Culturing human iPSC-derived neural progenitor cells on nanowire arrays: mapping the impact of nanowire length and array pitch on proliferation, viability, and membrane deformation

Harberts, Jann; Bours, Katja; Siegmund, Malte; Hedrich, Carina; Glatza, Michael; Schöler, Hans R.; Haferkamp, Undine; Pleß, Ole; Zierold, Robert; Blick, Robert H.

doi:10.1039/d1nr04352h

Cited by 4 publications

(6 citation statements)

References 112 publications

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“…It can be concluded that each individual BCSC is in close contact with each other on SiNWA due to the limited spreading area compared to the spreading area on the other two substrates. Besides, the mechanical strength and cell deformation are two most critical factors for cell differentiation 29 . Therefore, the cell deformation of BCSCs were stretched by an optical tweezer after 7 days incubation on different substrates.…”

Section: Resultsmentioning

confidence: 99%

“…27 It was shown that geometric parameters and structural optimization of SiNWAs arrays are important signaling modes controlling cell adhesion, morphology, migration, proliferation, and differentiation by transporting exogenous cargos into cells without influencing the cell viability. 28 Harberts, et al 29 showed that SiNW arrays controlled the proliferation and differentiation of human iPSC-derived neural progenitor cells in correlation with the topological difficulty, for example, lengths. Chen, et al 21 reported that SiNW arrays induced the cellular deformations to facilitate gene delivery.…”

Section: Can Inducementioning

confidence: 99%

“…Besides, the mechanical strength and cell deformation are two most critical factors for cell differentiation. 29 Therefore, the cell deformation of BCSCs were stretched by an optical tweezer after 7 days incubation on different substrates. As seen in Figure 5I, the deformation of BCSCs cultured on SiNWA was lower than wafer and culture plate, indicating BCSCs cultured on SiNW arrays were significantly stiffer than the control cells (cultured on culture plate and wafer) under the same stretching force (p < .05).…”

Section: Morphology Of Bcscs On Substratesmentioning

confidence: 99%

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Silicon nanowire array overcomes chemotherapeutic resistance by inducing the differentiation of breast cancer stem cells

Wang

Liu²,

Zhang

et al. 2023

J Biomed Mater Res

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Currently, traditional cancer treatment strategies are greatly challenged by the existence of cancer stem cells (CSCs), which are root cause of chemotherapy resistance.Differentiation therapy presents a novel therapeutic strategy for CSC-targeted therapy. However, there are very few studies on the induction of CSCs differentiation so far. Silicon nanowire array (SiNWA) with many unique properties is considered to be an excellent material for various applications ranging from biotechnology to biomedical applications. In this study, we report the SiNWA differentiates MCF-7-derived breast CSCs (BCSCs) into non-CSCs by modulating the morphology of cells. In vitro, the differentiated BCSCs lose the stemness properties and thus become sensitive to chemotherapeutic drugs, eventually leading to the death of BCSCs. Therefore, this work suggests a potential approach for overcoming chemotherapeutic resistance.

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Section: Resultsmentioning

confidence: 99%

Section: Can Inducementioning

confidence: 99%

Section: Morphology Of Bcscs On Substratesmentioning

confidence: 99%

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Silicon nanowire array overcomes chemotherapeutic resistance by inducing the differentiation of breast cancer stem cells

Wang

Liu²,

Zhang

et al. 2023

J Biomed Mater Res

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“…[ 25 ] In direct comparison to a prior study of us with undifferentiated neural progenitor cells (NPCs)—used to generate the neurons herein—the differentiated neurons were more likely to favor the fakir‐like state at similar specifications of the NW arrays. [ 75 ]…”

