Curcumin Inhibits Papillary Thyroid Cancer Cell Proliferation by Regulating lncRNA LINC00691

Abstract

Papillary thyroid cancer (PTC) is a type of epithelial-derived differentiated TC that reportedly accounts for a majority of TCs. Curcumin, a polyphenolic compound and a member of the Zingiberaceae (ginger) family derived from turmeric plants, can exhibit anticancer effects. Herein, we aimed to investigate the effect of curcumin on PTC and elucidate underlying mechanisms. Accordingly, PTC B-CPAP cells were treated with curcumin, in combination with/without long noncoding RNA LINC00691 inhibition, to determine the effect of curcumin and its relationship with LINC00691 in PTC cells. We observed that curcumin treatment decreased B-CPAP cell proliferation and promoted apoptosis. Curcumin inhibited LINC00691 expression in B-CPAP cells. Curcumin administration or si-LINC00691 transfection alone promoted ATP levels, inhibited glucose uptake and lactic acid levels, and inhibited lactate dehydrogenase A and hexokinase 2 protein expression in B-CPAP cells, which were further enhanced by combination treatment. Moreover, curcumin administration or si-LINC00691 transfection alone inhibited p-Akt activity, further suppressed by combination treatment. Akt inhibition promoted apoptosis and suppressed the Warburg effect in B-CPAP cells. In conclusion, our findings indicate that curcumin promotes apoptosis and suppresses proliferation and the Warburg effect by inhibiting LINC00691 in B-CPAP cells. The precise molecular mechanism might be mediated through the Akt signaling pathway, providing a theoretical basis for the treatment of PTC with curcumin.

Curcuminoids as Modulators of EMT in Invasive Cancers: A Review of Molecular Targets With the Contribution of Malignant Mesothelioma Studies

“…Among new findings, curcumin's potential role in the treatment of resistant acute myeloid leukemia was shown to involve inhibition of the HOTAIR/miR-20a-5p/WT1 axis (Liu J. M. et al, 2021). Moreover, the inhibition of Warburg effect produced by curcumin in invasive thyroid cancer cells, linked to a reduction of the expression of another lncRNA, LINC00691, appeared to involve the Akt signaling pathway (Li Z. et al, 2022). Hao et al (2020) revealed that a new curcumin analog Comp34 decreased the expression of a newly identified oncogenic lncRNA, NUDT3-AS4, in triple-negative breast cancer cells, leading to the dissociation of the complex formed with miR-99s and finally to the degradation of AKT1/mTOR mRNA.…”

Contribution of Non-Coding RNAs to Anticancer Effects of Dietary Polyphenols: Chlorogenic Acid, Curcumin, Epigallocatechin-3-Gallate, Genistein, Quercetin and Resveratrol

“…In papillary thyroid cancer B-CPAP cells, CUR decreased cell proliferation, promoted apoptosis, and inhibited LINC00691 expression [ 163 ]. CUR administration or transfection of si-LINC00691 caused downregulation of AKT leading to apoptosis in these cells, suggesting that inhibition of LINC00691 is involved in the anticancer effect of CUR.…”

Research Trend and Detailed Insights into the Molecular Mechanisms of Food Bioactive Compounds against Cancer: A Comprehensive Review with Special Emphasis on Probiotics

“…Curcumin suppressed the Akt/PI3K/mTOR signaling axis and upregulated miR-199a to inhibit oral squamous cell carcinoma [ 85 ]. Curcumin suppressed papillary thyroid cancer by modulating the long non-coding RNA LINC00691 through the Akt signaling axis [ 86 ]. Curcumin also works on several other cancers such as glioblastoma by acting through MMP, NF-κB, STAT3, and PI3K/Ak/mTOR downregulation [ 87 ]; breast cancer by p53 regulation [ 88 ], and lung cancer metastasis inhibition by regulating the adiponectin/NF-κB/MMPs signaling pathway [ 89 ].…”