1974
DOI: 10.3181/00379727-145-37778
|View full text |Cite
|
Sign up to set email alerts
|

Characterization of Gluconeogenesis in Enzymatically Isolated Parenchymal Cells of Rat Liver

Abstract: Evaluation of hepatic paren'chyma.1 cell metabolic functions in vitro has been attempted utilizing a number of test systems, viz., the perfused liver (1, 2) liver slices (3, 4), andl liver cells isolated by a variety of techniques (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). While studies of the perfused rat liver have been extensive and successful, two1 major disadvantages of this system are (i) it is not exclusively a population of parenchymal cells, and (ii) the number of variables able to be studied simultaneo… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
1
0

Year Published

1977
1977
1981
1981

Publication Types

Select...
4
1

Relationship

0
5

Authors

Journals

citations
Cited by 11 publications
(1 citation statement)
references
References 6 publications
0
1
0
Order By: Relevance
“…Cells were examined prior to the incubation by light microscopy with and without the addition of trypan blue, as described previously (Gumucio et al, 1978b). Gluconeogenesis was assayed according to Cornell and Filkins (1974), using 10 rnM lactate as substrate. Glucose produced during the 2 hr of incubation was measured by the glucose oxydase method (Statzyme glucose, Worthington Biochemical).…”
Section: Experimental Designmentioning
confidence: 99%
“…Cells were examined prior to the incubation by light microscopy with and without the addition of trypan blue, as described previously (Gumucio et al, 1978b). Gluconeogenesis was assayed according to Cornell and Filkins (1974), using 10 rnM lactate as substrate. Glucose produced during the 2 hr of incubation was measured by the glucose oxydase method (Statzyme glucose, Worthington Biochemical).…”
Section: Experimental Designmentioning
confidence: 99%