Curcumin ameliorates H&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;-induced injury through SIRT1-PERK-CHOP pathway in pancreatic beta cells

Cao, Zhijian; Wu, Zhuan; Duan, Tingting; Tang, Cifei; Huang, Di

doi:10.3724/abbs.2022004

Cited by 9 publications

(6 citation statements)

References 31 publications

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“…A total of 2 × 10 5 cells per well were inoculated in 6-well plates, and each group of cells was treated following the method described by Cao et al After that, the samples to be stained were fully covered with the appropriate amount of the Hoechst 33258 staining solution. The cells were then incubated at 37 °C and 5% CO 2 for 30 min, and the staining solution was discarded, and the cells were washed three times with PBS before observing them under a fluorescence microscope (Nikon, Eclipse Cl, Japan).…”

Section: Methodsmentioning

confidence: 99%

Morin-Based Nanoparticles for Regulation of Blood Glucose

Hua,

Li,

Chen

et al. 2024

ACS Appl. Mater. Interfaces

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Morin, a naturally occurring bioactive compound shows great potential as an antioxidant, anti-inflammatory agent, and regulator of blood glucose levels. However, its low water solubility, poor lipid solubility, limited bioavailability, and rapid clearance in vivo hinder its application in blood glucose regulation. To address these limitations, we report an enzymatically synthesized nanosized morin particle (MNs) encapsulated in sodium alginate microgels (M@SA). This approach significantly enhances morin's delivery efficiency and therapeutic efficacy in blood glucose regulation. Utilizing horseradish peroxidase, we synthesized MNs averaging 305.7 ± 88.7 nm in size. These MNs were then encapsulated via electrohydrodynamic microdroplet spraying to form M@SA microgels. In vivo studies revealed that M@SA microgels demonstrated prolonged intestinal retention and superior efficacy compared with unmodified morin and MNs alone. Moreover, MNs notably improved glucose uptake in HepG2 cells. Furthermore, M@SA microgels effectively regulated blood glucose, lipid profiles, and oxidative stress in diabetic mice while mitigating liver, kidney, and pancreatic damage and enhancing antiinflammatory responses. Our findings propose a promising strategy for the oral administration of natural compounds for blood glucose regulation, with implications for broader therapeutic applications.

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Section: Methodsmentioning

confidence: 99%

Morin-Based Nanoparticles for Regulation of Blood Glucose

Hua,

Li,

Chen

et al. 2024

ACS Appl. Mater. Interfaces

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“…Changes in the cell apoptosis rate were assessed by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. Briefly, the cells were seeded in six-well plates, treated, and stained with Hoechst 33258 (Beyotime, China, catalog number: C1017) stain, as described in a previous study [ 25 ]. The stained cells were viewed and scored under a fluorescence microscope (IX53, Olympus, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Mitophagy mediated by HIF-1α/FUNDC1 signaling in tubular cells protects against renal ischemia/reperfusion injury

Zhang,

Guo,

et al. 2024

Renal Failure

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Acute kidney injury (AKI) is associated with a high mortality rate. Pathologically, renal ischemia/reperfusion injury (RIRI) is one of the primary causes of AKI, and hypoxia-inducible factor (HIF)-1α may play a defensive role in RIRI. This study assessed the role of hypoxia-inducible factor 1α (HIF-1α)-mediated mitophagy in protection against RIRI in vitro and in vivo . The human tubular cell line HK-2 was used to assess hypoxia/reoxygenation (H/R)-induced mitophagy through different in vitro assays, including western blotting, immunofluorescence staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and reactive oxygen species (ROS) measurement. Additionally, a rat RIRI model was established for evaluation by renal histopathology, renal Doppler ultrasound, and transmission electron microscopy to confirm the in vitro data. The selective HIF-1α inhibitor LW6 reduced H/R-induced mitophagy but increased H/R-induced apoptosis and ROS production. Moreover, H/R treatment enhanced expression of the FUN14 domain-containing 1 (FUNDC1) protein. Additionally, FUNDC1 overexpression reversed the effects of LW6 on the altered expression of light chain 3 (LC3) BII and voltage-dependent anion channels as well as blocked the effects of HIF-1α inhibition in cells. Pretreatment of the rat RIRI model with roxadustat, a novel oral HIF-1α inhibitor, led to decreased renal injury and apoptosis in vivo . In conclusion, the HIF-1α/FUNDC1 signaling pathway mediates H/R-promoted renal tubular cell mitophagy, whereas inhibition of this signaling pathway protects cells from mitophagy, thus aggravating apoptosis, and ROS production. Accordingly, roxadustat may protect against RIRI-related AKI.

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“…The SOD (superoxide dismutase) activity of the P. notoginseng leaves was quantified using a WST-8 assay kit (Suzhou Keming Biotechnology Co., Ltd.) following previous reports ( Cao et al., 2022 ). In brief, 0.1 g of leaves and 1 mL of extraction solution are ground into homogenate on ice, and then centrifuged at 8000 g and 4°C for 10 min, taking the supernatant as the extract.…”

Section: Methodsmentioning

confidence: 99%

Exogenous leucine alleviates heat stress and improves saponin synthesis in Panax notoginseng by improving antioxidant capacity and maintaining metabolic homeostasis

Liu

Yang

et al. 2023

Front. Plant Sci.

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Panax notoginseng saponins (PNSs) are used as industrial raw materials to produce many drugs to treat cardio-cerebrovascular diseases. However, it is a heat-sensitive plant, and its large-scale artificial cultivation is impeded by high temperature stress, leading to decreases in productivity and PNSs yield. Here, we examined exogenous foliar leucine to alleviate heat stress and explored the underlying mechanism using metabolomics. The results indicated that 3 and 5 mM exogenous foliar leucine significantly alleviated heat stress in one-year- and two-year-old P. notoginseng in pots and field trials. Exogenous foliar leucine enhanced the antioxidant capacity by increasing the activities of antioxidant enzymes (POD, SOD) and the contents of antioxidant metabolites (amino acids). Moreover, exogenous foliar leucine enhanced carbohydrate metabolism, including sugars (sucrose, maltose) and TCA cycle metabolites (citric acid, aconitic acid, succinic acid and fumaric acid), in P. notoginseng leaves, stems, and fibrous roots to improve the energy supply of plants and further alleviate heat stress. Field experiments further verified that exogenous foliar leucine increased the productivity and PNSs accumulation in P. notoginseng. These results suggest that leucine application is beneficial for improving the growth and quality of P. notoginseng under heat stress. It is therefore possible to develop plant growth regulators based on leucine to improve the heat resistance of P. notoginseng and other crops.

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Curcumin ameliorates H<sub>2</sub>O<sub>2</sub>-induced injury through SIRT1-PERK-CHOP pathway in pancreatic beta cells

Cited by 9 publications

References 31 publications

Morin-Based Nanoparticles for Regulation of Blood Glucose

Morin-Based Nanoparticles for Regulation of Blood Glucose

Mitophagy mediated by HIF-1α/FUNDC1 signaling in tubular cells protects against renal ischemia/reperfusion injury

Exogenous leucine alleviates heat stress and improves saponin synthesis in Panax notoginseng by improving antioxidant capacity and maintaining metabolic homeostasis

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