2017
DOI: 10.1371/journal.pone.0170308
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DNA Double-Strand Breaks Induce the Nuclear Actin Filaments Formation in Cumulus-Enclosed Oocytes but Not in Denuded Oocytes

Abstract: As a gamete, oocyte needs to maintain its genomic integrity and passes this haploid genome to the next generation. However, fully-grown mouse oocyte cannot respond to DNA double-strand breaks (DSBs) effectively and it is also unable to repair them before the meiosis resumption. To compensate for this disadvantage and control the DNA repair events, oocyte needs the cooperation with its surrounding cumulus cells. Recently, evidences have shown that nuclear actin filament formation plays roles in cellular DNA DSB… Show more

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Cited by 18 publications
(17 citation statements)
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References 20 publications
(26 reference statements)
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“…Nuclear actin filaments (F-actin) form in response to DSBs in mammalian cells, and have poorly understood functions in repair 1315 . We tested the role of actin polymerization in relocalization of heterochromatic DSBs.…”
Section: Actin Nucleators Mediate Relocalization Of Heterochromatic Dsbsmentioning
confidence: 99%
“…Nuclear actin filaments (F-actin) form in response to DSBs in mammalian cells, and have poorly understood functions in repair 1315 . We tested the role of actin polymerization in relocalization of heterochromatic DSBs.…”
Section: Actin Nucleators Mediate Relocalization Of Heterochromatic Dsbsmentioning
confidence: 99%
“…Interestingly, recent research has revealed that nuclear actin plays an important role in DNA repair processes as well, opening a new domain of research in the nuclear actin field ( Andrin et al, 2012 ; Belin et al, 2015 ; Spichal et al, 2016 ; Caridi et al, 2018 ; Schrank et al, 2018 ). Actin filament formation is induced in the nucleus following DNA double-strand breaks and is thought to have crucial roles in promoting their effective repair ( Andrin et al, 2012 ; Sun et al, 2017 ; Caridi et al, 2018 ; Schrank et al, 2018 ). Moreover, many repair factors such as the nuclease Mre11, recombinase Rad51, and non-homologous end-joining heterodimer Ku70-Ku80 associate with polymerized actin in vitro , and are suggested to help recruit or stabilize these repair factors at the damage site ( Andrin et al, 2012 ).…”
Section: Nuclear Actin In the Dna Damage Responsementioning
confidence: 99%
“…However, DSBs appeared only in oocytes from COCs treated by DMBA, but not in CDOs. This could be explained by the fact that cumulus cells were previously shown to control the DSB response 33 and the DSB-induced formation of nuclear actin filament in oocytes 34 . Thirdly, apoptosis could be induced by DSBs in oocytes 17 , and we found DMBA could induce higher incidence of early apoptosis, but again, only in the COC system.…”
Section: Discussionmentioning
confidence: 99%