Curcumin‐embedded DBPC liposomes coated with chitosan layer as a fluorescence nanosensor for the selective detection of ribonucleic acid

Abstract

One of the limitations of fluorescence probe molecules during biomedical estimation is their lack of ability to selectively determine the targeted species. To overcome this there have been various approaches that involve attaching a functional group or aptamers to the fluorescence probe. However, encapsulating probe molecules in a matrix using nanotechnology can be a viable and easier method. Curcumin (Cur) as a fluorescence marker cannot distinguish DNA and RNA. This research reports a novel selective approach involving the use of nanocapsules composed of liposomal curcumin coated with chitosan for the selective detection of RNA molecules using a fluorescence method. The increase in RNA concentration enhanced the electrostatic interaction between the negatively charge surface of RNA and the positively charged nanocapsule, which was further verified by zeta potential measurement. This method had a low limit of detection (36 ng/ml) and higher linear dynamic ranges compared with other studies found in the literature. Moreover, the method was not affected by DNA and was selective for the detection of RNA molecules for which the site of interaction was confined only to uracil. The selectivity for RNA molecules towards other analogues species was also examined and recovery range found was between 99 and 100.33%.

Liposome-based nanocapsules for the controlled release of dietary curcumin: PDDA and silica nanoparticle-coated DMPC liposomes enhance the fluorescence efficiency and anticancer activity of curcumin

“…Therefore, improving the bioavailability of dietary curcumin is of great importance. Recently, curcumin has been encapsulated in different matrices, such as DBPC liposomes, 25 DAPC liposomes, 26 and F108 polymer. 27 In this work, we have prepared different types of liposome-mediated PDDA nanocapsules using DMPC and tested the release of curcumin at different pH, assessed fluorescence spectral change, and investigated their anticancer activity, which have shown remarkably promising results.…”

Wearable Natural Rubber Latex Gloves with Curcumin for Torn Glove Detection in Clinical Settings

“…Curcumin-based fluorescent probes can solve the lack of organic fluorescent dyes, for example, low quantum yield, and poor photostability [ 13 , 14 ]. Furthermore, since curcumin possesses multifunctional based fluorescence probes with high sensitivity, selectivity, and stability, much research has been published on chemosensor [ 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ] and biosensor [ 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 ] applications of curcumin [ 12 ]. Thus, a strong body of foundational knowledge relating to the use of this compound is readily available.…”