Culturing and patch clamping of Jurkat T cells and neurons on Al₂O₃ coated nanowire arrays of altered morphology

Abstract

Report on electrophysiologically intact cell cultures on vertically arranged nanowires as a tool for next generation cell/nanowire stimulation studies.

Toward Brain-on-a-Chip: Human Induced Pluripotent Stem Cell-Derived Guided Neuronal Networks in Tailor-Made 3D Nanoprinted Microscaffolds

“…In detail, we observe a shrinkage between 3% and 16%, which is comparable to the literature for IP-Dip . Since IP-Dip is not explicitly mentioned as biocompatible, we passivated the scaffolds with aluminum oxide to reliably avoid any cytotoxicity. − The complete biocompatibility of our scaffold is reflected in both cell viability and electrophysiology. Despite a small shrinkage of the scaffold, the cavity sizes and the channel diameters reliably served for soma adhesion inside the cavities and neuronal outgrowth inside the channels, as shown via confocal microscopy and scanning electron microscopy.…”

A Temperature-Controlled Patch Clamp Platform Demonstrated on Jurkat T Lymphocytes and Human Induced Pluripotent Stem Cell-Derived Neurons

“…Supplementary Material Figure S1). The gating potential is generally in accordance with the literature [36,38]. The small shift of the threshold from −23.3 to −25.0 mV-an earlier onset-is reasonable, since most proteins have their peak activity at physiological temperatures rather than at RT and the change in channel-conformation that leads to opening of the channels happens faster at 36 • C than at ambient temperatures [39,40].…”

Culturing human iPSC-derived neural progenitor cells on nanowire arrays: mapping the impact of nanowire length and array pitch on proliferation, viability, and membrane deformation

“…Particularly here, unchanged viability was expected since for high NW densities, the cells rest in a fakir-like state on the NWs without any deformation by the NWs. 40,97 In addition, we observed reduced cell numbers on these P1 NW arrays. SEM imaging of these particular samples then showed that the cells form elongations along the array axes.…”

“…[27][28][29][30][31][32][33] Nanowire arrays can affect biological parameters such as cellular growth, viability, morphology, and mechanotransduction machinery, [34][35][36][37][38][39] while electrophysiological parameters are maintained. [40][41][42][43] Furthermore, NW arrays have been used to measure mechanical properties, [44][45][46] to interact with the cell's nucleus, 47 to constrain movement and spreading, [48][49][50][51][52] or to direct cell polarization such as outgrowth of neurites. [53][54][55][56][57] In addition to the aforementioned passive applications, functionalized NW arrays were employed to incorporate an executing role, such as drug delivery, 29,[58][59][60][61][62][63] cell transfection, [64][65][66][67] electrical stimulation/sensing, [68][69][70][71]…”