1972
DOI: 10.1038/bjc.1972.3
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Cultures from Adult Rat Liver Cells. II. Demonstration of Organ-Specific Cell Surface Antigens on Cultured Cells from Normal Liver

Abstract: Summary.-The occurrence of organ-specific antigens on the surface of cells freshly isolated from normal adult rat liver and from liver cell lines cultured as monolayers for up to 6 months is demonstrated. Enzyme treatment used to disaggregate parenchymal cells from liver tissue did not result in appreciable changes in the antigen profile of the cell surface membrane.

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Cited by 28 publications
(9 citation statements)
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“…These cell-lines, which had been in cell culture for 120-150 days were stored frozen before use in these experiments. These control cells have been shown to possess certain antigenic (Iype et al, 1972), morphological Allen et a1., 1978), and biochemical , characteristics of rat liver and did not possess any markers associated with hepatoma cells (Iype and Allen, 1979). The liver cell strain LNRL 1 was isolated from a 13-day-old male Lewis rat by enzymatic digestion using a mixture of Collagenase/Dispase (Boehringer Mannheim Biochemicals, Indianapolis, Indiana).…”
Section: Cell Lines and Culture Methodsmentioning
confidence: 99%
“…These cell-lines, which had been in cell culture for 120-150 days were stored frozen before use in these experiments. These control cells have been shown to possess certain antigenic (Iype et al, 1972), morphological Allen et a1., 1978), and biochemical , characteristics of rat liver and did not possess any markers associated with hepatoma cells (Iype and Allen, 1979). The liver cell strain LNRL 1 was isolated from a 13-day-old male Lewis rat by enzymatic digestion using a mixture of Collagenase/Dispase (Boehringer Mannheim Biochemicals, Indianapolis, Indiana).…”
Section: Cell Lines and Culture Methodsmentioning
confidence: 99%
“…Since that time they have been characterized with respect to karyotype (Chessebeuf et al, 1981;Tsao et al, 1985a), ultrastructure Grisham, 1975;Furukawa et al, 1987), intermediate filaments (Mayer and SchMer, 1982;Marceau et al, 1986;Neupert et al, 1987), membrane antigens (Iype et al, 1972;Marceau et al, 1986), enzyme content Mayer and Schkfer, 1982), amino acid transport (Gebhardt and Williams, 1986) and various cellular and metabolic functions (Tsao et al, 1984;Ismail-Beigi et al, 1986). Despite the considerable variations in phenotypic properties of the different cell lines revealed by these studies, the lines have proved useful for investigating many aspects of liver function, including enzyme regulation (Gerschenson et al, 1974) and metabolism of xenobiotics (Williams, 1981).…”
Section: Introductionmentioning
confidence: 99%
“…Although the epithelial cell lines have maintained some of the functional characteristics of hepatocytes (Borek, 1972;Iype et al, 1972;Weinstein et al, 1975;Odashima et al, 1976;Mayer and Sch~fer, 1982;Herring et al, 1983;Gebhardt and Williams, 1986), there is substantial evidence that the epithelial cells are not derived from hepatocytes (Grisham et al, 1975;Furukawa et al, 1987). However, the exact origin of these cells in the liver is still unresolved.…”
Section: Introductionmentioning
confidence: 99%
“…
Evaluation of hepatic paren'chyma.1 cell metabolic functions in vitro has been attempted utilizing a number of test systems, viz., the perfused liver (1, 2) liver slices (3, 4), andl liver cells isolated by a variety of techniques (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). While studies of the perfused rat liver have been extensive and successful, two1 major disadvantages of this system are (i) it is not exclusively a population of parenchymal cells, and (ii) the number of variables able to be studied simultaneously is limited.
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mentioning
confidence: 99%