Curcumin Mediated Attenuation of Carbofuran Induced Oxidative Stress in Rat Brain

Abstract

The indiscriminate use of carbofuran to improve crop productivity causes adverse effects in nontargets including mammalian systems. The objective of this study was to evaluate carbofuran induced oxidative stress in rat brain stem and its attenuation by curcumin, a herbal product. Out of 6 groups of rats, 2 groups received two different doses of carbofuran, that is, 15 and 30% of LD50, respectively, for 30 days. Out of these, 2 groups receiving same doses of carbofuran were pretreated with curcumin (100 mg/kg body weight). The levels of antioxidants, TBARS, GSH, SOD, catalase, and GST were determined in rat brain stem. The 2 remaining groups served as placebo and curcumin treated, respectively. The data suggested that carbofuran at different doses caused significant alterations in the levels of TBARS and GSH in dose dependent manner. The TBARS and GSH contents were elevated. The activities of SOD, catalase, and GST were significantly inhibited at both doses of carbofuran. The ratio of P/A was also found to be sharply increased. The pretreatment of curcumin exhibited significant protection from carbofuran induced toxicity. The results suggested that carbofuran at sublethal doses was able to induce oxidative stress in rat brain which could be attenuated by curcumin.

Protective mechanism of turmeric (Curcuma longa) on carbofuran-induced hematological and hepatic toxicities in a rat model

“…Similar observations were recorded in the rat's kidney when the animals were exposed to CF (1 mg/kg) for consecutively 28 days (Kaur et al 2012) and also in the rat's brain stem when the animals were exposed to CF at two different doses, i.e. 15% (1.2 mg/kg) and 30% (2.4 mg/kg) of LD 50 for 30 days (Jaiswal et al 2016). Co-administration with turmeric, however, improved the activities of SOD and GSH-Px by scavenging superoxide and hydrogen peroxide produced by CF (Jaiswal et al 2015(Jaiswal et al , 2016.…”

“…15% (1.2 mg/kg) and 30% (2.4 mg/kg) of LD 50 for 30 days (Jaiswal et al 2016). Co-administration with turmeric, however, improved the activities of SOD and GSH-Px by scavenging superoxide and hydrogen peroxide produced by CF (Jaiswal et al 2015(Jaiswal et al , 2016. Curcumin, the main active ingredient of turmeric is potentially responsible in boosting these enzyme activities as confirmed by Jaiswal et al (2016).…”

“…Co-administration with turmeric, however, improved the activities of SOD and GSH-Px by scavenging superoxide and hydrogen peroxide produced by CF (Jaiswal et al 2015(Jaiswal et al , 2016. Curcumin, the main active ingredient of turmeric is potentially responsible in boosting these enzyme activities as confirmed by Jaiswal et al (2016). The enzyme levels were also higher in animals administrated with turmeric alone when compared with the control group thus clearly indicating that turmeric does not only neutralize the oxidative stress but also boosts the activity of the antioxidant enzymes in normal physiological conditions.…”

Fermented Chinese Formula Shuan-Tong-Ling Protects Brain Microvascular Endothelial Cells against Oxidative Stress Injury

“…But the pretreatment of animals with curcumin showed attenuation of carbofuran toxicity because the activities of SOD and GSH of rat brain were significantly increased. The ameliorative effect of curcumin may be mediated via antioxidant potential of this molecule by scavenging the free radicals [17]. Moreover, curcumin treatment at both low and high doses significantly reduced levels of ROS and MDA and enhanced the activity of SOD and GSH-PX compared with the oxyhemoglobin group, and treatment with curcumin at both low and high doses significantly reduced the levels of TNF- α , IL-1 β , and IL-6 compared with the oxyhemoglobin group, suggesting that curcumin is protective against oxyhemoglobin induced neurotoxicity in an in vitro subarachnoid hemorrhage model and the protective effects of curcumin were mainly dependent on its attenuating oxidative stress, inflammation, and neuronal apoptosis [49].…”

“…Recent studies have shown that traditional Chinese herbs or formulae, such as puerarin [16], curcumin [17], and Buyang Huanwu Decoction [18], were popularly used to attenuate oxidative stress injury and protect endothelial cells from H 2 O 2 -induced apoptosis by inhibiting oxidative stress damage or ROS-mediated mitochondrial dysfunction. In traditional Chinese medicine, qi is an energy that can invigorate the body and enhance blood circulation and meridian circulation [19–21].…”