Curcumin I protects the dopaminergic cell line SH-SY5Y from 6-hydroxydopamine-induced neurotoxicity through attenuation of p53-mediated apoptosis

Jaisin, Yamaratee; Thampithak, Anusorn; Meesarapee, Benjawan; Ratanachamnong, Piyanee; Suksamrarn, Apichart; Phivthong-ngam, Laddawal; Morales, Noppawan Phumala; Chongthammakun, Sukumal; Govitrapong, Piyarat; Sanvarinda, Yupin

doi:10.1016/j.neulet.2010.12.014

Cited by 70 publications

(47 citation statements)

References 24 publications

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“…Curcumin has been recognized as an effective agent for neurodegenerative diseases, because of its neuroprotective effects in PD, AD, and ALS [45]. Curcumin also ameliorates neurotoxicity induced by various toxicants such as metals (fluoride, lead, and arsenic), solvents, 6-OHDA, and Aβ [36,37,[40][41][42][43][44]. Recently, we found that curcumin enhanced the adult hippocampal neurogenesis as well as improved learning and memory deficits in AD model via recruitment of the Wnt/β-catenin pathway [41].…”

Section: Discussionmentioning

confidence: 99%

“…Curcumin attenuates neurotoxicity induced by various toxicants such as fluoride, acrolein, okadaic acid, lead, ethanol, arsenic, sodium metabisulfite, 6-hydroxydopamine (6-OHDA), and amyloid-β (Aβ) [34][35][36][37][38][39][40][41][42][43][44]. Curcumin also provides neuroprotection in cellular and animal models of neurological and neurodegenerative disorders including Parkinson's disease (PD), Alzheimer's disease (AD), Huntington's disease (HD), depression, multiple sclerosis, and schizophrenia [45][46][47][48].…”

Section: Introductionmentioning

confidence: 99%

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Bisphenol-A Mediated Inhibition of Hippocampal Neurogenesis Attenuated by Curcumin via Canonical Wnt Pathway

et al. 2015

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Bisphenol A (BPA) is an environmental xenoestrogenic endocrine disruptor, utilized for production of consumer products, and exerts adverse effects on the developing nervous system. Recently, we found that BPA impairs the finely tuned dynamic processes of neurogenesis (generation of new neurons) in the hippocampus of the developing rat brain. Curcumin is a natural polyphenolic compound, which provides neuroprotection against various environmental neurotoxicants and in the cellular and animal models of neurodegenerative disorders. Here, we have assessed the neuroprotective efficacy of curcumin against BPA-mediated reduced neurogenesis and the underlying cellular and molecular mechanism(s). Both in vitro and in vivo studies showed that curcumin protects against BPA-induced hippocampal neurotoxicity. Curcumin protects against BPA-mediated reduced neural stem cells (NSC) proliferation and neuronal differentiation and enhanced neurodegeneration. Curcumin also enhances the expression/levels of neurogenic and the Wnt pathway genes/proteins, which were reduced due to BPA exposure in the hippocampus. Curcumin-mediated neuroprotection against BPA-induced neurotoxicity involved activation of the Wnt/β-catenin signaling pathway, which was confirmed by the use of Wnt specific activators (LiCl and GSK-3β siRNA) and inhibitor (Dkk-1). BPA-mediated increased β-catenin phosphorylation, decreased GSK-3β levels, and β-catenin nuclear translocation were significantly reversed by curcumin, leading to enhanced neurogenesis. Curcumin-induced protective effects on neurogenesis were blocked by Dkk-1 in NSC culture treated with BPA. Curcumin-mediated enhanced neurogenesis was correlated well with improved learning and memory in BPA-treated rats. Overall, our results conclude that curcumin provides neuroprotection against BPA-mediated impaired neurogenesis via activation of the Wnt/β-catenin signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bisphenol-A Mediated Inhibition of Hippocampal Neurogenesis Attenuated by Curcumin via Canonical Wnt Pathway

et al. 2015

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“…Bcl-2 is one of antiapoptotic Bcl-2 family proteins, which regulates the mitochondrial pathway [33]. Several studies showed that curcumin can block the oxidative stress mediated apoptosis via suppressing the mitochondria apoptotic pathway against cisplatin, palmitate and 6-hydroxydopamin induced cytotoxicity [47][48][49]. These data indicated that curcumin protected L02 cells against FZD induced apoptosis via inhibiting mitochondrial pathway.…”

