In view of the essential role of CYP2E1 in the development of ACR hepatotoxicity and nephrotoxicity through the generation of free radicals and the reactive metabolites from metabolism of ACR, it could be speculated that curcumin might protect against ACR-induced hepatic and renal impairment through the downregulation of CYP2E1 expression.…”

supporting

confidence: 92%

Protective Effect of Curcumin on Acrylamide-Induced Hepatic and Renal Impairment in Rats: Involvement of CYP2E1

Rui

Chen

Cao

et al. 2020

Natural Product Communications

View full text Add to dashboard Cite

As a chemical extensively used in industrial areas and formed during heating of carbohydrate-rich foods and tobacco, acrylamide (ACR) has been demonstrated to exert a variety of systemic toxic effects including hepatotoxicity and nephrotoxicity. In the present study, we investigated the effect of curcumin, a natural polyphenolic compound in a popular spice known as turmeric, on the hepatic and renal impairment caused by ACR exposure to 40 mg/kg for 4 weeks in rats. The administration of curcumin at doses of 50 and 100 mg/kg to ACR-intoxicated rats significantly decreased the serum levels of alanine transaminase, aspartate transaminase, creatinine, and urea; improved the histological changes of liver and kidney caused by ACR; reduced the number of apoptotic cells; as well as relieved ACR-induced hepatic and renal oxidative stress. Moreover, curcumin inhibited the CYP2E1 overexpression induced by ACR in the liver and kidney tissues. Therefore, curcumin could be applied as a potential strategy for the intervention of ACR-induced systemic toxicity. The inhibition of CYP2E1 might be involved in the protection of curcumin against ACR-induced hepatotoxicity and nephrotoxicity.

show abstract

supporting

confidence: 92%

Protective Effect of Curcumin on Acrylamide-Induced Hepatic and Renal Impairment in Rats: Involvement of CYP2E1

Rui

Chen

Cao

et al. 2020

Natural Product Communications

View full text Add to dashboard Cite

show abstract

“…Also, CYP2E1, which is the hepatic P450 enzyme that contributes to the metabolic conversion of AOM into a colonic carcinogen, 56 has been observed to be inhibited by CUR. 57,58 Studies by Oetari et al suggest that, even at a CUR concentration of 30 μM, the extent of enzyme inhibition is <10%; therefore, we suspect that the effect of CUR was negligible. 57 Nevertheless, we cannot rule out the possibility that CUR and/or SAL supplementation confounded our results by altering AOM metabolism.…”

Section: Discussionmentioning

confidence: 88%

Curcumin and Salsalate Suppresses Colonic Inflammation and Procarcinogenic Signaling in High-Fat-Fed, Azoxymethane-Treated Mice

Wu¹,

Pfalzer

Koh³

et al. 2017

J. Agric. Food Chem.

View full text Add to dashboard Cite

High-fat diets (HFDs) and excess adiposity increase proinflammatory cytokines in the colon, altering gene expression in a manner that promotes the development of colorectal cancer (CRC). Thus, compounds that reduce this biochemical inflammation are potential chemopreventive agents. Curcumin (CUR), a dietary polyphenol, and salsalate (SAL), a non-steroidal anti-inflammatory drug, are both anti-inflammatories. We investigated the inhibitory effects of CUR with or without SAL on inflammatory cytokines and procarcinogenic signaling in azoxymethane (AOM)-treated A/J mice. A sub-tumorigenic AOM dose was chosen to produce a biochemical and molecular procarcinogenic colonic environment without tumors. Mice were fed either a HFD (60% of kilocalories) or low-fat diet (LFD) (10% of kilocalories). One HFD treatment group received 0.2% CUR in the diet; one received 0.2% CUR + 0.15% SAL; and one received 0.4% CUR + 0.3% SAL. The HFD mice developed 30% greater fat mass than the LFD mice (p < 0.05). The colonic concentrations of interleukin-1β (IL-1β) and interleukin-6 (IL-6) in the HFD mice were decreased by 50-69% by the high-dose combination regimen (p < 0.015). Only the combination regimens significantly suppressed phosphorylation of protein kinase B (Akt) and nuclear factor-κB (NF-κB) p65 (p < 0.044). The combination of CUR and SAL reduces the concentration of proinflammatory cytokines and diminishes activation of Akt and NF-κB more effectively than CUR alone, providing a scientific basis for examining whether this combination mitigates the risk of CRC in obese individuals.

show abstract

“…Likewise, a pure curcumin molecule showed effect on cancer prevention by inhibiting the activation of carcinogens or aflatoxin-DNA adduct by modulating the CYP450 activity [ 45 ]. Also in mouse brain astrocytes, pure curcumin inhibits CYP450 2E1 upon oxidative stress as its antioxidant properties [ 46 ]. Different Curcuma species from the same family Zingiberaceae have been studied.…”

Section: Discussionmentioning

confidence: 99%

Acute and Chronic Toxicity, Cytochrome P450 Enzyme Inhibition, and hERG Channel Blockade Studies with a Polyherbal, Ayurvedic Formulation for Inflammation

Dey¹,

Chaskar²,

Athavale³

et al. 2015

BioMed Research International

View full text Add to dashboard Cite

Ayurvedic plants are known for thousands of years to have anti-inflammatory and antiarthritic effect. We have recently shown that BV-9238, a proprietary formulation of Withania somnifera, Boswellia serrata, Zingiber officinale, and Curcuma longa, inhibits LPS-induced TNF-alpha and nitric oxide production from mouse macrophage and reduces inflammation in different animal models. To evaluate the safety parameters of BV-9238, we conducted a cytotoxicity study in RAW 264.7 cells (0.005–1 mg/mL) by MTT/formazan method, an acute single dose (2–10 g/kg bodyweight) toxicity study and a 180-day chronic study with 1 g and 2 g/kg bodyweight in Sprague Dawley rats. Some sedation, ptosis, and ataxia were observed for first 15–20 min in very high acute doses and hence not used for further chronic studies. At the end of 180 days, gross and histopathology, blood cell counts, liver and renal functions were all at normal levels. Further, a modest attempt was made to assess the effects of BV-9238 (0.5 µg/mL) on six major human cytochrome P450 enzymes and 3H radioligand binding assay with human hERG receptors. BV-9238 did not show any significant inhibition of these enzymes at the tested dose. All these suggest that BV-9238 has potential as a safe and well tolerated anti-inflammatory formulation for future use.

show abstract

Curcumin Protects against 1-Methyl-4-phenylpyridinium Ion- and Lipopolysaccharide-Induced Cytotoxicities in the Mouse Mesencephalic Astrocyte via Inhibiting the Cytochrome P450 2E1

Cited by 9 publications

References 44 publications

Protective Effect of Curcumin on Acrylamide-Induced Hepatic and Renal Impairment in Rats: Involvement of CYP2E1

Protective Effect of Curcumin on Acrylamide-Induced Hepatic and Renal Impairment in Rats: Involvement of CYP2E1

Curcumin and Salsalate Suppresses Colonic Inflammation and Procarcinogenic Signaling in High-Fat-Fed, Azoxymethane-Treated Mice

Acute and Chronic Toxicity, Cytochrome P450 Enzyme Inhibition, and hERG Channel Blockade Studies with a Polyherbal, Ayurvedic Formulation for Inflammation

Contact Info

Product

Resources

About