Curcumin may serve an anticancer role in human osteosarcoma cell line U-2 OS by targeting ITPR1

Luo, Zhanpeng; Li, Dawei; Luo, Xiaobo; Li, Litao; Gu, Shanqiang; Liu, Yu; Ma, Yan

doi:10.3892/ol.2018.8032

Cited by 11 publications

(13 citation statements)

References 46 publications

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“…In gastric cancer, curcumin and formononetin posed different mechanisms to enhance Dox cytotoxicity [ 59 , 60 ]. These natural compounds exhibit potentiation for main applications in cancer treatment, and also play a supporting role in Dox regimen to overcome the limitation of Dox usage [ 61 , 62 , 63 , 64 , 65 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

PG-Priming Enhances Doxorubicin Influx to Trigger Necrotic and Autophagic Cell Death in Oral Squamous Cell Carcinoma

Lin

Weng

2018

JCM

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Synergistic effects between natural compounds and chemotherapy drugs are believed to have fewer side effects with equivalent efficacy. However, the synergistic potential of prodigiosin (PG) with doxorubicin (Dox) chemotherapy is still unknown. This study explores the synergistic mechanism of PG and Dox against oral squamous cell carcinoma (OSCC) cells. Three OSCC cell lines were treated with different PG/Dox combinatory schemes for cytotoxicity tests and were further investigated for cell death characteristics by cell cycle flow cytometry and autophagy/apoptosis marker labelling. When OSCC cells were pretreated with PG, the cytotoxicity of the subsequent Dox-treatment was 30% higher than Dox alone. The cytotoxic efficacy of PG-pretreated was found better than those of PG plus Dox co-treatment and Dox-pretreatment. Increase of Sub-G1 phase and caspase-3/LC-3 levels without poly (ADP-ribose) polymeras (PARP) elevation indicated both autophagy and necrosis occurred in OSCC cells. Dox flux after PG-priming was further evaluated by rhodamine-123 accumulation and Dox transporters analysis to elucidate the PG-priming effect. PG-priming autophagy enhanced Dox accumulation according to the increase of rhodamine-123 accumulation without the alterations of Dox transporters. Additionally, the cause of PG-triggered autophagy was determined by co-treatment with endoplasmic reticulum (ER) stress or AMP-activated protein kinase (AMPK) inhibitor. PG-induced autophagy was not related to nutrient deprivation and ER stress was proved by co-treatment with specific inhibitor. Taken together, PG-priming autophagy could sensitize OSCC cells by promoting Dox influx without regulation of Dox transporter. The PG-priming might be a promising adjuvant approach for the chemotherapy of OSCC.

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Section: Discussionmentioning

confidence: 99%

PG-Priming Enhances Doxorubicin Influx to Trigger Necrotic and Autophagic Cell Death in Oral Squamous Cell Carcinoma

Lin

Weng

2018

JCM

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“…For osteosarcoma, several researchers have successfully proven that curcumin can induce the MG63, U2OS, and HOS cell line apoptosis based on different signal pathways (32,33,(47)(48)(49)(50). Besides, curcumin has also been identified to suppress the proliferation, invasion, and metastasis of osteosarcoma (23,24,51,52).…”

Section: Inhibition Effect Of Curcumin On Tumorsmentioning

confidence: 99%

Curcumin in Osteosarcoma Therapy: Combining With Immunotherapy, Chemotherapeutics, Bone Tissue Engineering Materials and Potential Synergism With Photodynamic Therapy

Wang

Guo

et al. 2021

Front. Oncol.