Section: Discussionmentioning

confidence: 96%

“…Our previous studies had showed that p38 MAPK and GADD45a, a downstream gene of p53, participated in FZD induced cell cycle arrest and apoptosis [51,52]. Another study demonstrated that curcumin protected against 6-hydroxydopamine-induced neurotoxicity through attenuation of p53-mediated apoptosis in the dopaminergic cell line SH-SY5Y [47]. Consistently, the present study showed curcumin treatment markedly decreased the p53 gene expression caused by FZD (Figure 7), indicating that p53 pathway particulates in the protection role of curcumin.…”

Section: Discussionmentioning

confidence: 99%

Curcumin Ameliorates Furazolidone Induced DNA Damage and Apoptosis in Human Hepatocyte L02 Cells via Inhibiting ROS Production and Mitochondrial Pathway

Dai

Gong

et al. 2016

Preprint

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Furazolidone (FZD), a synthetic nitrofuran derivative, has been widely used as an antibacterial and antiprotozoal agent. Recently, the potential toxicity of FZD has raised concerns, but its mechanism is still unclear. This study aimed to investigate the protective effect of curcumin on FZD induced cytotoxicity and the underlying mechanism in human hepatocyte L02 cells. The results showed that curcumin pre-treatment significantly ameliorated FZD induced oxidative stress, characterized by decreased reactive oxygen species (ROS) and malondialdehyde formations, and increased superoxide dismutase, catalase activities and glutathione contents. In addition, curcumin pre-treatment significantly ameliorated the loss of mitochondrial membrane potential, the activation of caspase-9 and -3, and apoptosis caused by FZD. Alkaline comet assay showed that curcumin markedly reduced FZD-induced DNA damage in a dose-dependent manner. Consistently, curcumin pre-treatment markedly down-regulated the mRNA expression levels of p53, Bax, caspase-9 and -3 and up-regulated the mRNA expression level of Bcl-2. Taken together, these results revealed that curcumin protects against FZD induced DNA damage and apoptosis via inhibiting oxidative stress and mitochondrial pathway. Our study indicated that curcumin may be a promising combiner with FZD to reduce FZD-related toxicity in the clinical applications.

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“…31 Curcumin is a naturally derived polyphenol, obtained from a perennial herb, which has various therapeutic features including anti-inammatory, antitumor, antioxidant and antimicrobial activities, 32,33 and specifically, a neuroprotective activity. 34 The main disadvantages in using curcumin are its low solubility in water under an acidic media and its rapid hydrolysis under alkaline conditions.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of nanostructured alumina with ultrahigh pore volume for pH-dependent release of curcumin

2017

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In a new approach, a series of nanoporous non-crystalline alumina (NPA) materials was designed and developed by an evaporation-induced self-assembly method in the presence of various weight ) and large pore sizes (up to 23.0 nm). The results indicated that higher pore volume (2.00 cm 3 g À1 ) was obtained when only tetrabutylammonium bromide (namely NPA-100) was used than when only P123 namely NPA-0 (0.5 cm 3 g À1) was used as a template. These new supports were used, for the first time, as an inorganic host for poorly soluble guest of curcumin (CUR). XRD analysis confirmed the amorphous state of CUR after being loaded into the NPAs, which is indicative of the full dispersion of the drug into the pores. The in vitro release behavior of these compounds was studied in simulated gastric fluid (SGF, pH ¼ 1.2) and simulated intestinal fluid (SIF, pH ¼ 6.8). The result indicated that the release of insoluble curcumin reached 80% in SGF when it was supported on alumina with ultrahigh pore volume. Moreover, this carrier showed high drug adsorption. The potential of the NPA-100@CUR in protecting SH-SY5Y cells from oxidative stress by toxin 6-OHDA that caused parkinsonian was tested. The results indicated that the formulation neutralizes 6-OHDA toxicity and significantly enhances cell survival.

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Curcumin I protects the dopaminergic cell line SH-SY5Y from 6-hydroxydopamine-induced neurotoxicity through attenuation of p53-mediated apoptosis

Cited by 70 publications

References 24 publications

Bisphenol-A Mediated Inhibition of Hippocampal Neurogenesis Attenuated by Curcumin via Canonical Wnt Pathway

Bisphenol-A Mediated Inhibition of Hippocampal Neurogenesis Attenuated by Curcumin via Canonical Wnt Pathway

Curcumin Ameliorates Furazolidone Induced DNA Damage and Apoptosis in Human Hepatocyte L02 Cells via Inhibiting ROS Production and Mitochondrial Pathway

Synthesis of nanostructured alumina with ultrahigh pore volume for pH-dependent release of curcumin

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