Section: Discussionmentioning

confidence: 99%

“…[25] In direct comparison to a prior study of us with undifferentiated neural progenitor cells (NPCs)-used to generate the neurons herein-the differentiated neurons were more likely to favor the fakir-like state at similar specifications of the NW arrays. [75] The neuronal differentiation yields on the NW arrays were analyzed after 14-15 days (8-9 on the NW arrays) of culturing by labeling neuron-specific markers such as MAP2, NeuN, and TH. About 65% of the cells cultivated on the NW arrays were positive for the postmitotic neuronal marker MAP2.…”

Section: Discussionmentioning

confidence: 99%

Generation of Human iPSC‐Derived Neurons on Nanowire Arrays Featuring Varying Lengths, Pitches, and Diameters

Harberts

Siegmund

Hedrich

et al. 2022

Adv Materials Inter

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In this context, vertically aligned high aspect ratio nanostructures-so-called nanowire (NW) arrays-used as cell culture substrates play an increasingly important role in establishing novel tools for interrogating and stimulating cells on molecular and cellular levels. [4][5][6][7] In recent years, studies testing the unique capabilities of NW arrays have been conducted with a variety of cell types, for instance, basic cell lines such as GPE86, HEK293, and HeLa cells, primary rodent neurons, and (mesenchymal) stem cells (MSCs), to name a few. [8,9] The impact of such studies on medical applications such as drug screenings and neurodegenerative disease studies might, however, be significantly improved by employing more sophisticated cells, namely, cells derived from human induced pluripotent stem cells (iPSCs). [10] Human iPSC technologies have changed the way of preclinical research and application by enabling human (patientspecific) in vitro models without restrictions in cell availability. [11] Not only political and ethical controversies raised by using embryonic stem cells (ESCs) are avoided, but also all major cell types including blood-brain barrier models and brain organoids can be generated. [12,13] For studying neurodegenerative diseases such as Alzheimer's or Parkinson's, neurons derived from human iPSCs are of particular interest since adequate models are otherwise scarcely available. [14] Apart from ESCs,

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On-Site Stimulation of Dendritic Cells by Cancer-Derived Extracellular Vesicles on a Core–Shell Nanowire Platform

Zhang,

Ono,

Kawaguchi

et al. 2024

ACS Appl. Mater. Interfaces

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Extracellular vesicles (EVs) contain a subset of proteins, lipids, and nucleic acids that maintain the characteristics of the parent cell. Immunotherapy using EVs has become a focus of research due to their unique features and bioinspired applications in cancer treatment. Unlike conventional immunotherapy using tumor fragments, EVs can be easily obtained from bodily fluids without invasive actions. We previously fabricated nanowire devices that were specialized for EV collection, but they were not suitable for cell culturing. In this study, we fabricated a ZnO/ Al 2 O 3 core−shell nanowire platform that could collect more than 60% of the EVs from the cell supernatant. Additionally, we could continue to culture dendritic cells (DCs) on the platform as an artificial lymph node to investigate cell maturation into antigenpresenting cells. Finally, using this platform, we reproduced a series of on-site immune processes that are among the pivotal immune functions of DCs and include such processes as antigen uptake, antigen presentation, and endocytosis of cancer-derived EVs. This platform provides a new ex vivo tool for EV-DC-mediated immunotherapies.

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Culturing human iPSC-derived neural progenitor cells on nanowire arrays: mapping the impact of nanowire length and array pitch on proliferation, viability, and membrane deformation

Abstract: We tested the growth of human iPSC-derived NPCs on nanowire arrays across a wide range of array characteristics. The proliferation, viability, and interaction with the nanowire arrays were mapped depending on the array pitch and nanowire length.

Cited by 4 publications

References 112 publications

Silicon nanowire array overcomes chemotherapeutic resistance by inducing the differentiation of breast cancer stem cells

Silicon nanowire array overcomes chemotherapeutic resistance by inducing the differentiation of breast cancer stem cells

Generation of Human iPSC‐Derived Neurons on Nanowire Arrays Featuring Varying Lengths, Pitches, and Diameters

On-Site Stimulation of Dendritic Cells by Cancer-Derived Extracellular Vesicles on a Core–Shell Nanowire Platform

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