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Osteosarcoma is a dominating malignant bone tumor with high mortality due to pulmonary metastases. Furthermore, because of the cancer cell erosion and surgery resection, osteosarcoma always causes bone defects, which means dysfunction and disfigurement are seldom inevitable. Although various advanced treatments (e.g. chemotherapy, immunotherapy, radiotherapy) are coming up, the 5-year survival rate for osteosarcoma with metastases is still dismal. In line with this, the more potent treatments for osteosarcoma are in high demand. Curcumin, a perennial herb, has been reportedly applied in the therapy of various types of tumors via different mechanisms. In vitro, it has also been reported that curcumin can inhibit the proliferation of osteosarcoma cell lines and can be used to repair bone defects. This seems curcumin is a promising candidate in osteosarcoma treatment. However, due to its congenital property like hydrophobicity, and low bioavailability, affecting its anticancer effect, clinical applications of curcumin are highly limited. To enhance its performance in cancer therapies, some synergist approaches with curcumin have emerged. The present review presents some prospective ones (i.e. combinations with immunotherapy, chemotherapeutics, bone tissue engineering, and biomaterials) applied in osteosarcoma treatment. Additionally, with the advancements of photodynamic therapy in cancer therapy, this review also prospects the combination of curcumin with photodynamic therapy in osteosarcoma treatment.

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“…Overexpression of the EEF1A1 gene can specifically inhibit the TP53 gene [12], while TP53 is a well-known tumor suppressor gene. A study in 2018 proved that the EEF1A1 gene is involved in the occurrence of osteosarcoma through biological processes by constructing a PPI network and by GO analysis [13]. Figure 5B-5D shows that the EEF1A1 and YWHAZ genes were important genes in the network diagram of target genes.…”

Section: Discussionmentioning

confidence: 94%

“…Previous studies on whole-exome sequencing demonstrated that the TP53 and RB1 genes played a major role in recurrent changes in osteosarcoma (80-90% and 10-39%, respectively) [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. About 50% of patients with osteosarcoma have inactivating mutations in both the TP53 and RB1 genes.…”

Section: Discussionmentioning

confidence: 99%

Experimental Study of Somatic Variants of Osteosarcoma by Whole-Exome Sequencing

et al. 2020

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Background:This study aimed to investigate the role of gene mutation site distribution, biological function, pathway enrichment, and gene association analysis in the occurrence, development, and migration of osteosarcoma. Material/Methods:Somatic mutation screening was performed using the whole-exome sequencing of osteosarcoma samples, and the distribution of mutations was demonstrated by Circos diagrams. Metascape was used to analyze the GO and KEGG signal pathway enrichment of the genes harboring protein coding alterations, and GeneMANIA was used to analyze the interaction of mutated genes. Results:The results showed that the protein coding alterations were found throughout the whole genome in 3 osteosarcoma samples. A large number of identical or related biological processes and pathways were found in osteosarcoma samples. The GeneMANIA analysis of the 10 mutations shared by 3 samples showed that the target gene minichromosome maintenance complex component 4 (MCM4) and 3 lateral genes were most functional, and were all related to DNA replication. The analysis of GO and KEGG signal pathway enrichment showed that the mutated genes were involved mainly in tumor-related metabolic pathways. Three mutated genes were involved in the cell process, and 2 mutated genes were involved in the metabolic process. Known driver gene mutations were also observed in the samples. Conclusions:The gene analysis confirmed that patients with osteosarcoma had a wide range of common gene mutations related to each other, which are involved in tumor-related metabolic pathways. These findings provide a basis for further gene-targeted therapy and pathway research.

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Curcumin may serve an anticancer role in human osteosarcoma cell line U-2 OS by targeting ITPR1

Cited by 11 publications

References 46 publications

PG-Priming Enhances Doxorubicin Influx to Trigger Necrotic and Autophagic Cell Death in Oral Squamous Cell Carcinoma

PG-Priming Enhances Doxorubicin Influx to Trigger Necrotic and Autophagic Cell Death in Oral Squamous Cell Carcinoma

Curcumin in Osteosarcoma Therapy: Combining With Immunotherapy, Chemotherapeutics, Bone Tissue Engineering Materials and Potential Synergism With Photodynamic Therapy

Experimental Study of Somatic Variants of Osteosarcoma by Whole-Exome Sequencing

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