2015
DOI: 10.1371/journal.pone.0143223
|View full text |Cite
|
Sign up to set email alerts
|

Cumulus Cells Block Oocyte Meiotic Resumption via Gap Junctions in Cumulus Oocyte Complexes Subjected to DNA Double-Strand Breaks

Abstract: During mammalian oocyte growth, genomic DNA may accumulate DNA double-strand breaks (DSBs) induced by factors such as reactive oxygen species. Recent evidence demonstrated that slight DSBs do not activate DNA damage checkpoint proteins in denuded oocytes. These oocytes, even with DNA DSBs, can resume meiosis and progress to metaphase of meiosis II. Meiotic resumption in oocytes is also controlled by the surrounding cumulus cells; accordingly, we analyzed whether cumulus-cell enclosed oocytes (CEOs) with DNA da… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
20
0
1

Year Published

2016
2016
2024
2024

Publication Types

Select...
8

Relationship

2
6

Authors

Journals

citations
Cited by 29 publications
(23 citation statements)
references
References 21 publications
0
20
0
1
Order By: Relevance
“…An androgen receptor which is responsible for transmitting androgen signals and when knocked out in female mice leads to a lower number of pups per litter26. The candidate genes ADCY1 encoding adenylate cyclase 1 is responsible for cyclizing AMP to form cAMP which takes part in oocyte meiotic arrest and resumption27. DNMT3B a member of the DNA methyltransferases (DNMTs) which appears to play a role in global DNA methylation and in human preimplantation embryo development28.…”
Section: Discussionmentioning
confidence: 99%
“…An androgen receptor which is responsible for transmitting androgen signals and when knocked out in female mice leads to a lower number of pups per litter26. The candidate genes ADCY1 encoding adenylate cyclase 1 is responsible for cyclizing AMP to form cAMP which takes part in oocyte meiotic arrest and resumption27. DNMT3B a member of the DNA methyltransferases (DNMTs) which appears to play a role in global DNA methylation and in human preimplantation embryo development28.…”
Section: Discussionmentioning
confidence: 99%
“…In our previous study, we have demonstrated earlier that the DNA DSBs led to up-regulation of Adcy1 in cumulus cells [17]. To analyze the effect of Adcy1 up-regulation on oocyte nuclear actin filament formation, RNAi-mediated knock-down of Adcy1 expression in cumulus cells was carried out.…”
Section: Resultsmentioning
confidence: 99%
“…However, for denuded oocytes (DO), slight DSBs are unable to activate the DNA damage repair pathway, and the oocytes with partial DSBs could be fertilized when the oocyte developed to the metaphase of the second meiosis [1416]. In our previous study, we found the DNA damage response of oocytes was regulated by their surrounding cumulus cells, however, the mechanism details still haven’t been deep investigated [17]. …”
Section: Introductionmentioning
confidence: 99%
“…There is likely to be a lack of a G2 checkpoint in all mammalian species, not just mice, because porcine oocytes do not appear to initiate a checkpoint either (Wang et al 2015). Recently, it has been suggested that the presence of cumulus cells may allow for oocytes to remain GV arrested when their DNA is damaged (Sun et al 2015).…”
Section: Gv Oocytes Possess a Weak G2/m Checkpointmentioning
confidence: 99%
“…There is likely to be a lack of a G2 checkpoint in all mammalian species, not just mice, because porcine oocytes do not appear to initiate a checkpoint either (Wang et al 2015). Recently, it has been suggested that the presence of cumulus cells may allow for oocytes to remain GV arrested when their DNA is damaged (Sun et al 2015).This could potentially provide some protection against the formation of a fully mature egg with DNA damage in vivo. Nevertheless, the absence of an efficient DNA damage checkpoint in prophase-arrested oocytes is considered to be due to a lack of ATM kinase activation (Marangos & Carroll 2012).…”
mentioning
confidence: 99%